Name: LRRK2 (1 - 1000) L728D L729D
Price per aliquot (100µg): £110.00
LRRK2 (1 - 1000) L728DL729D
Catalog Number (DU Number):
Calculated molecular mass:
Mono-Isotopic Mass: 138, 950.69 daltons
Average Mass: 139, 040.54 daltons
50 mM Tris-HCl pH 7.5, 150 mM NaCl, 270 mM sucrose, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Amino acids M1 – L1000 of human LRRK2 (end residue E2527).
Residue M232 of the fusion protein is equivalent to M1 of the native enzyme.
The GST tag is located at residues 1 – 220. The enzyme has a L728D L729D mutation.
Residue L728 is equivalent to D959 and L729 is equivalent to D960 of the fusion protein.
Prescission site (LEVLFQGP) at residues 221 – 228
BamH1 and Not1 site of pGex6P-1