cDNA Clone SPAK (mouse)

• Synonyms: Stk39, AW227544, AW556857, DCHT, RF005, Rnl5
• Plasmid: pMK-RQ mSPAK Bam Not
• Parent Plasmid: pMK-RQ
• Genbank: NM_016866.2 (this sequence is optimised however)
• Species: Mouse

Sequence of Insert

GGATCCATGGCCGAGCCTAGCGGATCTCCCGTGCATGTGCAGCTGTCTCAGCAGGCCGCTCCTGTGACAGCCGCTGCTGCTACAGCTCCTGCCGCCGCTACATCTGCTCCAGCACCAGCTCCAGCCCCTGCTCCTGCTGCTTCTGCCGCCCCTGCACCCGCACCAGCTGCCGCTCCCGCTCCAGCTCCCGCAGCTCAGGCTGTGGGATGGCCCATCTGCAGAGATGCCTACGAGCTGCAGGAAGTGATCGGCAGCGGAGCCACAGCAGTGGTGCAGGCTGCCCTGTGCAAGCCCAGACAGGAACGGGTGGCCATCAAGCGGATCAACCTGGAAAAGTGCCAGACCAGCATGGACGAGCTGCTGAAAGAAATCCAGGCCATGAGCCAGTGCAGCCACCCCAACGTCGTGACCTACTACACCAGCTTCGTCGTGAAGGATGAGCTGTGGCTCGTGATGAAGCTGCTGAGCGGCGGCTCTATGCTGGACATCATCAAGTACATCGTGAACCGGGGCGAGCACAAGAACGGCGTGCTGGAAGAGGCCATCATTGCCACCATCCTGAAAGAGGTGCTGGAAGGCCTGGACTACCTGCACCGGAACGGCCAGATCCACAGGGATCTGAAGGCCGGCAACATCCTGCTGGGCGAGGATGGCTCTGTGCAGATCGCCGATTTTGGCGTGTCCGCCTTCCTGGCCACTGGCGGCGACGTGACCAGAAACAAAGTGCGCAAGACCTTCGTGGGCACCCCCTGCTGGATGGCCCCCGAAGTGATGGAACAAGTGCGGGGCTACGACTTCAAGGCCGACATGTGGTCCTTCGGCATCACCGCCATCGAGCTGGCTACAGGCGCCGCTCCCTACCACAAGTACCCCCCCATGAAGGTGCTGATGCTGACCCTGCAGAACGACCCCCCCACCCTGGAAACCGGCGTGGAGGACAAAGAAATGATGAAGAAGTACGGCAAGAGCTTCCGCAAGCTGCTGTCCCTGTGCCTGCAGAAAGATCCCAGCAAGAGGCCCACAGCCGCCGAACTGCTGAAGTGCAAGTTCTTCCAGAAGGCCAAGAACCGCGAGTACCTGATCGAGAAACTGCTGACCCGGACCCCCGATATCGCCCAGCGGGCTAAGAAAGTGCGGAGAGTGCCTGGCAGCTCCGGACATCTGCACAAGACCGAGGACGGCGACTGGGAGTGGAGCGACGACGAGATGGACGAGAAGTCCGAGGAAGGCAAGGCCGCTGCCAGCCAGGAAAAGTCTCGGCGCGTGAAAGAGGAAAACAGCGAGATCAGCGTGAACGCTGGCGGCATCCCCGAGCAGATCCAGAGCCTGAGCGTGCACGATAGCCAGGCCCAGCCCAACGCCAACGAGGACTACAGAGAAGGCCCCTGCGCCGTGAACCTGGTGCTGAGACTGCGGAACAGCCGGAAAGAGCTGAACGACATCAGATTCGAGTTCACCCCCGGCAGAGACACCGCTGATGGGGTGTCCCAGGAACTGTTCAGCGCCGGACTGGTGGACGGCCACGATGTCGTGATTGTGGCCGCCAATCTGCAGAAAATCGTGGACGACCCCAAGGCCCTGAAAACCCTGACCTTCAAGCTGGCCTCCGGCTGCGACGGCTCCGAGATCCCTGATGAAGTGAAGCTGATCGGCTTCGCCCAGCTGAGCGTGTCCTGAGCGGCCGC

Amino Acid Sequence

MAEPSGSPVHVQLSQQAAPVTAAAATAPAAATSAPAPAPAPAPAASAAPA
PAPAAAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQER
VAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVM
KLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQ
IHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCW
MAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQN
DPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQ
KAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDE
MDEKSEEGKAAASQEKSRRVKEENSEISVNAGGIPEQIQSLSVHDSQAQP
NANEDYREGPCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSA
GLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGF
AQLSVS*

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU48035
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Optimised SPAK mouse sequence to minimise issues with propagation in bacteria.; ; All SPAK plasmids MUST be grown at 30 degrees C or less to prevent recombination.