cDNA Clone Cullin4b

• Synonyms: CUL4B
• Plasmid: pSC-B-Cullin4b
• Parent Plasmid: pSC-B
• Genbank: NM_003588
• Species: Human

Sequence of Insert

GGATCCATGATGTCACAGTCATCTGGATCAGGAGATGGGAATGATGATGAGGCTACTACCTCTAAAGACGGTGGTTTTTCTTCCCCCAGTCCCTCAGCTGCTGCTGCTGCTCAGGAGGTCAGATCTGCCACTGATGGTAATACCAGCACCACTCCGCCCACCTCTGCCAAGAAGAGAAAGTTAAACAGCAGCAGCAGTAGCAGCAGTAACAGTAGTAACGAGAGAGAAGACTTTGATTCCACCTCTTCCTCCTCTTCCACTCCTCCTTTACAACCCAGGGATTCGGCATCCCCTTCAACCTCGTCC
TTCTGCCTGGGGGTTTCAGTGGCTGCTTCCAGCCACGTACCGATACAGAA
GAAGCTGCGTTTTGAAGACACCCTGGAGTTTGTAGGGTTTGATGCGAAGA
TGGCTGAGGAATCCTCCTCCTCCTCCTCCTCATCTTCACCAACTGCTGCA
ACATCTCAGCAGCAGCAACTTAAAAATAAGAGTATATTAATCTCTTCTGT
GGCTTCGGTGCATCATGCAAACGGCCTAGCCAAATCTTCTACCACCGTCT
CTAGCTTTGCTAACAGCAAACCTGGCTCTGCTAAGAAGTTAGTGATCAAG
AACTTTAAAGATAAGCCTAAATTACCAGAAAACTACACAGATGAAACCTG
GCAAAAACTGAAAGAAGCAGTGGAAGCTATTCAGAATAGTACTTCAATTA
AGTACAATTTAGAAGAACTCTACCAGGCTGTAGAAAATCTCTGTTCTTAC
AAGATTTCTGCAAACTTGTACAAACAGCTGAGACAGATCTGCGAAGATCA
CATCAAAGCACAGATTCATCAATTCAGAGAGGATTCATTGGATAGCGTTC
TTTTTTTAAAGAAGATTGATAGATGCTGGCAAAACCATTGCAGACAAATG
ATCATGATCAGGAGCATTTTTTTGTTTCTGGATAGAACTTACGTTCTTCA
GAATTCAATGCTACCCTCCATTTGGGACATGGGACTGGAGTTATTTAGGG
CTCATATTATAAGTGATCAGAAAGTGCAGAATAAGACAATTGATGGCATT
CTTCTCTTGATTGAGAGGGAAAGGAATGGTGAAGCAATTGATAGAAGTTT
ACTTCGAAGCCTTTTAAGCATGCTGTCTGATTTGCAAATTTATCAAGATT
CTTTTGAACAACGATTTTTGGAAGAAACTAACCGGCTCTATGCAGCTGAA
GGCCAAAAATTAATGCAAGAAAGAGAGGTTCCTGAATATCTACATCATGT
TAACAAACGTCTAGAAGAAGAAGCAGACAGACTTATTACTTACTTAGATC
AGACCACCCAGAAGTCATTAATTGCTACTGTAGAAAAACAACTTCTAGGT
GAACACTTAACAGCAATTCTTCAGAAAGGTTTAAATAACCTCCTTGATGA
AAACCGAATTCAAGATTTGTCTCTTCTGTATCAGCTCTTCAGTAGAGTTC
GAGGTGGAGTTCAGGTTCTTTTGCAGCAGTGGATCGAATATATCAAGGCA
TTTGGCAGCACTATTGTAATTAATCCTGAAAAAGATAAAACCATGGTTCA
AGAATTGCTGGATTTTAAAGATAAGGTTGACCATATAATTGATATCTGCT
TTCTGAAGAATGAGAAATTTATCAATGCCATGAAAGAAGCATTTGAAACG
TTCATTAACAAAAGACCAAATAAACCAGCTGAACTTATAGCTAAGTATGT
AGATTCAAAACTTCGTGCAGGCAACAAAGAAGCTACAGATGAAGAACTTG
AGAAAATGTTGGATAAAATTATGATCATATTTAGATTTATCTATGGCAAG
GATGTTTTTGAGGCCTTCTATAAGAAAGATTTAGCCAAGCGCCTGTTAGT
CGGAAAGAGTGCATCTGTAGATGCTGAAAAATCAATGCTGTCCAAACTTA
AACATGAATGCGGAGCTGCTTTCACCAGCAAACTTGAAGGAATGTTTAAA
GACATGGAACTTTCTAAAGACATCATGATTCAGTTCAAACAGTATATGCA
GAATCAGAATGTTCCGGGAAATATTGAGTTAACTGTGAATATCCTGACAA
TGGGCTATTGGCCGACATATGTGCCTATGGAAGTTCATTTACCACCAGAG
ATGGTAAAACTTCAGGAGATTTTCAAGACATTTTACCTAGGCAAACATAG
TGGCAGGAAACTTCAGTGGCAGTCAACCCTAGGACACTGTGTGTTAAAAG
CAGAATTTAAAGAGGGTAAAAAGGAACTCCAGGTCTCTCTTTTTCAAACA
CTGGTGCTGCTAATGTTTAATGAGGGAGAGGAGTTCAGTTTAGAAGAGAT
CAAGCAGGCAACTGGAATAGAGGATGGAGAGTTAAGGAGAACACTGCAGT
CATTAGCCTGTGGCAAAGCTAGAGTTCTGGCGAAAAATCCAAAGGGCAAA
GACATTGAAGATGGTGACAAGTTCATTTGTAATGATGATTTCAAACATAA
ACTTTTCAGGATAAAGATCAATCAAATCCAGATGAAAGAAACGGTTGAAG
AACAAGCAAGCACTACAGAAAGAGTATTTCAAGACAGACAGTATCAAATT
GATGCTGCAATTGTTCGAATTATGAAGATGAGAAAGACACTTAGCCACAA
TCTCCTTGTTTCAGAAGTGTACAACCAGTTGAAATTTCCAGTAAAGCCTG
CTGATCTTAAGAAGAGAATAGAATCTTTAATTGACCGGGACTACATGGAA
AGAGATAAAGAAAATCCAAACCAGTACAACTATATTGCATAGGCGGCCGC

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20034
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Source plasmid for Cullin 4b.; IMAGE clone source carries a c2179a mutation conferring Leu727Ile. This was corrected in this clone using mutagenic oligos 55184/55185.