cDNA Clone PTPN14

• Plasmid: pCS2+-DU-HA-PTPN14 S594E
• Parent Plasmid: pCS2+-DU-HA
• Expressed: HA-PTPN14 S594E
• Tag: HA
• Species: Human

Sequence of Insert

agatctATGCCTTTTGGTCTGAAGCTCCGCCGGACACGGCGCTACAACGT
CCTGAGCAAGAACTGCTTTGTCACACGGATTCGCCTGCTGGACAGCAATG
TTATCGAGTGCACGCTGTCGGTGGAAAGCACAGGGCAAGAATGCCTGGAG
GCTGTGGCCCAGAGGCTGGAGCTGCGAGAGACGCACTACTTTGGCCTTTG
GTTTCTCAGCAAGAGCCAGCAAGCACGATGGGTGGAGCTGGAGAAACCTC
TGAAGAAACATCTGGACAAATTCGCTAATGAGCCTTTGCTTTTCTTTGGA
GTCATGTTCTATGTGCCAAATGTGTCATGGCTTCAGCAAGAGGCCACAAG
ATATCAGTATTACCTGCAAGTCAAAAAAGATGTGCTTGAAGGGCGATTAC
GATGTACATTGGACCAGGTGATTCGGCTAGCCGGCCTAGCTGTGCAAGCT
GATTTTGGAGACTATAATCAGTTTGATTCTCAAGATTTCCTCAGAGAGTA
TGTGCTATTTCCTATGGATTTGGCCCTGGAAGAGGCTGTTCTGGAGGAGC
TGACCCAGAAGGTAGCCCAAGAACACAAAGCCCACAGTGGAATCCTGCCA
GCAGAAGCTGAACTGATGTACATCAATGAAGTTGAACGTTTGGATGGATT
TGGACAGGAAATCTTCCCTGTAAAGGACAATCATGGAAACTGTGTACACC
TTGGCATTTTCTTTATGGGGATTTTCGTGAGGAACAGAATTGGAAGACAA
GCGGTAATATACAGGTGGAATGACATGGGGAATATCACTCATAACAAGTC
GACCATTCTAGTGGAGCTCATCAACAAAGAAGAGACTGCCCTCTTTCACA
CGGATGATATCGAAAATGCCAAGTATATTTCTCGGTTGTTTGCCACACGA
CACAAGTTTTACAAACAAAACAAAATCTGCACTGAACAGTCAAATTCTCC
ACCCCCCATCAGACGCCAGCCCACCTGGAGCCGGTCCTCTCTGCCCAGGC
AGCAGCCGTACATCCTGCCTCCCGTTCACGTCCAGTGTGGTGAGCACTAC
TCGGAAACGCACACCTCGCAAGACAGCATTTTTCATGGGAATGAAGAAGC
CTTGTATTGCAACTCTCACAACAGCCTGGACTTAAATTATTTAAATGGCA
CTGTCACCAATGGCAGCGTGTGTAGCGTTCACAGCGTCAACTCCCTCAAC
TGCTCGCAAAGTTTCATCCAGGCCTCCCCTGTATCCTCCAACCTCAGTAT
CCCTGGGAGTGACATCATGCGGGCCGACTACATCCCGAGCCACCGGCACA
GCGCGATCATCGTGCCCTCGTACAGGCCAACCCCCGATTATGAGACAGTC
ATGCGCCAGATGAAGAGGGGGATCCTGCATACAGACAGCCAGAGCCAGTC
TCTGAGAAACCTCAACATTATCAACACCCATGCCTACAACCAGCCAGAGG
ATCTGGTGTACAGCCAACCGGAGATGCGGGAGAGGCACCCCTACACTGTC
CCTTATGGGCCACAGGGGGTCTACAGCAACAAACTTGTCAGTCCATCTGA
CCAGAGGAACCCAAAGAATAATGTGGTACCAAGCAAGCCGGGGGCAAGCG
CCATCTCGCACACGGTGAGCACCCCAGAGCTGGCCAACATGCAGCTGCAG
GGCAGCCATAACTACAGCACGGCCCACATGCTTAAGAACTATCTCTTCAG
GCCACCGCCCCCCTACCCACGGCCACGACCTGCCACCAGCACCCCAGACC
TGGCCAGCCACCGCCACAAGTACGTCAGCGGCAGCgaaCCGGACCTGGTG
ACCCGGAAGGTGCAGCTCTCGGTGAAGACCTTCCAAGAGGACAGCTCTCC
GGTGGTTCATCAGTCTCTCCAGGAGGTGAGCGAGCCCCTCACGGCCACCA
AGCACCACGGCACTGTGAACAAGCGCCACAGCCTGGAGGTGATGAACAGC
ATGGTGCGGGGCATGGAGGCCATGACGCTCAAGTCGCTCCACCTCCCCAT
GGCTCGCCGCAACACGCTCCGGGAGCAGGGACCGCCCGAGGAGGGGTCAG
GCAGCCACGAGGTCCCCCAGCTCCCTCAGTATCACCACAAGAAGACCTTC
TCTGATGCCACTATGCTAATCCACAGCAGCGAGAGTGAGGAGGAGGAGGA
GGAGGCTCCAGAATCGGTGCCCCAGATCCCCATGCTCCGGGAGAAGATGG
AGTACAGTGCCCAGCTGCAGGCGGCCCTGGCCCGCATCCCCAACAAGCCC
CCGCCTGAGTACCCCGGTCCAAGGAAGAGTGTGAGCAATGGGGCTCTGAG
GCAGGACCAAGCCAGCCTTCCTCCCGCCATGGCCAGAGCCAGGGTGCTGA
GGCATGGGCCAGCCAAGGCCATCAGCATGTCTCGGACTGACCCGCCGGCT
