cDNA Clone NMNAT2

• Plasmid: pET6His-SUMO-NMNAT2 (N9 Thr to Ala)
• Parent Plasmid: pET6His-SUMO
• Expressed: 6His-SUMO-NMNAT2 (N9 Thr to Ala)
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

CTCGAGGCCGCCATGGCCGAGGCCGCCAAGGCCCACGTTATCTTGCTCGC
CTGCGGCAGCTTCAATCCCATCGCCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAGCTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACGCCTGGCAGGCGGCCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGACTGGCTGCAT
CCTCTCCAATGTCAACACACCTTCCATGACACCTGTGATCGGACAGCCAC
AAAACGAGACCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCACCAAG
CCCACTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCACCTTTGTAGATGAGAATG
CCAATCTGGGCACGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACACAGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAACCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSGEFGVEAAMAEAAKAHVILLACGSFNPIAKGHIQMFERARDYLHK
AGRFIVIGGIVSPVHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWEC
YQDAWQAACSVLEHHRDLMKRVTGCILSNVNTPSMTPVIGQPQNETPQPI
YQNSNVATKPTAAKILGKVGESLSRICCVRPPVERFTFVDENANLGTVMR
YEEIELRILLLCGSDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADT
DRIMNHSSILRKYKNNIMVVKDDINHPMSVVSSTKSRLALQHGDGHVVDY
LSQPVIDYILKSQLYINASGAAA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29094
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-terminal 9 Thr residues mutated to Ala. 45322 parental. linker still to be deleted
• Low Temp: No