cDNA Clone mMBP

• Plasmid: pMA-8His-mMBP-3Ala

Sequence of Insert

AGATCTATGGGCCACCACCACCACCACCACCACCACAAGACCGAGGAGGGCAAGCTCGTCATCTGGATCAACGGCGACAAGGGCTACAACGGCCTCGCCGAGGTCGGCAAGAAGTTCGAGAAGGACACCGGCATCAAGGTCACCGTCGAGCACCCCGACAAGCTCGAGGAGAAGTTCCCCCAGGTCGCCGCCACCGGCGACGGCCCCGACATCATCTTCTGGGCCCACGACCGCTTCGGCGGCTACGCCCAGTCCGGCCTCCTCGCCGAGATCACCCCCGCCGCCGCCTTCCAGGACAAGCTCTACCCCTTCACCTGGGACGCCGTCCGCTACAACGGCAAGCTCATCGCCTACCCCATCGCCGTCGAGGCCCTCTCCCTCATCTACAACAAGGACCTCCTCCCCAACCCCCCCAAGACCTGGGAGGAGATCCCCGCCCTCGACAAGGAGCTCAAGGCCAAGGGCAAGTCCGCCCTCATGTTCAACCTCCAGGAGCCCTACTTCACCTGGCCCCTCATCGCCGCCGACGGCGGCTACGCCTTCAAGTACGCCGCCGGCAAGTACGACATCAAGGACGTCGGCGTCGACAACGCCGGCGCCAAGGCCGGCCTCACCTTCCTCGTCGACCTCATCAAGAACAAGCACATGAACGCCGACACCGACTACTCCATCGCCGAGCACGCCTTCAACCACGGCGAGACCGCCATGACCATCAACGGCCCCTGGGCCTGGTCCAACATCGACACCTCCGCCGTCAACTACGGCGTCACCGTCCTCCCCACCTTCAAGGGCCAGCCCTCCAAGCCCTTCGTCGGCGTCCTCTCCGCCGGCATCAACGCCGCCTCCCCCAACAAGGAGCTCGCCAAGGAGTTCCTCGAGAACTACCTCCTCACCGACGAGGGCCTCGAGGCCGTCAACAAGGACAAGCCCCTCGGCGCCGTCGCCCTCAAGTCCTACGAGGAGGAGCTCGTCAAGGACCCCCGCGTCGCCGCCACCATGGAGAACGCCCAGAAGGGCGAGATCATGCCCAACATCCCCCAGATGTCCGCCTTCTGGTACGCCGTCCGCACCGCCGTCATCAACGCCGCCTCCGGCCGCCAGACCGTCGACGCCGCCCTCGCCGCCGCCCAGACCAACGCCGCCGCCGGATCC

Amino Acid Sequence

MGHHHHHHHHKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHP
DKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPAAAFQDKLY
PFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELK
AKGKSALMFNLQEPYFTWPLIAADGGYAFKYAAGKYDIKDVGVDNAGAKA
GLTFLVDLIKNKHMNADTDYSIAEHAFNHGETAMTINGPWAWSNIDTSAV
NYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGL
EAVNKDKPLGAVALKSYEEELVKDPRVAATMENAQKGEIMPNIPQMSAFW
YAVRTAVINAASGRQTVDAALAAAQTNAAA

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU29543
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: modified MBP tag with increased solubility, affinity and crystallization properties (https://www.sciencedirect.com/science/article/pii/S1047847716300156?via%3Dihub )
• Low Temp: No