cDNA Clone FAF1

• Synonyms: hFAF1, CGI-03, HFAF1s, UBXD12, UBXN3A, FLJ37524
• Plasmid: pCMV-FLAG FAF1 P620G
• Parent Plasmid: pCMV5 FLAG3
• Expressed: FLAG-FAF1 P620G
• Genbank: NM_007051.2*
• Tag: FLAG
• Species: Human

Sequence of Insert

GTCGACGCGTCCAACATGGACCGGGAGATGATCCTGGCGGATTTTCAGGCATGTACTGGCATTGAAAACATTGACGAAGCTATTACATTGCTTGAACAAAATAATTGGGACTTAGTGGCAGCTATCAATGGTGTAATACCACAGGAAAATGGCATTCTACAAAGTGAATATGGAGGTGAGACCATACCAGGACCTGCATTTAATCCAGCAAGTCATCCAGCTTCAGCTCCTACTTCCTCTTCTTCTTCAGCGTTTCGACCTGTAATGCCATCCAGGCAGATTGTAGAAAGGCAACCTCGGATG
CTGGACTTCAGGGTTGAATACAGAGACAGAAATGTTGATGTGGTACTTGA
AGACACCTGTACTGTTGGAGAGATTAAACAGATTCTAGAAAATGAACTTC
AGATACCTGTGTCCAAAATGCTGTTAAAAGGCTGGAAGACGGGAGATGTG
GAAGACAGTACGGTCCTAAAATCTCTACACTTGCCAAAAAACAACAGTCT
TTATGTCCTTACACCAGATTTGCCACCACCTTCATCATCTAGTCATGCTG
GTGCCCTGCAGGAGTCATTAAATCAAAACTTCATGCTGATCATCACCCAC
CGAGAAGTCCAGCGGGAGTACAACCTGAACTTCTCAGGAAGCAGTACTAT
TCAAGAGGTAAAGAGAAATGTGTATGACCTTACAAGTATCCCCGTTCGCC
ACCAATTATGGGAGGGCTGGCCAACTTCTGCTACAGACGACTCAATGTGT
CTTGCTGAATCAGGGCTCTCTTATCCCTGCCATCGACTTACAGTGGGAAG
AAGATCTTCACCTGCACAGACCCGGGAACAGTCGGAAGAACAAATCACCG
ATGTTCATATGGTTAGTGATAGCGATGGAGATGACTTTGAAGATGCTACA
GAATTTGGGGTGGATGATGGAGAAGTATTTGGCATGGCGTCATCTGCCTT
GAGAAAATCTCCAATGATGCCAGAAAACGCAGAAAATGAAGGAGATGCCT
TATTACAATTTACAGCAGAGTTTTCTTCAAGATATGGTGATTGCCATCCT
GTATTTTTTATTGGCTCATTAGAAGCTGCTTTTCAAGAGGCCTTCTATGT
GAAAGCCCGAGATAGAAAGCTTCTTGCTATCTACCTCCACCATGATGAAA
GTGTGTTAACCAACGTGTTCTGCTCACAAATGCTTTGTGCTGAATCCATT
GTTTCTTATCTGAGTCAAAATTTTATAACCTGGGCTTGGGATCTGACAAA
GGACTCCAACAGAGCAAGATTTCTCACTATGTGCAATAGACACTTTGGCA
GTGTTGTGGCACAAACCATTCGGACTCAAAAAACGGATCAGTTTCCGCTT
TTCCTGATTATTATGGGAAAGCGATCATCTAATGAAGTGTTGAATGTGAT
ACAAGGGAACACAACAGTAGATGAGTTAATGATGAGACTCATGGCTGCAA
TGGAGATCTTCACAGCCCAACAACAGGAAGATATAAAGGACGAGGATGAA
CGTGAAGCCAGAGAAAATGTGAAGAGAGAGCAAGATGAGGCCTATCGCCT
TTCACTTGAGGCTGACAGAGCAAAGAGGGAAGCTCACGAGAGAGAGATGG
CAGAACAGTTTCGTTTGGAGCAGATTCGCAAAGAACAAGAAGAGGAACGT
GAGGCCATCCGGCTGTCCTTAGAGCAAGCCCTGCCTCCTGAGCCAAAGGA
AGAAAATGCTGAGCCTGTGAGCAAACTGCGGATCCGGACCCCCAGTGGCG
AGTTCTTGGAGCGGCGTTTCCTGGCCAGCAACAAGCTCCAGATTGTCTTT
GATTTTGTAGCTTCCAAAGGATTTCCATGGGATGAGTACAAGTTACTGAG
CACCTTTGGTAGGAGAGACGTAACTCAACTGGACCCAAATAAATCATTAT
TGGAGGTAAAGTTGTTCCCTCAAGAAACCCTTTTCCTTGAAGCAAAAGAG
TAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGDPRNSRVDASNMDREMILADFQACTGIENIDEAITLLEQN
NWDLVAAINGVIPQENGILQSEYGGETIPGPAFNPASHPASAPTSSSSSA
FRPVMPSRQIVERQPRMLDFRVEYRDRNVDVVLEDTCTVGEIKQILENEL
QIPVSKMLLKGWKTGDVEDSTVLKSLHLPKNNSLYVLTPDLPPPSSSSHA
GALQESLNQNFMLIITHREVQREYNLNFSGSSTIQEVKRNVYDLTSIPVR
HQLWEGWPTSATDDSMCLAESGLSYPCHRLTVGRRSSPAQTREQSEEQIT
DVHMVSDSDGDDFEDATEFGVDDGEVFGMASSALRKSPMMPENAENEGDA
LLQFTAEFSSRYGDCHPVFFIGSLEAAFQEAFYVKARDRKLLAIYLHHDE
SVLTNVFCSQMLCAESIVSYLSQNFITWAWDLTKDSNRARFLTMCNRHFG
SVVAQTIRTQKTDQFPLFLIIMGKRSSNEVLNVIQGNTTVDELMMRLMAA
MEIFTAQQQEDIKDEDEREARENVKREQDEAYRLSLEADRAKREAHEREM
AEQFRLEQIRKEQEEEREAIRLSLEQALPPEPKEENAEPVSKLRIRTPSG
EFLERRFLASNKLQIVFDFVASKGFPWDEYKLLSTFGRRDVTQLDPNKSL
LEVKLFPQETLFLEAKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20795
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *No native initiating methionine; Mammalian expression vector for FLAG-FAF1 P620G mutant