cDNA Clone MYCBP2

• Plasmid: pSc-B-MYCBP2 Met1-K2339 (fully corrected)
• Parent Plasmid: pSC-B
• Tag: Myc
• Species: Human

Sequence of Insert

GCTAGCATGCCGGTTCCCGACGGCTCCGTGGCTGCTGCGGGGCTGGGGCT
GGGGCTACCCGCCGCGGACTCCCGGGGTCACTACCAGCTGCTGCTGTCAG
GCCGGGCCCTGGCCGACCGCTACCGGAGGATTTATACCGCTGCGCTCAAT
GACAGGGACCAGGGGGGCGGCAGCGCTGGACACCCAGCCTCCAGGAATAA
GAAAATTTTAAATAAGAAGAAATTGAAAAGAAAACAGAAGAGCAAATCAA
AAGTGAAGACAAGAAGCAAGTCTGAAAACTTAGAGAATACAGTAATCATA
CCAGATATCAAACTACATAGCAATCCTTCTGCTTTCAATATTTACTGTAA
TGTACGCCATTGCGTTCTGGAATGGCAGAAGAAGGAAATATCATTGGCAG
CCGCATCTAAGAACTCTGTGCAGAGTGGAGAATCAGATAGTGATGAAGAA
GAGGAATCCAAAGAGCCCCCTATCAAGCTTCCAAAGATTATTGAGGTTGG
CCTTTGTGAAGTTTTTGAATTGATCAAAGAGACACGATTTTCTCATCCAT
CCCTGTGTCTCAGGAGTCTCCAAGCCCTGCTCAACGTGCTGCAGGGCCAG
CAGCCAGAAGGCCTCCAGTCTGAGCCACCTGAGGTCCTAGAGTCTCTCTT
CCAGCTTCTTTTGGAAATCACCGTTCGAAGTACTGGGATGAATGACAGCA
CAGGACAGTCCTTAACAGCACTTTCCTGTGCTTGCCTCTTTAGTCTGGTG
GCTTCTTGGGGAGAAACAGGAAGGACACTTCAGGCCATCTCTGCTATCCT
CACCAACAATGGAAGCCATGCTTGCCAAACTATTCAGGTGCCAACAATTC
TAAATTCGCTACAGAGAAGTGTACAAGCAGTTTTGGTGGGAAAAATTCAA
ATTCAGGACTGGTTTAGTAATGGCATTAAGAAAGCAGCTTTAATGCACAA
GTGGCCATTAAAAGAAATATCTGTTGATGAAGATGACCAATGTCTACTTC
AGAATGATGGATTTTTTCTTTATCTATTATGCAAGGATGGATTATATAAA
ATAGGCTCTGGATACAGTGGAACAGTTAGGGGCCATATATACAATTCTAC
ATCCCGTATTAGAAACAGAAAAGAAAAAAAGTCTTGGTTAGGGTATGCTC
AGGGTTATTTATTATATAGAGATGTGAATAACCACAGCATGACAGCCATA
AGGATAAGCCCTGAAACACTGGAGCAAGATGGTACTGTGATGTTACCAGA
TTGCCACACTGAAGGTCAAAATATTTTATTCACTGATGGAGAATATATTA
ATCAGATAGCTGCTTCAAGAGATGATGGCTTTGTTGTCAGAATATTTGCC
ACAAGCACTGAACCTGTTCTACAGCAAGAATTGCAACTTAAACTGGCTAG
AAAATGCTTACATGCCTGTGGTATCTCACTATTCGATCTGGAAAAGGACT
TGCATATTATAAGTACAGGATTTGATGAGGAGTCAGCAATTCTTGGTGCA
GGACGAGAGTTTGCGCTAATGAAAACAGCAAATGGAAAGATATATTACAC
TGGCAAATACCAGAGTCTTGGAATCAAACAAGGTGGTCCTTCAGCAGGAA
AATGGGTTGAGCTACCAATTACAAAATCTCCAAAGATAGTACACTTCTCA
GTTGGACACGATGGCTCTCACGCCCTTTTAGTTGCAGAAGATGGGAGCAT
ATTCTTTACAGGATCTGCTAGTAAAGGAGAAGATGGAGAATCAACTAAGA
GCAGACGGCAATCCAAACCTTATAAACCTAAAAAGATAATTAAGATGGAA
GGAAAGATTGTGGTATATACAGCCTGCAATAATGGAAGTAGTTCTGTTAT
TTCTAAAGATGGAGAACTCTACATGTTTGGAAAAGATGCCATTTACTCTG
ATAGTTCAAGTTTGGTAACTGATTTGAAGGGCCATTTTGTAACTCAGGTA
