cDNA Clone CRBN

• Synonyms: MRT2, MRT2A, cereblon, anti-GFP, GFP-binder
• Plasmid: pBABED P FLAG CRBN-aGFP16
• Parent Plasmid: pBABED P FLAG
• Expressed: FLAG-CRBN-5xGLY-aGFP.16
• Genbank: NP_057386.2
• Tag: FLAG
• Species: Human

Sequence of Insert

CRBN
GGATCCGCCATGGCCGGCGAAGGAGATCAGCAGGACGCTGCGCACAACATGGGCAACCACCTGCCGCTCCTGCCTGCAGAGAGTGAGGAAGAAGATGAAATGGAAGTTGAAGACCAGGATAGTAAAGAAGCCAAAAAACCAAACATCATAAATTTTGACACCAGTCTGCCGACATCACATACATACCTAGGTGCTGATATGGAAGAATTTCATGGCAGGACTTTGCACGATGACGACAGCTGTCAGGTGATTCCAGTTCTTCCACAAGTGATGATGATCCTGATTCCCGGACAGACATTACCTCTTCAGCTTTTTCACCCTCAAGAAGTCAGTATGGTGCGGAATTTAATTCAGAAAGATAGAACCTTTGCTGTTCTTGCATACAGCAATGTACAGGAAAGGGAAGCACAGTTTGGAACAACAGCAGAGATATATGCCTATCGAGAAGAACAGGATTTTGGAATTGAGATAGTGAAAGTGAAAGCAATTGGAAGACAAAGGTTCAAAGTCCTTGAGCTAAGAACACAGTCAGATGGAATCCAGCAAGCTAAAGTGCAAATTCTTCCCGAATGTGTGTTGCCTTCAACCATGTCTGCAGTTCAATTAGAATCCCTCAATAAGTGCCAGATATTTCCTTCAAAACCTGTCTCAAGAGAAGACCAATGTTCATATAAATGGTGGCAGAAATACCAGAAGAGAAAGTTTCATTGTGCAAATCTAACTTCATGGCCTCGCTGGCTGTATTCCTTATATGATGCTGAGACCTTAATGGACAGAATCAAGAAACAGCTACGTGAATGGGATGAAAATCTAAAAGATGATTCTCTTCCTTCAAATCCAATAGATTTTTCTTACAGAGTAGCTGCTTGTCTTCCTATTGATGATGTATTGAGAATTCAGCTCCTTAAAATTGGCAGTGCTATCCAGCGACTTCGCTGTGAATTAGACATTATGAATAAATGTACTTCCCTTTGCTGTAAACAATGTCAAGAAACAGAAATAACAACCAAAAATGAAATATTCAGTTTATCCTTATGTGGGCCGATGGCAGCTTATGTGAATCCTCATGGATATGTGCATGAGACACTTACTGTGTATAAGGCTTGCAACTTGAATCTGATAGGCCGGCCTTCTACAGAACACAGCTGGTTTCCTGGGTATGCCTGGACTGTTGCCCAGTGTAAGATCTGTGCAAGCCATATTGGATGGAAGTTTACGGCCACCAAAAAAGACATGTCACCTCAAAAATTTTGGGGCTTAACGCGATCTGCTCTGTTGCCCACGATCCCAGACACTGAAGATGAAATAAGTCCAGACAAAGTAATACTTTGCTTGGGTGGCGGAGGCGGAGTCGAC



aGFP.16
GTCGACGCCATGGCGCAAGTCCAGCTGGTTGAGTCAGGTGGCCGTTTAGTGCAAGCTGGCGATTCCCTGCGCTTGAGCTGTGCAGCGTCAGGACGCACGTTCTCGACTAGCGCAATGGCCTGGTTTCGCCAAGCACCAGGTCGTGAACGCGAGTTTGTAGCCGCGATTACGTGGACCGTTGGGAACACGATTCTGGGCGATTCGGTGAAAGGGCGTTTCACCATCAGTCGCGATCGTGCCAAAAACACCGTCGATCTCCAGATGGACAATCTGGAACCGGAAGATACAGCGGTGTATTACTGCTCGGCTCGTTCTCGGGGTTATGTGCTGTCCGTTTTACGCAGTGTAGACAGCTACGACTATTGGGGACAGGGCACTCAGGTGACCGTTTCTGGCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSAMAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVM
MILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPEC
VLPSTMSAVQLESLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACL
PIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTV
AQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCLGGGGGVDAMAQVQLVESGGRLVQAGDSLRLSCAASGRTFSTSAMAW
FRQAPGREREFVAAITWTVGNTILGDSVKGRFTISRDRAKNTVDLQMDNLEPEDTAVYYCSARSRGYVLSVLRSVDSYDYWGQGTQVTVSG*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pBABED P FLAG CRBN-aGFP1681.49 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU54891
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: AdPROM project.; Rockefeller aGFP.16 - DO NOT SEND OUT.
• Low Temp: No