cDNA Clone RIOK3

• Plasmid: pGEX-C3-HALO_Thr-RIOK3
• Parent Plasmid: pGEX6P Halo Thr
• Expressed: GST-HALO-RIOK3
• Genbank: AAH39729.1
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCatggatctggtaggagtggcatcgcctgagcccgggacggcagc
ggcctggggacccagcaagtgtccatgggctattcctcaaaatacaatat
cttgttctttggctgatgtaatgagtgaacagctggccaaagaattgcag
ttagaagaagaagctgccgtttttcctgaagttgctgttgctgaaggacc
atttattactggagaaaacattgatacttccagtgaccttatgctggctc
agatgctacagatggaatatgacagagaatatgatgcacagcttaggcgt
gaagaaaaaaaattcaatggagatagcaaagtttccatttcctttgaaaa
ttatcgaaaagtgcatccttatgaagacagcgatagctctgaagatgagg
ttgactggcaggatactcgtgatgatccctacagaccagcaaaaccggtt
cccactcctaaaaagggctttattggaaaaggaaaagatatcaccaccaa
acatgatgaagtagtatgtgggagaaagaacacagcaagaatggaaaatt
ttgcacctgagtttcaggtaggagatggaattggaatggatttaaaacta
tcaaaccatgttttcaatgctttaaaacaacatgcctactcagaagaacg
tcgaagtgcccgcctacatgagaaaaaggagcattctacagcagaaaaag
cagttgatcctaagacacgtttacttatgtataaaatggtcaactctgga
atgttggagacaatcactggctgtattagtacaggaaaggagtctgttgt
ctttcatgcatatggagggagcatggaggatgaaaaggaagatagtaaag
ttatacctacagaatgtgccatcaaggtatttaaaacaacccttaatgaa
tttaagaatcgtgacaaatatattaaagatgatttcaggtttaaagatcg
cttcagtaaactaaatccacgtaagatcatccgcatgtgggcagaaaaag
aaatgcacaatctcgcaagaatgcagagagctggaattccttgtccaaca
gttgtactactgaagaaacacattttagttatgtcttttattggccatga
tcaagttccagcccctaaattaaaagaagtaaagctcaatagtgaagaaa
tgaaagaagcctactatcaaactcttcatttgatgcggcagttatatcat
gaatgtacgcttgtccatgctgacctcagtgagtataacatgctgtggca
tgctggaaaggtctggttgatcgatgtcagtcagtcagtagaacctaccc
accctcacggcctggagttcttgttccgggactgcaggaatgtctcgcag
aaaggaggagtcaaggaagcccttagtgaacgagaactcttcaatgctgt
ttcaggcttaaacatcacagcagataatgaagctgattttttagctgaga
tagaagctttggagaaaatgaatgaagatcacgttcagaagaatggaagg
aaagctgcttcatttttgaaagatgatggagacccaccactactatatga
tgaatagGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMDLVGVASPEPGTA
AAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEG
PFITGENIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFE
NYRKVHPYEDSDSSEDEVDWQDTRDDPYRPAKPVPTPKKGFIGKGKDITT
KHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHAYSEE
RRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESV
VFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKD
RFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGH
DQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLW
HAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNA
VSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLY
DE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24211
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental: 24192