GCTATGGGCAAAGCTCACACTTGTGTTTTAATGAAGAATGGAGAGGTGTG
GACATTTGGTGTAAATAATAAAGGACAGTGTGGACGAGATACTGGTGCCA
TGAACCAAGGTGGGAAAGGGTTTGGAGTTGAAAATATGGCAACAGCAATG
GATGAAGACCTGGAAGAAGAACTAGATGAAAAAGATGAGAAGTCTATGAT
GTGCCCTCCAGGCATGCACAAATGGAAGCTGGAGCAGTGCATGGTTTGCA
CTGTCTGTGGAGACTGTACAGGTTATGGAGCCAGCTGTGTCAGTAGTGGA
CGGCCAGACAGAGTCCCCGGAGGGATCTGTGGTTGTGGTTCCGGAGAATC
TGGTTGTGCTGTGTGTGGATGTTGCAAGGCCTGTGCAAGAGAGTTAGATG
GTCAAGAGGCAAGACAAAGAGGAATTCTTGATGCAGTGAAAGAAATGATA
CCTTTAGATCTTCTTTTAGCTGTCCCAGTGCCCGGGGTTAACATTGAAGA
ACACCTTCAGTTACGACAAGAAGAAAAACGGCAACGTGTAATCAGAAGGC
ACAGATTAGAGGAAGGAAGAGGCCCCCTTGTATTTGCTGGTCCTATTTTT
ATGAACCATCGAGAACAGGCTCTAGCCAGACTCAGATCCCATCCAGCACA
GCTAAAGCATAAACGGGACAAGCACAAAGATGGAAGTGGAGAAAGAGGCG
AAAAGGATGCAAGCAAAATCACAACATACCCTCCAGGCTCTGTGCGATTT
GACTGTGAGCTCCGGGCAGTCCAAGTCAGCTGTGGATTTCACCATTCAGT
GGTTTTAATGGAAAATGGAGATGTCTATACATTTGGTTATGGGCAGCATG
GGCAGCTAGGACATGGAGATGTCAACTCCAGGGGATGTCCCACTCTTGTT
CAAGCATTGCCAGGCCCTAGCACACAAGTCACTGCAGGCAGCAACCATAC
GGCAGTACTTTTAATGGATGGACAGGTCTTCACATTTGGAAGTTTTTCTA
AAGGACAACTGGGCAGACCAATTTTGGATGTGCCATATTGGAATGCAAAG
CCAGCTCCCATGCCTAACATTGGATCAAAATATGGAAGAAAAGCTACTTG
GATAGGTGCAAGTGGGGACCAAACTTTTTTACGAATTGATGAAGCACTTA
TTAATTCTCATGTACTTGCTACATCAGAAATTTTTGCCAGTAAACACATA
ATAGGCTTGGTACCTGCTTCTATATCAGAACCTCCTCCATTTAAATGCCT
TCTGATAAATAAAGTGGATGGGAGTTGTAAAACTTTTAATGACTCAGAAC
AAGAGGATCTGCAAGGATTTGGTGTGTGTCTTGATCCTGTATACGATGTA
ATTTGGAGGTTTCGACCAAATACTAGAGAGCTGTGGTGTTACAATGCGGT
GGTTGCTGATGCCAGGCTTCCCTCTGCAGCAGACATGCAGTCCAGATGTA
GTATCCTAAGTCCTGAACTTGCCTTACCAACAGGATCAAGGGCCCTCACT
ACCCGATCTCATGCAGCTTTGCACATTTTAGGTTGTCTTGATACCTTGGC
AGCTATGCAGGACTTAAAAATGGGTGTTGCAAGTACAGAGGAAGAGACTC
AAGCAGTAATGAAGGTTTATTCTAAAGAAGATTATAGTGTGGTAAACAGG
TTTGAAAGTCATGGAGGAGGCTGGGGTTATTCTGCCCATTCAGTAGAAGC
TATACGTTTCAGTGCCGACACTGATATTTTACTTGGTGGTCTTGGTCTGT
TTGGAGGTAGAGGAGAATATACTGCTAAAATTAAGCTGTTTGAATTGGGT
CCTGATGGAGGAGATCATGAAACTGATGGTGACCTTCTTGCAGAGACTGA
TGTATTGGCTTATGACTGTGCTGCTAGAGAAAAATATGCAATGATGTTTG
ATGAGCCTGTTCTCCTGCAAGCTGGGTGGTGGTATGTGGCATGGGCCCGA
GTGTCAGGACCCAGCAGTGACTGTGGATCTCATGGACAGGCATCTATTAC
CACAGATGATGGGGTTGTTTTCCAGTTCAAGAGTTCAAAGAAATCAAATA
ATGGTACAGATGTTAATGCGGGTCAGATACCTCAGTTATTATACAGACTT
