cDNA Clone NMNAT2

• Plasmid: pcDNA5D FRT/TO- NMNAT2 ThrLess-C-FLAG
• Parent Plasmid: pcDNA5D FRT/TO-C-FLAG
• Expressed: NMNAT2-FLAG all Thr to Ala
• Tag: FLAG
• Species: Human

Sequence of Insert

CTCGAGGCCGCCATGGCCGAGGCCGCCAAGGCCCACGTTATCTTGCTCGC
CTGCGGCAGCTTCAATCCCATCGCCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAGCTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACGCCTGGCAGGCGGCCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGGCTGGCTGCAT
CCTCTCCAATGTCAACGCTCCTTCCATGGCTCCTGTGATCGGACAGCCAC
AAAACGAGGCCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCGCCAAG
CCCGCTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCGCCTTTGTAGATGAGAATG
CCAATCTGGGCGCGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACGCTGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAGCCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC

Amino Acid Sequence

MAEAAKAHVILLACGSFNPIAKGHIQMFERARDYLHKAGRFIVIGGIVSP
VHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWECYQDAWQAACSVLE
HHRDLMKRVAGCILSNVNAPSMAPVIGQPQNEAPQPIYQNSNVAAKPAAA
KILGKVGESLSRICCVRPPVERFAFVDENANLGAVMRYEEIELRILLLCG
SDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADADRIMNHSSILRKY
KNNIMVVKDDINHPMSVVSSAKSRLALQHGDGHVVDYLSQPVIDYILKSQ
LYINASGAAADYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29084
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: all 19 Thr residues mutated to Ala. Parental 41573
• Low Temp: No