Full Name
SV2A phospho Ser 42 + Ser 45 + Ser 47
Long Name Textual
synaptic vesicle glycoprotein 2A
Synonyms
SV2A
Immuno Sequence
CKKRVQDEYS*RRS*YS*RFEEEDDKK [residues 36 - 54 of human]
Mono/Poly clonal
Polyclonal
Gel Image
![SV2A](/sites/default/files/styles/gel_image/public/gel_images/SV2A_Ser42-Ser45-Ser47_S287D_IB_1.jpg?itok=e_tBf4on)
![Purified GST-SV2A (1-160) protein was phosphorylated in vitro by purified GST- TTBK2 (1 - 316) WT and GST-TTBK2 (1 - 316) KD. Increasing amounts of phosphorylated GST-SV2A (1 - 160) (0 ng, 10 ng, 50 ng, 100 ng) were subjected to SDS-PAGE and each phosphospecific antibody was tested by their ability to detect those phosphosites. Faint non-specific detection of phosphosites in kinase-inactive reaction sample. Potentially useful antibody. Therefore, further testing carried out to find the best bleed(s). HEK-293 cells stably expressing GFP-tagged full-length TTBK2 wild-type and kinase dead were transfected with GST-SV2A(1-160) and 20 μg of each lysate was immunoblotted with each antibody to be tested.](/sites/default/files/styles/gel_image/public/gel_images/SV2A_Ser42-ser45-Ser47_S287D_IB_2.jpg?itok=Nn1cV9m7)
Purified GST-SV2A (1-160) protein was phosphorylated in vitro by purified GST- TTBK2 (1 - 316) WT and GST-TTBK2 (1 - 316) KD. Increasing amounts of phosphorylated GST-SV2A (1 - 160) (0 ng, 10 ng, 50 ng, 100 ng) were subjected to SDS-PAGE and each phosphospecific antibody was tested by their ability to detect those phosphosites. Faint non-specific detection of phosphosites in kinase-inactive reaction sample. Potentially useful antibody. Therefore, further testing carried out to find the best bleed(s). HEK-293 cells stably expressing GFP-tagged full-length TTBK2 wild-type and kinase dead were transfected with GST-SV2A(1-160) and 20 μg of each lysate was immunoblotted with each antibody to be tested.
Sheep No
S287D
Unit Source
Aliquot
0.1mg
Price
£125.00