cDNA Clone SPHK2

• Plasmid: pCMV5 FLAG1 SPHK2
• Parent Plasmid: pCMV5 FLAG1
• Expressed: Flag-SPHK2
• Genbank: NM_020126.3
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGAATGGACACCTTGAAGCAGAGGAGCAGCAGGACCAGAGGCC AGACCAGGAGCTGACCGGGAGCTGGGGCCACGGGCCTAGGAGCACCCTGG TCAGGGCTAAGGCCATGGCCCCGCCCCCACCGCCACTGGCTGCCAGCACC CCGCTCCTCCATGGCGAGTTTGGCTCCTACCCAGCCCGAGGCCCACGCTT TGCCCTCACCCTTACATCGCAGGCCCTGCACATACAGCGGCTGCGCCCCA AACCTGAAGCCAGGCCCCGGGGTGGCCTGGTCCCGTTGGCCGAGGTCTCA GGCTGCTGCACCCTGCGAAGCCGCAGCCCCTCAGACTCAGCGGCCTACTT CTGCATCTACACCTACCCTCGGGGCCGGCGCGGGGCCCGGCGCAGAGCCA CTCGCACCTTCCGGGCAGATGGGGCCGCCACCTACGAAGAGAACCGTGCC GAGGCCCAGCGCTGGGCCACTGCCCTCACCTGTCTGCTCCGAGGACTGCC ACTGCCCGGGGATGGGGAGATCACCCCTGACCTGCTACCTCGGCCGCCCC GGTTGCTTCTATTGGTCAATCCCTTTGGGGGTCGGGGCCTGGCCTGGCAG TGGTGTAAGAACCACGTGCTTCCCATGATCTCTGAAGCTGGGCTGTCCTT CAACCTCATCCAGACAGAACGACAGAACCACGCCCGGGAGCTGGTCCAGG GGCTGAGCCTGAGTGAGTGGGATGGCATCGTCACGGTCTCGGGAGACGGG CTGCTCCATGAGGTGCTGAACGGGCTCCTAGATCGCCCTGACTGGGAGGA AGCTGTGAAGATGCCTGTGGGCATCCTCCCCTGCGGCTCGGGCAACGCGC TGGCCGGAGCAGTGAACCAGCACGGGGGATTTGAGCCAGCCCTGGGCCTC GACCTGTTGCTCAACTGCTCACTGTTGCTGTGCCGGGGTGGTGGCCACCC ACTGGACCTGCTCTCCGTGACGCTGGCCTCGGGCTCCCGCTGTTTCTCCT TCCTGTCTGTGGCCTGGGGCTTCGTGTCAGATGTGGATATCCAGAGCGAG CGCTTCAGGGCCTTGGGCAGTGCCCGCTTCACACTGGGCACGGTGCTGGG CCTCGCCACACTGCACACCTACCGCGGACGCCTCTCCTACCTCCCCGCCA CTGTGGAACCTGCCTCGCCCACCCCTGCCCATAGCCTGCCTCGTGCCAAG TCGGAGCTGACCCTAACCCCAGACCCAGCCCCGCCCATGGCCCACTCACC CCTGCATCGTTCTGTGTCTGACCTGCCTCTTCCCCTGCCCCAGCCTGCCC TGGCCTCTCCTGGCTCGCCAGAACCCCTGCCCATCCTGTCCCTCAACGGT GGGGGCCCAGAGCTGGCTGGGGACTGGGGTGGGGCTGGGGATGCTCCGCT GTCCCCGGACCCACTGCTGTCTTCACCTCCTGGCTCTCCCAAGGCAGCTC TACACTCACCCGTCTCCGAAGGGGCCCCCGTAATTCCCCCATCCTCTGGG CTCCCACTTCCCACCCCTGATGCCCGGGTAGGGGCCTCCACCTGCGGCCC GCCCGACCACCTGCTGCCTCCGCTGGGCACCCCGCTGCCCCCAGACTGGG TGACGCTGGAGGGGGACTTTGTGCTCATGTTGGCCATCTCGCCCAGCCAC CTAGGCGCTGACCTGGTGGCAGCTCCGCATGCGCGCTTCGACGACGGCCT GGTGCACCTGTGCTGGGTGCGTAGCGGCATCTCGCGGGCTGCGCTGCTGC GCCTTTTCTTGGCCATGGAGCGTGGTAGCCACTTCAGCCTGGGCTGTCCG CAGCTGGGCTACGCCGCGGCCCGTGCCTTCCGCCTAGAGCCGCTCACACC ACGCGGCGTGCTCACAGTGGACGGGGAGCAGGTGGAGTATGGGCCGCTAC AGGCACAGATGCACCCTGGCATCGGTACACTGCTCACTGGGCCTCCTGGC TGCCCGGGGCGGGAGCCCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMNGHLEAEEQQDQRPDQELTGSWGHGPRSTLVRAKAMAPPPPPLAASTPLLHGEFGSYPARGPRFALTLTSQALHIQRLRPKPEARPRG
GLVPLAEVSGCCTLRSRSPSDSAAYFCIYTYPRGRRGARRRATRTFRADGAATYEENRAEAQRWATALTCLLRGLPLPGDGEITPDLLPRPPRLLLLVNP
FGGRGLAWQWCKNHVLPMISEAGLSFNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILPCGSGNALAGAVNQH
GGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPATVEPASPT
PAHSLPRAKSELTLTPDPAPPMAHSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGGPELAGDWGGAGDAPLSPDPLLSSPPGSPKAALHSPVSEG
APVIPPSSGLPLPTPDARVGASTCGPPDHLLPPLGTPLPPDWVTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLFLAMER
GSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLTGPPGCPGREP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU56201
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: SPHK2 from DU12551 into mammalian expression vector with N-terminal FLAG tag
• Low Temp: No