cDNA Clone DTX3

• Synonyms: x
• Plasmid: pSc-B-DTX3 isoform B
• Parent Plasmid: pSC-B
• Genbank: NM_001286245.1 → NP_001273174.1
• Species: Human

Sequence of Insert

ggatccATGCCAATTCTAAGCTCTTCAGGATCAAAAATGGCAGCCTGTGG
AGGCACCTGCAAGAACAAAGTGACTGTGTCCAAGCCCGTGTGGGACTTCC
TGAGCAAAGAGACCCCAGCCCGGCTGGCCCGGCTTCGGGAGGAGCACCGT
GTGTCCATCCTCATAGATGGCGAGACTTCTGACATCTATGTTCTCCAGCT
TTCCCCACAGGGTCCTCCCCCGGCCCCTCCAAATGGGCTCTACCTAGCCC
GGAAGGCTCTCAAGGGGCTGCTAAAAGAGGCAGAGAAAGAGCTGAAGAAA
GCTCAGAGGCAGGGGGAGCTGATGGGCTGCCTGGCTCTGGGGGGTGGAGG
GGAGCACCCTGAGATGCACCGCGCAGGCCCACCCCCTCTCCGAGCAGCCC
CACTTCTGCCCCCAGGAGCTCGGGGGCTCCCCCCTCCTCCTCCCCCCCTG
CCCCCACCTCTTCCTCCTCGCCTTCGGGAGGAGGCAGAAGAGCAGGAGAG
CACCTGCCCCATCTGTCTGGGGGAGATCCAGAATGCCAAGACATTGGAGA
AGTGCCGGCATTCATTCTGCGAGGGCTGCATCACCCGGGCTCTGCAGGTG
AAAAAGGCCTGCCCCATGTGCGGCCGCTTCTATGGGCAGCTGGTGGGCAA
CCAGCCCCAGAATGGGCGGATGCTGGTCTCTAAGGACGCCACCCTCCTAC
TGCCCAGCTATGAGAAGTACGGCACCATTGTCATCCAGTACGTCTTCCCG
CCCGGTGTCCAGGGGGCTGAACACCCAAACCCAGGAGTTCGGTATCCTGG
CACCACACGGGTGGCCTACCTCCCGGACTGCCCTGAGGGCAACAAGGTGC
TGACCCTGTTCCGCAAGGCGTTTGACCAGCGTCTCACCTTCACTATCGGC
ACGTCCATGACCACAGGGAGACCGAATGTCATCACCTGGAACGACATCCA
CCACAAGACCAGCTGCACAGGGGGACCCCAGCTGTTTGGGTACCCAGACC
CCACCTACCTGACCCGGGTGCAAGAGGAGCTGAGAGCGAAGGGTATCACA
GATGACTGAgcggccgc

Amino Acid Sequence

MPILSSSGSKMAACGGTCKNKVTVSKPVWDFLSKETPARLARLREEHRVS
ILIDGETSDIYVLQLSPQGPPPAPPNGLYLARKALKGLLKEAEKELKKAQ
RQGELMGCLALGGGGEHPEMHRAGPPPLRAAPLLPPGARGLPPPPPPLPP
PLPPRLREEAEEQESTCPICLGEIQNAKTLEKCRHSFCEGCITRALQVKK
ACPMCGRFYGQLVGNQPQNGRMLVSKDATLLLPSYEKYGTIVIQYVFPPG
VQGAEHPNPGVRYPGTTRVAYLPDCPEGNKVLTLFRKAFDQRLTFTIGTS
MTTGRPNVITWNDIHHKTSCTGGPQLFGYPDPTYLTRVQEELRAKGITDD
*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU29258
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform B = longest isoform; amplified product flanked BamHI-Not1 but internal Not1 discovered site at nt615. Can be excised for subcloning with BamHI-Sal1 (using 3' Sal1 site from pSc-B backbone)
• Low Temp: No