CCAACCAGTGATGGCAGTGCTTCAAAAGGCAAACAGCAAACCAGTGAACC
TGTACACATTTTAAAGAGGTCTTTTGCAAGAACTGTCTCAGTGGAATGTT
TTGAGTCATTGTTGAGTATTCTTCACTGGAGCTGGACCACCTTAGTCTTA
GGAGTTGAAGAACTTAGAGGATTAAAAGGATTCCAGTTCACAGCTACACT
CCTAGATTTAGAGAGACTGCGCTTTGTGGGTACCTGTTGTCTGAGGTTAT
TGCGTGTCTATACCTGTGAAATTTACCCAGTGTCAGCTACAGGAAAAGCA
GTTGTAGAAGAAACTAGCAAATTAGCAGAGTGTATTGGAAAAACCAGAAC
TTTGTTAAGAAAAATTTTATCAGAAGGAGTTGATCACTGCATGGTGAAAT
TGGATAATGATCCTCAAGGATATCTCAGTCAACCCTTGAGTCTTCTAGAA
GCTGTCCTTCAGGAATGTCATAATACTTTCACTGCCTGCTTTCATTCTTT
CTACCCAACTCCTGCCTTACAGTGGGCTTGCCTTTGTGATCTGCTGAATT
GTTTGGATCAGGATATCCAAGAAGCAAACTTCAAGACATCAAGTAGCCGA
CTCCTTGCAGCTGTTATGTCAGCTCTGTGTCACACGTCTGTTAAGCTGAC
TTCCATCTTCCCGATTGCGTATGATGGAGAAGTATTACTACGATCAATTG
TTAAACAAGTTAGTACAGAGAACGACTCAACACTAGTTCATCGTTTTCCC
CTTTTGGTGGCACATATGGAAAAACTCAGCCAGAGTGAAGAGAATATCTC
AGGGATGACAAGCTTCCGTGAAGTTCTGGAGAAAATGCTGGTCATTGTTG
TGCTACCAGTCAGGAACAGCCTGAGGAGAGAAAATGAACTCTTCTCCTCC
CACCTCGTCTCTAACACCTGTGGATTACTGGCCAGCATTGTCAGTGAACT
GACAGTGTCAGCCCTGGGATCTGAGGTTGATGGACTTAATTCTCTTCACT
CTGTAAAAGCTAGTGCTAACCGATTTACAAAAACAAGTCAGGGCAGAAGT
TGGAACACTGGGAACGGGTCCCCTGATGCAATCTGTTTTTCAGTAGACAA
ACCTGGAATAGTTGTGGTTGGTTTCTCTGTCTATGGAGGAGGTGGAATTC
ATGAATATGAATTAGAGGTGTTAGTTGATGATAGTGAACATGCAGGAGAT
TCAACTCATTCCCACAGATGGACATCTCTGGAATTAGTGAAAGGAACGTA
CACAACGGATGACTCACCCAGTGATATAGCTGAGATCAGACTTGACAAAG
TGGTTCCTTTAAAGGAAAATGTTAAATATGCTGTGCGCTTGAGGAACTAT
GGAAGCCGTACAGCCAATGGAGATGGAGGAATGACCACAGTTCAGTGCCC
TGATGGTGTGACATTCACATTCAGCACGTGCAGCTTGAGCAGTAACGGCA
CAAACCAAACCAGAGGACAGATCCCACAGATACTCTACTATAGGAGTGAA
TTTGATGGAGATTTACAATCCCAACTTCTGAGTAAAGCCAATGAAGAAGA
TAAAAACTGTAGCAGAGCATTGTCTGTTGTAAGCACTGTCGTTCGAGCCT
CTAAGGACCTCCTGCACAGAGCTCTTGCTGTGGATGCTGATGACATTCCA
GAACTGCTGAGTTCTTCCAGTCTGTTTTCCATGCTGCTCCCCCTTATTAT
AGCCTACATAGGACCAGTAGCTGCTGCTATTCCCAAGGTGGCTGTAGAAG
TCTTTGGCCTTGTCCAACAATTGCTTCCGTCAGTTGCCATTTTGAATCAG
AAGTATGCACCGCCTGCCTTCAACCCTAATCAGTCGACAGATAGCACCAC
AGGAAACCAGCCTGAACAGGGCCTCTCTGCTTGTACAACCTCCAGTCACT
ATGCTGTCATAGAGAGTGAGCACCCGTATAAACCTGCCTGTGTGATGCAT
TACAAGGTGACATTCCCAGAATGCGTGAGGTGGATGACAATCGAATTTGA
CCCTCAGTGTGGTACTGCACAGTCAGAAGATGTCCTTCGTTTGTTGATTC
CTGTCAGAACTGTTCAGAATTCAGGATATGGACCAAAATTGACATCTGTT
CATGAAAATCTTAATTCATGGATAGAATTAAAGAAATTTTCAGGATCCTC
