[field_kinases_species] Recombinant Protein - CRHSP 24 (2 - 147)
Name: CRHSP 24 (2 - 147)
Price per aliquot (100µg): £110.00
Catalog Number (DU Number):
Tag and Terminus:
Enzymatic Assay Format:
Substrate for PKB and RSK
Calculated molecular mass:
Mono-Isotopic Mass: 44, 317.37 daltons
Average Mass: 44, 345.96 daltons
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Gel Information :
Gel Image 1:
Amino acids S2 – S147 (end) of human CRHSP 24.
Residue S247 of the fusion protein is equivalent to E2 of the native enzyme. The GST tag is located at residues 1 – 220 and the HA tag is located at residues 238 – 246.
PreScission (LEVLFQGPL) residues 221 - 229
EcoR1 and Sal1site of pGEX 6P-1
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