cDNA Clone NMNAT2

• Plasmid: pET6His-SUMO-NMNAT2 (all Thr to Ala)
• Parent Plasmid: pET6His-SUMO
• Expressed: 6His-SUMO-NMNAT2 (all Thr to Ala)
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

CTCGAGGCCGCCATGGCCGAGGCCGCCAAGGCCCACGTTATCTTGCTCGC
CTGCGGCAGCTTCAATCCCATCGCCAAAGGGCACATTCAGATGTTTGAAA
GAGCCAGGGATTATCTGCACAAAGCTGGAAGGTTTATTGTGATTGGCGGG
ATTGTCTCCCCTGTCCACGACTCCTATGGAAAACAGGGCCTCGTGTCAAG
CCGGCACCGTCTCATCATGTGTCAGCTGGCCGTCCAGAATTCTGATTGGA
TCAGGGTGGACCCTTGGGAGTGCTACCAGGACGCCTGGCAGGCGGCCTGC
AGCGTGTTGGAACACCACCGGGACCTCATGAAGAGGGTGGCTGGCTGCAT
CCTCTCCAATGTCAACGCTCCTTCCATGGCTCCTGTGATCGGACAGCCAC
AAAACGAGGCCCCCCAGCCCATTTACCAGAACAGCAACGTGGCCGCCAAG
CCCGCTGCAGCCAAGATCTTGGGGAAGGTGGGAGAAAGCCTCAGCCGGAT
CTGCTGTGTCCGCCCGCCGGTGGAGCGTTTCGCCTTTGTAGATGAGAATG
CCAATCTGGGCGCGGTGATGCGGTATGAAGAGATTGAGCTACGGATCCTG
CTGCTGTGTGGTAGTGACCTGCTGGAGTCCTTCTGCATCCCAGGGCTCTG
GAACGAGGCAGATATGGAGGTGATTGTTGGTGACTTTGGGATTGTGGTGG
TGCCCCGGGATGCAGCCGACGCTGACCGAATCATGAATCACTCCTCAATA
CTCCGCAAATACAAAAACAACATCATGGTGGTGAAGGATGACATCAACCA
TCCCATGTCTGTTGTCAGCTCAGCCAAGAGCAGGCTGGCCCTGCAGCATG
GGGACGGCCATGTTGTGGATTACCTGTCCCAGCCGGTCATCGACTACATC
CTCAAAAGCCAGCTGTACATCAATGCCTCCGGCGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSGEFGVEAAMAEAAKAHVILLACGSFNPIAKGHIQMFERARDYLHK
AGRFIVIGGIVSPVHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWEC
YQDAWQAACSVLEHHRDLMKRVAGCILSNVNAPSMAPVIGQPQNEAPQPI
YQNSNVAAKPAAAKILGKVGESLSRICCVRPPVERFAFVDENANLGAVMR
YEEIELRILLLCGSDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADA
DRIMNHSSILRKYKNNIMVVKDDINHPMSVVSSAKSRLALQHGDGHVVDY
LSQPVIDYILKSQLYINASGAAA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29096
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: all 19 Thr residues mutated to Ala. Parental 45322. linker still to be deleted
• Low Temp: No