GTCAACGGGGCCTCTCTCGGCCCATCCATCTCGGAACCCGACCTGACTAG
TGTGAAGGAGCGGGTCAAAAAAGAGCCTGTGAAGGAGAGACCTGTGTCTG
AAATGTTTTCCCTGGAAGACAGCATTATAGAGAGAGAGATGATGATCAGG
AATCTAGAGAAGCAGAAGATGGCAGGCCTGGAGGCACAGAAGAGGCCGCT
GATGTTGGCAGCATTGAATGGGCTCTCGGTGGCTCGAGTCTCAGGGCGGG
AAGAGAATCGAGTTGATGCCACCCGGGTTCCCATGGACGAGAGGTTCAGA
ACCCTGAAGAAGAAACTAGAAGAGGGAATGGTGTTCACAGAATATGAGCA
AATTCCAAAGAAAAAGGCGAATGGCATTTTCAGCACAGCAGCTCTGCCAG
AAAACGCCGAGCGCAGCCGAATCCGTGAAGTTGTCCCCTATGAGGAGAAT
CGAGTAGAGCTGATACCAACCAAAGAAAATAACACAGGATACATTAATGC
CTCCCACATCAAGGTGGTGGTTGGCGGGGCAGAATGGCACTACATAGCCA
CCCAGGGGCCCCTGCCACACACGTGCCACGACTTCTGGCAGATGGTGTGG
GAGCAGGGAGTGAATGTGATTGCCATGGTCACTGCAGAGGAGGAGGGTGG
ACGAACCAAAAGCCACCGATACTGGCCCAAACTAGGTTCAAAGCACAGCT
CAGCCACCTATGGCAAGTTCAAGGTCACCACGAAGTTTCGAACGGATTCT
GTTTGCTATGCAACCACGGGCTTGAAGGTCAAGCACCTTTTGTCTGGGCA
AGAAAGGACGGTGTGGCATTTACAATATACTGACTGGCCAGATCACGGCT
GTCCAGAGGATGTCCAAGGATTTTTATCCTACTTGGAGGAGATCCAGTCG
GTCCGTCGCCATACCAACAGCATGCTGGAAGGCACCAAGAACCGGCACCC
GCCCATCGTGGTCCACTGTAGTGCTGGGGTGGGAAGGACCGGCGTGCTCA
TTCTTTCTGAGCTGATGATCTACTGCTTGGAACATAACGAAAAGGTGGAA
GTGCCCATGATGCTGAGGCTCCTCAGGGAGCAGAGGATGTTCATGATCCA
GACTATCGCTCAGTACAAGTTTGTCTACCAAGTCCTCATCCAGTTCCTCC
AAAACTCCAGACTCATTTAAgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMPFGLKLRRTRRYNVLSKNCFVTRIRLLDSNVIECTLS
VESTGQECLEAVAQRLELRETHYFGLWFLSKSQQARWVELEKPLKKHLDK
FANEPLLFFGVMFYVPNVSWLQQEATRYQYYLQVKKDVLEGRLRCTLDQV
IRLAGLAVQADFGDYNQFDSQDFLREYVLFPMDLALEEAVLEELTQKVAQ
EHKAHSGILPAEAELMYINEVERLDGFGQEIFPVKDNHGNCVHLGIFFMG
IFVRNRIGRQAVIYRWNDMGNITHNKSTILVELINKEETALFHTDDIENA
KYISRLFATRHKFYKQNKICTEQSNSPPPIRRQPTWSRSSLPRQQPYILP
PVHVQCGEHYSETHTSQDSIFHGNEEALYCNSHNSLDLNYLNGTVTNGSV
CSVHSVNSLNCSQSFIQASPVSSNLSIPGSDIMRADYIPSHRHSAIIVPS
YRPTPDYETVMRQMKRGILHTDSQSQSLRNLNIINTHAYNQPEDLVYSQP
EMRERHPYTVPYGPQGVYSNKLVSPSDQRNPKNNVVPSKPGASAISHTVS
TPELANMQLQGSHNYSTAHMLKNYLFRPPPPYPRPRPATSTPDLASHRHK
YVSGSEPDLVTRKVQLSVKTFQEDSSPVVHQSLQEVSEPLTATKHHGTVN
KRHSLEVMNSMVRGMEAMTLKSLHLPMARRNTLREQGPPEEGSGSHEVPQ
LPQYHHKKTFSDATMLIHSSESEEEEEEAPESVPQIPMLREKMEYSAQLQ
AALARIPNKPPPEYPGPRKSVSNGALRQDQASLPPAMARARVLRHGPAKA
ISMSRTDPPAVNGASLGPSISEPDLTSVKERVKKEPVKERPVSEMFSLED
SIIEREMMIRNLEKQKMAGLEAQKRPLMLAALNGLSVARVSGREENRVDA
TRVPMDERFRTLKKKLEEGMVFTEYEQIPKKKANGIFSTAALPENAERSR
IREVVPYEENRVELIPTKENNTGYINASHIKVVVGGAEWHYIATQGPLPH
TCHDFWQMVWEQGVNVIAMVTAEEEGGRTKSHRYWPKLGSKHSSATYGKF
KVTTKFRTDSVCYATTGLKVKHLLSGQERTVWHLQYTDWPDHGCPEDVQG
FLSYLEEIQSVRRHTNSMLEGTKNRHPPIVVHCSAGVGRTGVLILSELMI
YCLEHNEKVEVPMMLRLLREQRMFMIQTIAQYKFVYQVLIQFLQNSRLI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29217
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 28162
• Low Temp: No