Full Name
KEAP1 (GST cleaved)
Long Name Textual
kelch-like ECH-associated protein 1
Synonyms
KEAP1
Immuno Sequence
KEAP1 (GST cleaved) [DU 23204]
Mono/Poly clonal
Polyclonal
Gel Image
Keap1 antibody bleed 2, and KEAP1 siRNA Dharmacon (LU-012453-00-0002 ON-TARGET plus) U20S cell extracts (40 μg of protein) were subject to SDS-PAGE and transferred to nitrocellulose membrane, and blotted with anti-Keap1 at 1 μg/mL overnight at 4°C, anti-sheep secondary at 1:10 000
Keap1 antibodies were covalently coupled to protein G Sepharose in a ratio of 1 mg of antibody to 1 mL of resin using a DMP cross-linking procedure. Following cross-linking, cellular extract (1 mg protein) was incubated with 20 μL coupled antibody resin at 4°C for 2 h. The resin was then washed with Cell lysis buffer twice, and 50 mM Tris 150 mM NaCl twice. The resin was resuspended in Laemmli buffer and subject to SDS PAGE followed by transfer to nitrocellulose and blotting with anti-Keap1 (bleed1).
Sheep No
S463D
Unit Source
Aliquot
0.1mg
Price
£125.00