cDNA Clone NMNAT2

• Plasmid: pcDNA5D FRT/TO- NMNAT2 C-ThrLess-C-FLAG
• Parent Plasmid: pcDNA5D FRT/TO-C-FLAG
• Expressed: NMNAT2-FLAG Thr to Ala (10 C-terminal)
• Tag: FLAG
• Species: Human

Sequence of Insert

CTCGAGGCCGCCATGACCGAGACCACCAAGACCCACGTTATCTTGCTCGC
CTGCGGCAGCTTCAATCCCATCACCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAACTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACACCTGGCAGACGACCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGGCTGGCTGCAT
CCTCTCCAATGTCAACGCTCCTTCCATGGCTCCTGTGATCGGACAGCCAC
AAAACGAGGCCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCGCCAAG
CCCGCTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCGCCTTTGTAGATGAGAATG
CCAATCTGGGCGCGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACGCTGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAGCCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC

Amino Acid Sequence

MTETTKTHVILLACGSFNPITKGHIQMFERARDYLHKTGRFIVIGGIVSP
VHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWECYQDTWQTTCSVLE
HHRDLMKRVAGCILSNVNAPSMAPVIGQPQNEAPQPIYQNSNVAAKPAAA
KILGKVGESLSRICCVRPPVERFAFVDENANLGAVMRYEEIELRILLLCG
SDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADADRIMNHSSILRKY
KNNIMVVKDDINHPMSVVSSAKSRLALQHGDGHVVDYLSQPVIDYILKSQ
LYINASGAAADYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29083
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C-terminal 10 Thr residues mutated to Ala. Parental 41573
• Low Temp: No