cDNA Clone USP35

• Plasmid: pGEX6P1 TEV USP35 604-1018
• Parent Plasmid: pGEX 6P-1
• Expressed: GST-TEV-USP35 604-1018
• Tag: GST

Sequence of Insert

GGATCCGAAAACCTGTATTTTCAGATGGAGAGATGCAGAAGGCGGAGACTGGGCAGCGTGATGCGGCCCACCGAGGACATCACCGCCAGAGAGCTGCCTCCCCCAACAAGCGCCCAGGGACCTGGCAGAGTGGGCCCCAGACGGCAGCGGAAGCACTGCATCACCGAGGATACCCCCCCCACCAGCCTGTACATCGAGGGCCTGGACAGCAAAGAGGCTGGCGGCCAGAGCAGCCAGGAAGAGAGAATCGAGCGCGAGGAAGAGGGCAAAGAGGAACGCACCGAGAAAGAGGAAGTCGGCGAGGAAGAAGAGAGCACCAGAGGCGAGGGCGAGCGCGAAAAAGAGGAAGAAGTCGAAGAGGAAGAGGAAAAGGTCGAGAAAGAGACAGAGAAAGAGGCCGAGCAGGAAAAAGAAGAGGACTCCCTGGGAGCCGGCACCCACCCCGATGCCGCCATTCCTAGCGGCGAGAGAACCTGCGGCAGCGAGGGCAGCAGAAGCGTGCTGGACCTGGTCAACTACTTCCTGTCCCCCGAGAAGCTGACCGCCGAGAACCGGTACTACTGCGAGAGCTGCGCCAGCCTGCAGGACGCCGAGAAGGTGGTGGAACTGAGCCAGGGCCCCTGCTACCTGATCCTGACCCTGCTGAGATTCAGCTTCGACCTGCGGACCATGCGGCGGAGAAAGATTCTGGACGACGTGTCCATCCCCCTGCTGCTGAGGCTGCCTCTGGCTGGCGGAAGAGGCCAGGCCTACGACCTGTGCAGCGTGGTGGTGCACAGCGGCGTGTCCAGCGAGAGCGGCCACTACTACTGCTACGCCAGAGAGGGCGCTGCCAGACCAGCCGCCTCTCTGGGCACAGCCGATAGACCCGAGCCCGAGAACCAGTGGTATCTGTTCAACGACACCAGAGTGTCCTTCAGCAGCTTCGAGAGCGTGTCCAACGTGACCTCATTCTTCCCAAAGGACACCGCCTACGTGCTGTTCTACCGGCAGCGGCCTAGAGAGGGCCCTGAGGCCGAACTGGGCAGCTCCAGAGTGCGGACCGAGCCCACCCTGCACAAGGACCTGATGGAAGCTATCTCCAAGGACAACATCCTGTATCTGCAGGAACAGGAAAAAGAGGCCAGAAGCAGAGCCGCCTACATCAGCGCCCTGCCCACCAGCCCTCACTGGGGCAGAGGCTTCGACGAGGACAAGGATGAGGACGAGGGCAGCCCTGGCGGCTGCAACCCTGCTGGCGGCAATGGCGGCGACTTCCACCGGCTGGTGTTCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSENLYFQMERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHPDAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQDAEKVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGGRGQAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPENQWYLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGSSRVRTEPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGRGFDEDKDEDEGSPGGCNPAGGNGGDFHRLVF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU55406
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please clone USP35 604-1018 with an N-terminal GST tag and a BLUNT TEV cleavage site. Thank you.
• Low Temp: No