cDNA Clone SHARPIN

• Synonyms: SHANK-associated RH domain interactor (SHARPIN), SIPL1
• Plasmid: pSc-B-Sharpin E173-D320
• Parent Plasmid: pSC-B
• Genbank: AAH25244.1 NM_030974.4 → NP_112236.3
• Species: Human

Sequence of Insert

CATATGGAAGAGCTGGCAGGGAGCCTGGCCCGGGCTATTGCAGGTGGAGACGAGAAGGGGGCAGCCCAAGTGGCAGCCGTCCTGGCCCAGCATCGTGTGGCCCTGAGTGTTCAGCTTCAGGAGGCCTGCTTCCCACCTGGCCCCATCAGGCTGCAGGTCACACTTGAAGACGCTGCCTCTGCCGCATCCGCCGCGTCCTCTGCACACGTTGCCCTGCAGGTCCACCCCCACTGCACTGTTGCAGCTCTCCAGGAGCAGGTGTTCTCAGAGCTCGGTTTCCCGCCAGCCGTGCAACGCTGGGTCATCGGACGGTGCCTGTGTGTGCCTGAGCGCAGCCTTGCCTCTTACGGGGTTCGGCAGGATGGGGACCCTGCTTTCCTCTACTTGCTGTCAGCTCCCCGAGAAGCCCCAGCCACAGGACCTAGCCCTCAGCACCCCCAGAAGATGGACGGAAGGGCGAATTC

Amino Acid Sequence

EELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRL
QVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWV
IGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPATGPSPQHPQKMD

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU24884
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent change made at Pro303 (CCT > CCC) to knock out native Xho1 site at nt909. 1st round intermediate to producing Sharpin E173-S346 by primer extension; Transcript Variant 1 numbering
• Low Temp: No