Polyclonal Antibody - DAZAP

Name: DAZAP
Price per aliquot (100µg): £110.00
Full Name:
DAZAP phospho Thr 315
Long Name Text:
DAZ associated protein
Immuno Sequence:
VPPPPAT*PGAA [residues 309 - 319 of human]
Phosphopeptide:
VPPPPATPGAA [residues 309 - 319 of human]
Sheep Number:
S498B
Monoclonal/Polyclonal:
Polyclonal
Purification Method:
Affinity purified against phospho peptide.
Gel Image:

HEK293 cells were pre-incubated for 1 hour with or without 2 μM PD 184352 or 10 μM U0126, then stimulated with 100 g/ml PMA [panel A] or for 10 mins with 100 ng/ml EGF [panel B] and then lysed. Cell lysates were denatured in SDS, subjected to SDS/PAGE, transferred to nitrocellulose membranes and immunoblotted with an antibody that recognises DAZAP1 phosphorylated at Threonine 315 (anti-DAZAP phospho Thr 315 S498B) [upper panels] and with an antibody that recognises the phosphorylated and unphosphorylated forms of DAZAP1 equally well (anti-DAZAP S470B) [lower panels]. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 10, 000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

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