cDNA Clone CRBN

• Synonyms: MRT2, MRT2A, cereblon, anti-GFP, GFP-binder
• Plasmid: pBABED P FLAG aGFP.16-CRBN
• Parent Plasmid: pBABED P FLAG
• Expressed: FLAG-aGFP.16-5xGLY-CRBN
• Genbank: NP_057386.2
• Tag: FLAG
• Species: Human

Sequence of Insert

aGFP.16
GGATCCATGGCGCAAGTCCAGCTGGTTGAGTCAGGTGGCCGTTTAGTGCAAGCTGGCGATTCCCTGCGCTTGAGCTGTGCAGCGTCAGGACGCACGTTCTCGACTAGCGCAATGGCCTGGTTTCGCCAAGCACCAGGTCGTGAACGCGAGTTTGTAGCCGCGATTACGTGGACCGTTGGGAACACGATTCTGGGCGATTCGGTGAAAGGGCGTTTCACCATCAGTCGCGATCGTGCCAAAAACACCGTCGATCTCCAGATGGACAATCTGGAACCGGAAGATACAGCGGTGTATTACTGCTCGGCTCGTTCTCGGGGTTATGTGCTGTCCGTTTTACGCAGTGTAGACAGCTACGACTATTGGGGACAGGGCACTCAGGTGACCGTTTCTGGCGGTGGAGGCGGAGGTGTCGAC

CRBN
GTCGACATGGCCGGCGAAGGAGATCAGCAGGACGCTGCGCACAACATGGGCAACCACCTGCCGCTCCTGCCTGCAGAGAGTGAGGAAGAAGATGAAATGGAAGTTGAAGACCAGGATAGTAAAGAAGCCAAAAAACCAAACATCATAAATTTTGACACCAGTCTGCCGACATCACATACATACCTAGGTGCTGATATGGAAGAATTTCATGGCAGGACTTTGCACGATGACGACAGCTGTCAGGTGATTCCAGTTCTTCCACAAGTGATGATGATCCTGATTCCCGGACAGACATTACCTCTTCAGCTTTTTCACCCTCAAGAAGTCAGTATGGTGCGGAATTTAATTCAGAAAGATAGAACCTTTGCTGTTCTTGCATACAGCAATGTACAGGAAAGGGAAGCACAGTTTGGAACAACAGCAGAGATATATGCCTATCGAGAAGAACAGGATTTTGGAATTGAGATAGTGAAAGTGAAAGCAATTGGAAGACAAAGGTTCAAAGTCCTTGAGCTAAGAACACAGTCAGATGGAATCCAGCAAGCTAAAGTGCAAATTCTTCCCGAATGTGTGTTGCCTTCAACCATGTCTGCAGTTCAATTAGAATCCCTCAATAAGTGCCAGATATTTCCTTCAAAACCTGTCTCAAGAGAAGACCAATGTTCATATAAATGGTGGCAGAAATACCAGAAGAGAAAGTTTCATTGTGCAAATCTAACTTCATGGCCTCGCTGGCTGTATTCCTTATATGATGCTGAGACCTTAATGGACAGAATCAAGAAACAGCTACGTGAATGGGATGAAAATCTAAAAGATGATTCTCTTCCTTCAAATCCAATAGATTTTTCTTACAGAGTAGCTGCTTGTCTTCCTATTGATGATGTATTGAGAATTCAGCTCCTTAAAATTGGCAGTGCTATCCAGCGACTTCGCTGTGAATTAGACATTATGAATAAATGTACTTCCCTTTGCTGTAAACAATGTCAAGAAACAGAAATAACAACCAAAAATGAAATATTCAGTTTATCCTTATGTGGGCCGATGGCAGCTTATGTGAATCCTCATGGATATGTGCATGAGACACTTACTGTGTATAAGGCTTGCAACTTGAATCTGATAGGCCGGCCTTCTACAGAACACAGCTGGTTTCCTGGGTATGCCTGGACTGTTGCCCAGTGTAAGATCTGTGCAAGCCATATTGGATGGAAGTTTACGGCCACCAAAAAAGACATGTCACCTCAAAAATTTTGGGGCTTAACGCGATCTGCTCTGTTGCCCACGATCCCAGACACTGAAGATGAAATAAGTCCAGACAAAGTAATACTTTGCTTGTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMAQVQLVESGGRLVQAGDSLRLSCAASGRTFSTSAMAWFRQAPGREREFVAAITWTVGNTILGDSVKGRFTISRDRAKNTVDLQMDNLE
PEDTAVYYCSARSRGYVLSVLRSVDSYDYWGQGTQVTVSGGGGGGVDMAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSL
PTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIE
IVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDAETL
MDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPH
GYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU54693
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No