Full Name
IRAK2
Long Name Textual
interleukin-1 receptor-associated kinase 2
Synonyms
IRAK2
Immuno Sequence
GST-IRAK2 [DU 15028]
Mono/Poly clonal
Polyclonal
Gel Image
Raw (264.7) cell extracts (0.5 mg protein) were immunoprecipitated with increasing amounts of sheep anti-IRAK2 antibody (lanes 1 - 5) or pre-immune serum (lane 6) overnight, followed by incubation for 1 hour with 15 μl of protein G-Sepharose. Immunoprecipitated proteins were denatured in SDS and subjected to SDS-PAGE on 8% gels. Lanes 1 - 6 show the amount of mouse IRAK2 that has been immunoprecipitated with the respective amount of antibody, whereas the lanes 7-12 indicates the amount of mouse IRAK2 protein remaining in the supernatant after the immunoprecipitation. After SDS-PAGE, the proteins were transferred to PVDF membrane and immunoblotting was carried out using the rabbit polyclonal antibody against IRAK2 (Abcam, ab62419) overnight at an antibody concentration of 1:1000. The result shows that nearly all the IRAK2 can be depleted from the extract using this antibody.
Sheep No
S479C
Unit Source
Aliquot
0.1mg
Price
£125.00