[field_kinases_species] Recombinant Protein - LRRK2 (1 - 1000) L728D L729D

Name: LRRK2 (1 - 1000) L728D L729D
Price per aliquot (100µg): £110.00
Long Name:
LRRK2 (1 - 1000) L728DL729D
Catalog Number (DU Number):
DU27189
Accession:
NM_198578.3
Expression:
bacteria
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH Agarose
Calculated molecular mass:
Mono-Isotopic Mass: 138, 950.69 daltons
Average Mass: 139, 040.54 daltons
Purity:
>80 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 °C
Theoretical PI:
5.47
Gel Information :
  • Gel Image 1: 
Native Sequence:
Amino acids M1 – L1000 of human LRRK2 (end residue E2527). Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220. The enzyme has a L728D L729D mutation. Residue L728 is equivalent to D959 and L729 is equivalent to D960 of the fusion protein.
Protease Cleavage:
Prescission site (LEVLFQGP) at residues 221 – 228
Cloning Sites:
BamH1 and Not1 site of pGex6P-1

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