cDNA Clone N4BP1

• Plasmid: pGEX6P-1-HALO_TEV-N4BP1
• Parent Plasmid: pGEX6P1 Halo TEV
• Expressed: HALO_TEV-N4BP1
• Genbank: Gene ID 9683 NM_153029
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCatggcggcccgggcggtgctggacgagttcactgcgccagctga
gaaggcggagctgctggagcagagccgcggccgtatcgagggcctgtttg
gcgtgagcctagccgtgctcggcgcgctaggggctgaggagccgctgccc
gcgcgcatctggctgcagctctgcggggcgcaggaggcggtgcacagcgc
caaggaatatattaaaggaatctgtgaacctgaactagaagaaagagaat
gttaccccaaggacatgcactgcatttttgttggggcagagagcctgttt
ctgaaaagcttgattcaggatacttgtgctgacctctgcattctggacat
tggccttcttggcatcagaggaagtgctgaggctgtggtcatggctagga
gtcacattcaacaatttgtaaagctctttgaaaataaagagaacctaccc
agtagtcagaaagaatcagaggtgaaaagggaattcaaacaatttgttga
agcccatgcagacaattacacaatggatttgttgattttgcccacttcct
tgaaaaaagaacttttgacactcacacaaggtgaggagaatctctttgaa
acaggagatgatgaggttattgaaatgagagattctcaacaaacagagtt
tacacagaatgctgccacagggctgaatatttctagagatgaaactgttt
tgcaggaagaggcaagaaataaagctgggactcctgtttctgagcttaca
aaacaaatggacacagtcctttctagctcaccagatgtgctttttgatcc
aataaatggtctaaccccagatgaagaggcactttccaatgagagaattt
gtcagaaaaggagattttctgattctgaagaaaggcatacgaagaagcag
ttttctttggaaaatgttcaggagggggagattttacacgatgctaagac
attggctggaaatgtaatagctgacctatctgattcttctgctgattctg
aaaatttaagtccagatataaaagaaactactgaggaaatggaatacaac
atcctcgtaaacttttttaaaaccatgggctactcccaagaaattgttga
aaaggtcattaaggtgtatggaccatctactgaaccattattgctcttag
aggaaattgaaaaagaaaataaaagattccaagaagacagagaattttca
gctggtacagtgtatccagagaccaacaaaaccaaaaataaaggtgttta
tagcagcacaaatgagcttacaactgattccactccaaagaaaacacagg
ctcacacacagcaaaatatggtagaaaaattttctcagttaccattcaaa
gtggaagctaaaccatgtacctcaaattgtagaattaatactttcagaac
agtgccaatagaacagaaacatgaagtctggggttcaaaccagaactaca
tttgtaacacagaccctgaaactgatggcctttcaccttctgttgcctct
ccaagtcccaaagaagtcaattttgtttcaaggggagcttcaagtcacca
gcccagagttccactttttcctgaaaatggtttacaccagcagccagaac
ccttgcttccaaataatatgaaatctgcctgtgaaaaacgtttaggatgt
tgtagttctcctcattctaagccaaattgctcaaccctttctccaccaat
gccactgccccagctgttaccttcggttactgatgcaaggtcggcaggac
cttctgatcatattgattcctcagttactggggttcaaaggtttcgagat
actctaaaaataccctacaagctggaattaaaaaatgaaccagggagaac
ggatttgaaacacattgttatagatgggagcaatgttgcaattacccatg
gtctgaaaaagttcttttcttgtcgtggaattgcaattgcagttgaatat
ttttggaagcttggcaacagaaacatcactgtatttgtccctcagtggag
aacaaggcgtgatcctaatgtcacagaacagcacttcttaacccagctcc
aggagctcggaatattatctttaactcctgcccggatggtctttggagaa
agaattgcttctcatgatgacaggtttctactacacttagcggacaaaac
tggtggcataattgtgacaaatgataatttcagagaatttgtgaatgagt
cagtctcttggagagaaattattacaaaaaggctgctgcagtacacgttc
gtgggggacatatttatggttcccgatgatcctctgggaagaagtggacc
tcgattagaggagtttcttcagaaggaagtctgtcttagagatatgcagc
ccctactcagtgccctgccaaatgtgggcatgtttgaccccagcttcaga
gtccctggcacccaggcagccagcaccagccaccagcctccgacccggat
tcagggagccccttcaagccactggctccctcagcagccccacttcccac
tgctgccagccctccccagtctccagcagaacctgcccatgccagctcag
agatcttctgcagaaaccaacgagctgagggaagcccttctgaagatctt
ccctgactcagagcaaagactgaaaatagaccagatcttggtggcccacc
catacatgaaagatctaaatgcgctttctgccatggtgttggattgaGCG
GCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGENLYFQGGSMAARAVLDEFTAP
AEKAELLEQSRGRIEGLFGVSLAVLGALGAEEPLPARIWLQLCGAQEAVH
SAKEYIKGICEPELEERECYPKDMHCIFVGAESLFLKSLIQDTCADLCIL
DIGLLGIRGSAEAVVMARSHIQQFVKLFENKENLPSSQKESEVKREFKQF
VEAHADNYTMDLLILPTSLKKELLTLTQGEENLFETGDDEVIEMRDSQQT
EFTQNAATGLNISRDETVLQEEARNKAGTPVSELTKQMDTVLSSSPDVLF
DPINGLTPDEEALSNERICQKRRFSDSEERHTKKQFSLENVQEGEILHDA
KTLAGNVIADLSDSSADSENLSPDIKETTEEMEYNILVNFFKTMGYSQEI
VEKVIKVYGPSTEPLLLLEEIEKENKRFQEDREFSAGTVYPETNKTKNKG
VYSSTNELTTDSTPKKTQAHTQQNMVEKFSQLPFKVEAKPCTSNCRINTF
RTVPIEQKHEVWGSNQNYICNTDPETDGLSPSVASPSPKEVNFVSRGASS
HQPRVPLFPENGLHQQPEPLLPNNMKSACEKRLGCCSSPHSKPNCSTLSP
PMPLPQLLPSVTDARSAGPSDHIDSSVTGVQRFRDTLKIPYKLELKNEPG
RTDLKHIVIDGSNVAITHGLKKFFSCRGIAIAVEYFWKLGNRNITVFVPQ
WRTRRDPNVTEQHFLTQLQELGILSLTPARMVFGERIASHDDRFLLHLAD
KTGGIIVTNDNFREFVNESVSWREIITKRLLQYTFVGDIFMVPDDPLGRS
GPRLEEFLQKEVCLRDMQPLLSALPNVGMFDPSFRVPGTQAASTSHQPPT
RIQGAPSSHWLPQQPHFPLLPALPSLQQNLPMPAQRSSAETNELREALLK
IFPDSEQRLKIDQILVAHPYMKDLNALSAMVLD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24030
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental: 24005