cDNA Clone SHARPIN

• Synonyms: SHANK-associated RH domain interactor (SHARPIN), SIPL1
• Plasmid: pCDFDuet-1-6His-HOIP- UBA-RBR domain C885A/Sharpin E173-S346
• Parent Plasmid: pCDFDUET-1
• Expressed: Sharpin E173-S346
• Genbank: AAH25244.1 NM_030974.4 → NP_112236.3
• Species: Human

Sequence of Insert

CATATGGAAGAGCTGGCAGGGAGCCTGGCCCGGGCTATTGCAGGTGGAGACGAGAAGGGGGCAGCCCAAGTGGCAGCCGTCCTGGCCCAGCATCGTGTGGCCCTGAGTGTTCAGCTTCAGGAGGCCTGCTTCCCACCTGGCCCCATCAGGCTGCAGGTCACACTTGAAGACGCTGCCTCTGCCGCATCCGCCGCGTCCTCTGCACACGTTGCCCTGCAGGTCCACCCCCACTGCACTGTTGCAGCTCTCCAGGAGCAGGTGTTCTCAGAGCTCGGTTTCCCGCCAGCCGTGCAACGCTGGGTCATCGGACGGTGCCTGTGTGTGCCTGAGCGCAGCCTTGCCTCTTACGGGGTTCGGCAGGATGGGGACCCTGCTTTCCTCTACTTGCTGTCAGCTCCCCGAGAAGCCCCAGCCACAGGACCTAGCCCTCAGCACCCCCAGAAGATGGACGGGGAACTTGGACGCTTGTTTCCCCCATCATTGGGGCTACCCCCAGGCCCCCAGCCAGCTGCCTCCAGCCTGCCCAGTTAGCTCGAG

Amino Acid Sequence

MEELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRL
QVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWV
IGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPATGPSPQHPQKMDG
ELGRLFPPSLGLPPGPQPAASSLPS*

• Vector Type: Bacterial
• Antibiotic: Spectinomycin
• DU Number: DU24938
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent change made at Pro303 (CCT > CCC) to knock out native Xho1 site at nt909.; Transcript variant 1
• Low Temp: No