TGGGTGGCCTACTATGGTTTTGGTGTTGCCAGGAAATGAGGCCCTTTTTT
CATTGGAGACTGCATCAGATTATGTGAAAGATGACAAAGCTTCTTTCTAT
GGTTTTAAGTGTTTTGCAATTGGATATGAATTTAGCCCTGGACCTGATGA
GGGAGTCATCCAATTGGAAAAAGAATTAGCCAATCTTGGTGGGGTTTGTG
CAGCAGCTCTGATGAAGAAGGACCTAGCACTTCCTATTGGTAATGAATTA
GAAGAAGACCTTGAAATTCTTGAGGAGGCTGCATTGCAGGTGTGCAAAAC
CCATTCTGGAATTCTTGGAAAGGGTCTAGCTCTTTCTCATTCACCAACTA
TATTAGAAGCACTTGAGGGAAATTTACCACTCCAAATCCAAAGCAATGAA
CAGTCTTTTCTGGATGATTTTATTGCCTGTGTCCCAGGATCAAGTGGTGG
AAGGCTTGCAAGGTGGCTTCAGCCAGATTCATATGCGGATCCTCAGAAAA
CATCTTTGATCCTGAATAAGGATGATATTCGTTGTGGTTGGCCTACCACC
ATAACTGTTCAAACAAAAGACCAGTATGGGGATGTGGTACATGTTCCCAA
TATGAAGGTGGAAGTGAAAGCTGTCCCTGTTTCTCAGAAAAAAATGTCTT
TACAACAAGATCAAGCAAAGAAACCTCAAAGGATTCCTGGCAGTCCTGCA
GTAACAGCTGCATCTTCTAATACTGACATGACTTATGGAGGGCTGGCATC
ACCAAAGCTAGATGTTTCATATGAACCAATGATAGTGAAGGAAGCTCGAT
ATATTGCCATAACAATGATGAAGCTCGAG

Amino Acid Sequence

MPVPDGSVAAAGLGLGLPAADSRGHYQLLLSGRALADRYRRIYTAALNDR
DQGGGSAGHPASRNKKILNKKKLKRKQKSKSKVKTRSKSENLENTVIIPD
IKLHSNPSAFNIYCNVRHCVLEWQKKEISLAAASKNSVQSGESDSDEEEE
SKEPPIKLPKIIEVGLCEVFELIKETRFSHPSLCLRSLQALLNVLQGQQP
EGLQSEPPEVLESLFQLLLEITVRSTGMNDSTGQSLTALSCACLFSLVAS
WGETGRTLQAISAILTNNGSHACQTIQVPTILNSLQRSVQAVLVGKIQIQ
DWFSNGIKKAALMHKWPLKEISVDEDDQCLLQNDGFFLYLLCKDGLYKIG
SGYSGTVRGHIYNSTSRIRNRKEKKSWLGYAQGYLLYRDVNNHSMTAIRI
SPETLEQDGTVMLPDCHTEGQNILFTDGEYINQIAASRDDGFVVRIFATS
TEPVLQQELQLKLARKCLHACGISLFDLEKDLHIISTGFDEESAILGAGR
EFALMKTANGKIYYTGKYQSLGIKQGGPSAGKWVELPITKSPKIVHFSVG
HDGSHALLVAEDGSIFFTGSASKGEDGESTKSRRQSKPYKPKKIIKMEGK
IVVYTACNNGSSSVISKDGELYMFGKDAIYSDSSSLVTDLKGHFVTQVAM
GKAHTCVLMKNGEVWTFGVNNKGQCGRDTGAMNQGGKGFGVENMATAMDE
DLEEELDEKDEKSMMCPPGMHKWKLEQCMVCTVCGDCTGYGASCVSSGRP
DRVPGGICGCGSGESGCAVCGCCKACARELDGQEARQRGILDAVKEMIPL
DLLLAVPVPGVNIEEHLQLRQEEKRQRVIRRHRLEEGRGPLVFAGPIFMN
HREQALARLRSHPAQLKHKRDKHKDGSGERGEKDASKITTYPPGSVRFDC
ELRAVQVSCGFHHSVVLMENGDVYTFGYGQHGQLGHGDVNSRGCPTLVQA
LPGPSTQVTAGSNHTAVLLMDGQVFTFGSFSKGQLGRPILDVPYWNAKPA
PMPNIGSKYGRKATWIGASGDQTFLRIDEALINSHVLATSEIFASKHIIG
LVPASISEPPPFKCLLINKVDGSCKTFNDSEQEDLQGFGVCLDPVYDVIW
RFRPNTRELWCYNAVVADARLPSAADMQSRCSILSPELALPTGSRALTTR
SHAALHILGCLDTLAAMQDLKMGVASTEEETQAVMKVYSKEDYSVVNRFE
SHGGGWGYSAHSVEAIRFSADTDILLGGLGLFGGRGEYTAKIKLFELGPD
