cDNA Clone N4BP1 aa209-896

• Synonyms: NEDD4 binding protein 1
• Plasmid: pGEX HALO-Thr N4BP1 aa209-896
• Parent Plasmid: pGEX6P Halo Thr
• Expressed: GST-HALO-N4BP1 aa209-896
• Genbank: NM_153029.3
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCGATTCTCAACAAACAGAGTTTACACAGAATGCTGCCACAGGGCTGAATATTTCTAGAGATGAAACTGTTTTGCAGGAAGAGGCAAGAAATAAAGCTGGGACTCCTGTTTCTGAGCTTACAAAACAAATGGACACAGTCCTTTCTAGCTCACCAGATGTGCTTTTTGATCCAATAAATGGTCTAACCCCAGATGAAGAGGCACTTTCCAATGAGAGAATTTGTCAGAAAAGGAGATTTTCTGATTCTGAAGAAAGGCATACGAAGAAGCAGTTTTCTTTGGAAAATGTTCAGGAGGGGGAGATTTTACACGATGCTAAGACATTGGCTGGAAATGTAATAGCTGACCTATCTGATTCTTCTGCTGATTCTGAAAATTTAAGTCCAGATATAAAAGAAACTACTGAGGAAATGGAATACAACATCCTCGTAAACTTTTTTAAAACCATGGGCTACTCCCAAGAAATTGTTGAAAAGGTCATTAAGGTGTATGGACCATCTACTGAACCATTATTGCTCTTAGAGGAAATTGAAAAAGAAAATAAAAGATTCCAAGAAGACAGAGAATTTTCAGCTGGTACAGTGTATCCAGAGACCAACAAAACCAAAAATAAAGGTGTTTATAGCAGCACAAATGAGCTTACAACTGATTCCACTCCAAAGAAAACACAGGCTCACACACAGCAAAATATGGTAGAAAAATTTTCTCAGTTACCATTCAAAGTGGAAGCTAAACCATGTACCTCAAATTGTAGAATTAATACTTTCAGAACAGTGCCAATAGAACAGAAACATGAAGTCTGGGGTTCAAACCAGAACTACATTTGTAACACAGACCCTGAAACTGATGGCCTTTCACCTTCTGTTGCCTCTCCAAGTCCCAAAGAAGTCAATTTTGTTTCAAGGGGAGCTTCAAGTCACCAGCCCAGAGTTCCACTTTTTCCTGAAAATGGTTTACACCAGCAGCCAGAACCCTTGCTTCCAAATAATATGAAATCTGCCTGTGAAAAACGTTTAGGATGTTGTAGTTCTCCTCATTCTAAGCCAAATTGCTCAACCCTTTCTCCACCAATGCCACTGCCCCAGCTGTTACCTTCGGTTACTGATGCAAGGTCGGCAGGACCTTCTGATCATATTGATTCCTCAGTTACTGGGGTTCAAAGGTTTCGAGATACTCTAAAAATACCCTACAAGCTGGAATTAAAAAATGAACCAGGGAGAACGGATTTGAAACACATTGTTATAGATGGGAGCAATGTTGCAATTACCCATGGTCTGAAAAAGTTCTTTTCTTGTCGTGGAATTGCAATTGCAGTTGAATATTTTTGGAAGCTTGGCAACAGAAACATCACTGTATTTGTCCCTCAGTGGAGAACAAGGCGTGATCCTAATGTCACAGAACAGCACTTCTTAACCCAGCTCCAGGAGCTCGGAATATTATCTTTAACTCCTGCCCGGATGGTCTTTGGAGAAAGAATTGCTTCTCATGATGACAGGTTTCTACTACACTTAGCGGACAAAACTGGTGGCATAATTGTGACAAATGATAATTTCAGAGAATTTGTGAATGAGTCAGTCTCTTGGAGAGAAATTATTACAAAAAGGCTGCTGCAGTACACGTTCGTGGGGGACATATTTATGGTTCCCGATGATCCTCTGGGAAGAAGTGGACCTCGATTAGAGGAGTTTCTTCAGAAGGAAGTCTGTCTTAGAGATATGCAGCCCCTACTCAGTGCCCTGCCAAATGTGGGCATGTTTGACCCCAGCTTCAGAGTCCCTGGCACCCAGGCAGCCAGCACCAGCCACCAGCCTCCGACCCGGATTCAGGGAGCCCCTTCAAGCCACTGGCTCCCTCAGCAGCCCCACTTCCCACTGCTGCCAGCCCTCCCCAGTCTCCAGCAGAACCTGCCCATGCCAGCTCAGAGATCTTCTGCAGAAACCAACGAGCTGAGGGAAGCCCTTCTGAAGATCTTCCCTGACTCAGAGCAAAGACTGAAAATAGACCAGATCTTGGTGGCCCACCCATACATGAAAGATCTAAATGCGCTTTCTGCCATGGTGTTGGATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSDSQQTEFTQNAATG
LNISRDETVLQEEARNKAGTPVSELTKQMDTVLSSSPDVLFDPINGLTPD
EEALSNERICQKRRFSDSEERHTKKQFSLENVQEGEILHDAKTLAGNVIA
DLSDSSADSENLSPDIKETTEEMEYNILVNFFKTMGYSQEIVEKVIKVYG
PSTEPLLLLEEIEKENKRFQEDREFSAGTVYPETNKTKNKGVYSSTNELT
TDSTPKKTQAHTQQNMVEKFSQLPFKVEAKPCTSNCRINTFRTVPIEQKH
EVWGSNQNYICNTDPETDGLSPSVASPSPKEVNFVSRGASSHQPRVPLFP
ENGLHQQPEPLLPNNMKSACEKRLGCCSSPHSKPNCSTLSPPMPLPQLLP
SVTDARSAGPSDHIDSSVTGVQRFRDTLKIPYKLELKNEPGRTDLKHIVI
DGSNVAITHGLKKFFSCRGIAIAVEYFWKLGNRNITVFVPQWRTRRDPNV
TEQHFLTQLQELGILSLTPARMVFGERIASHDDRFLLHLADKTGGIIVTN
DNFREFVNESVSWREIITKRLLQYTFVGDIFMVPDDPLGRSGPRLEEFLQ
KEVCLRDMQPLLSALPNVGMFDPSFRVPGTQAASTSHQPPTRIQGAPSSH
WLPQQPHFPLLPALPSLQQNLPMPAQRSSAETNELREALLKIFPDSEQRL
KIDQILVAHPYMKDLNALSAMVLD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU25227
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parent plasmid used DU24192