Antibody
PDE3

Full Name
PDE3A phospho Ser 428
Long Name Textual
Phosphodiesterase 3
Immuno Sequence
KRLRRS*LPPGL [residues 423 - 433 of human]
Mono/Poly clonal
Polyclonal
QC Data
Gel Image
PDE3
HeLa cells growing in medium containing serum were serum starved for 12 hours and stimulated with forskolin. Where indicated, cells were incubated with H-7 (10 μM), Ro 318220 (1 μM), PD 184352 (2 μM) or no inhibitor for 1 hour, or BIRB0796 (10 μM) for 2 hours, before stimulation with PMA (400 ng/ml) for a further 15 min. PDE3A was immunoprecipitated (IP) from 3 mg of each cell extract with 20 μg anti-PDE3A (1095 – 1110). The immunoprecipitates were, subjected to SDS-PAGE, transferred to a PVDF membrane and immunoblotted with anti-PDE3A (1095 – 1110) [lower panel] or anti- PDE3A phospho Ser 428 [upper panel]. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti-sheep IgG antibody (1:10.000 dilution, Pierce) followed by enhanced chemiluminescence (ECL, Amersham).

Sheep No
S446B
Aliquot
0.1mg
Price
£125.00