GGDHETDGDLLAETDVLAYDCAAREKYAMMFDEPVLLQAGWWYVAWARVS
GPSSDCGSHGQASITTDDGVVFQFKSSKKSNNGTDVNAGQIPQLLYRLPT
SDGSASKGKQQTSEPVHILKRSFARTVSVECFESLLSILHWSWTTLVLGV
EELRGLKGFQFTATLLDLERLRFVGTCCLRLLRVYTCEIYPVSATGKAVV
EETSKLAECIGKTRTLLRKILSEGVDHCMVKLDNDPQGYLSQPLSLLEAV
LQECHNTFTACFHSFYPTPALQWACLCDLLNCLDQDIQEANFKTSSSRLL
AAVMSALCHTSVKLTSIFPIAYDGEVLLRSIVKQVSTENDSTLVHRFPLL
VAHMEKLSQSEENISGMTSFREVLEKMLVIVVLPVRNSLRRENELFSSHL
VSNTCGLLASIVSELTVSALGSEVDGLNSLHSVKASANRFTKTSQGRSWN
TGNGSPDAICFSVDKPGIVVVGFSVYGGGGIHEYELEVLVDDSEHAGDST
HSHRWTSLELVKGTYTTDDSPSDIAEIRLDKVVPLKENVKYAVRLRNYGS
RTANGDGGMTTVQCPDGVTFTFSTCSLSSNGTNQTRGQIPQILYYRSEFD
GDLQSQLLSKANEEDKNCSRALSVVSTVVRASKDLLHRALAVDADDIPEL
LSSSSLFSMLLPLIIAYIGPVAAAIPKVAVEVFGLVQQLLPSVAILNQKY
APPAFNPNQSTDSTTGNQPEQGLSACTTSSHYAVIESEHPYKPACVMHYK
VTFPECVRWMTIEFDPQCGTAQSEDVLRLLIPVRTVQNSGYGPKLTSVHE
NLNSWIELKKFSGSSGWPTMVLVLPGNEALFSLETASDYVKDDKASFYGF
KCFAIGYEFSPGPDEGVIQLEKELANLGGVCAAALMKKDLALPIGNELEE
DLEILEEAALQVCKTHSGILGKGLALSHSPTILEALEGNLPLQIQSNEQS
FLDDFIACVPGSSGGRLARWLQPDSYADPQKTSLILNKDDIRCGWPTTIT
VQTKDQYGDVVHVPNMKVEVKAVPVSQKKMSLQQDQAKKPQRIPGSPAVT
AASSNTDMTYGGLASPKLDVSYEPMIVKEARYIAITMMKLE

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU29671
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All non-silent mutations in precursors have been corrected restoring V323, M715, P993, M1280, V2268 (with respect to full-length Virdee group sequence), G839-K874 deletion now also successfully repaired.; ; Clone still contains the following changes relative to Virdee group ref sequence (Virdee MYCBP2 ref attached below):; Contains listed SNP A1667V - this SNP equates to A1705V in NM_015057.4; ; Silent mutations:; K125 (AAA > AAG), Y1096 (TAT > TAC), L1739 (TTG > TTA), C2006 (TGT > TGC).; There is an artificial Xho1 site at nt 6966 (to facilitate downstream joining of the two half lengths of full-length MYCBP2 now that all corrections to first half have been made).; outwith the sequences described this clone contains a 5' extension comprising overlap with FRT/TO-MYC Nhe1 and a 3' extension comprising overlap to FRT/TO-MYC Xho1 in DU58354 to join the two half lengths with NEB builder
• Low Temp: No