cDNA Clone NMNAT2

• Plasmid: pET6His-SUMO-NMNAT2 (C10 Thr to Ala)
• Parent Plasmid: pET6His-SUMO
• Expressed: 6His-SUMO-NMNAT2 (C10 Thr to Ala)
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

CTCGAGGCCGCCATGACCGAGACCACCAAGACCCACGTTATCTTGCTCGC
CTGCGGCAGCTTCAATCCCATCACCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAACTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACACCTGGCAGACGACCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGGCTGGCTGCAT
CCTCTCCAATGTCAACGCTCCTTCCATGGCTCCTGTGATCGGACAGCCAC
AAAACGAGGCCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCGCCAAG
CCCGCTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCGCCTTTGTAGATGAGAATG
CCAATCTGGGCGCGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACGCTGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAGCCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSGEFGVEAAMTETTKTHVILLACGSFNPITKGHIQMFERARDYLHK
TGRFIVIGGIVSPVHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWEC
YQDTWQTTCSVLEHHRDLMKRVAGCILSNVNAPSMAPVIGQPQNEAPQPI
YQNSNVAAKPAAAKILGKVGESLSRICCVRPPVERFAFVDENANLGAVMR
YEEIELRILLLCGSDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADA
DRIMNHSSILRKYKNNIMVVKDDINHPMSVVSSAKSRLALQHGDGHVVDY
LSQPVIDYILKSQLYINASGAAA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29095
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C-terminal 10 Thr residues mutated to Ala. Parental 45322. Linker still to be deleted
• Low Temp: No