cDNA Clone MYCBP2

• Plasmid: pcDNA5-FRT/TO-MYCBP2 FL C4572A
• Parent Plasmid: pcDNA5-FRT/TO
• Expressed: MYCBP2 C4572A
• Genbank: NP_055872.4 minus first 38aa
• Species: Human

Amino Acid Sequence

MPVPDGSVAAAGLGLGLPAADSRGHYQLLLSGRALADRYRRIYTAALNDR
DQGGGSAGHPASRNKKILNKKKLKRKQKSKSKVKTRSKSENLENTVIIPD
IKLHSNPSAFNIYCNVRHCVLEWQKKEISLAAASKNSVQSGESDSDEEEE
SKEPPIKLPKIIEVGLCEVFELIKETRFSHPSLCLRSLQALLNVLQGQQP
EGLQSEPPEVLESLFQLLLEITVRSTGMNDSTGQSLTALSCACLFSLVAS
WGETGRTLQAISAILTNNGSHACQTIQVPTILNSLQRSVQAVLVGKIQIQ
DWFSNGIKKAALMHKWPLKEISVDEDDQCLLQNDGFFLYLLCKDGLYKIG
SGYSGTVRGHIYNSTSRIRNRKEKKSWLGYAQGYLLYRDVNNHSMTAIRI
SPETLEQDGTVMLPDCHTEGQNILFTDGEYINQIAASRDDGFVVRIFATS
TEPVLQQELQLKLARKCLHACGISLFDLEKDLHIISTGFDEESAILGAGR
EFALMKTANGKIYYTGKYQSLGIKQGGPSAGKWVELPITKSPKIVHFSVG
HDGSHALLVAEDGSIFFTGSASKGEDGESTKSRRQSKPYKPKKIIKMEGK
IVVYTACNNGSSSVISKDGELYMFGKDAIYSDSSSLVTDLKGHFVTQVAM
GKAHTCVLMKNGEVWTFGVNNKGQCGRDTGAMNQGGKGFGVENMATAMDE
DLEEELDEKDEKSMMCPPGMHKWKLEQCMVCTVCGDCTGYGASCVSSGRP
DRVPGGICGCGSGESGCAVCGCCKACARELDGQEARQRGILDAVKEMIPL
DLLLAVPVPGVNIEEHLQLRQEEKRQRVIRRHRLEEGRGPLVFAGPIFMN
HREQALARLRSHPAQLKHKRDKHKDGSGERGEKDASKITTYPPGSVRFDC
ELRAVQVSCGFHHSVVLMENGDVYTFGYGQHGQLGHGDVNSRGCPTLVQA
LPGPSTQVTAGSNHTAVLLMDGQVFTFGSFSKGQLGRPILDVPYWNAKPA
PMPNIGSKYGRKATWIGASGDQTFLRIDEALINSHVLATSEIFASKHIIG
LVPASISEPPPFKCLLINKVDGSCKTFNDSEQEDLQGFGVCLDPVYDVIW
RFRPNTRELWCYNAVVADARLPSAADMQSRCSILSPELALPTGSRALTTR
SHAALHILGCLDTLAAMQDLKMGVASTEEETQAVMKVYSKEDYSVVNRFE
SHGGGWGYSAHSVEAIRFSADTDILLGGLGLFGGRGEYTAKIKLFELGPD
GGDHETDGDLLAETDVLAYDCAAREKYAMMFDEPVLLQAGWWYVAWARVS
GPSSDCGSHGQASITTDDGVVFQFKSSKKSNNGTDVNAGQIPQLLYRLPT
SDGSASKGKQQTSEPVHILKRSFARTVSVECFESLLSILHWSWTTLVLGV
EELRGLKGFQFTATLLDLERLRFVGTCCLRLLRVYTCEIYPVSATGKAVV
EETSKLAECIGKTRTLLRKILSEGVDHCMVKLDNDPQGYLSQPLSLLEAV
LQECHNTFTACFHSFYPTPALQWACLCDLLNCLDQDIQEANFKTSSSRLL
AAVMSALCHTSVKLTSIFPIAYDGEVLLRSIVKQVSTENDSTLVHRFPLL
VAHMEKLSQSEENISGMTSFREVLEKMLVIVVLPVRNSLRRENELFSSHL
VSNTCGLLASIVSELTVSALGSEVDGLNSLHSVKASANRFTKTSQGRSWN
TGNGSPDAICFSVDKPGIVVVGFSVYGGGGIHEYELEVLVDDSEHAGDST
HSHRWTSLELVKGTYTTDDSPSDIAEIRLDKVVPLKENVKYAVRLRNYGS
RTANGDGGMTTVQCPDGVTFTFSTCSLSSNGTNQTRGQIPQILYYRSEFD
GDLQSQLLSKANEEDKNCSRALSVVSTVVRASKDLLHRALAVDADDIPEL
LSSSSLFSMLLPLIIAYIGPVAAAIPKVAVEVFGLVQQLLPSVAILNQKY
APPAFNPNQSTDSTTGNQPEQGLSACTTSSHYAVIESEHPYKPACVMHYK
VTFPECVRWMTIEFDPQCGTAQSEDVLRLLIPVRTVQNSGYGPKLTSVHE
NLNSWIELKKFSGSSGWPTMVLVLPGNEALFSLETASDYVKDDKASFYGF
KCFAIGYEFSPGPDEGVIQLEKELANLGGVCAAALMKKDLALPIGNELEE
DLEILEEAALQVCKTHSGILGKGLALSHSPTILEALEGNLPLQIQSNEQS
FLDDFIACVPGSSGGRLARWLQPDSYADPQKTSLILNKDDIRCGWPTTIT
VQTKDQYGDVVHVPNMKVEVKAVPVSQKKMSLQQDQAKKPQRIPGSPAVT
AASSNTDMTYGGLASPKLDVSYEPMIVKEARYIAITMMKVYENYSFEELR
FASPTPKRPSENMLIRVNNDGTYCANWTPGAIGLYTLHVTIDGIEIDAGL
EVKVKDPPKGMIPPGTQLVKPKSEPQPNKVRKFVAKDSAGLRIRSHPSLQ
SEQIGIVKVNGTITFIDEIHNDDGVWLRLNDETIKKYVPNMNGYTEAWCL
SFNQHLGKSLLVPVDESKTNTDDFFKDINSCCPQEATMQEQDMPFLRGGP
GMYKVVKTGPSGHNIRSCPNLRGIPIGMLVLGNKVKAVGEVTNSEGTWVQ
LDQNSMVEFCESDEGEAWSLARDRGGNQYLRHEDEQALLDQNSQTPPPSP
FSVQAFNKGASCSAQGFDYGLGNSKGDRGNISTSSKPASTSGKSELSSKH
SRSLKPDGRMSRTTADQKKPRGTESLSASESLILKSDAAKLRSDSHSRSL
SPNHNTLQTLKSDGRMPSSSRAESPGPGSRLSSPKPKTLPANRSSPSGAS
SPRSSSPHDKNLPQKSTAPVKTKLDPPRERSKSDSYTLDPDTLRKKKMPL
TEPLRGRSTSPKPKSVPKDSTDSPGSENRAPSPHVVQENLHSEVVEVCTS
STLKTNSLTDSTCDDSSEFKSVDEGSNKVHFSIGKAPLKDEQEMRASPKI
SRKCANRHTRPKKEKSSFLFKGDGSKPLEPAKQAMSPSVAECARAVFASF
LWHEGIVHDAMACSSFLKFHPELSKEHAPIRSSLNSQQPTEEKETKLKNR
HSLEISSALNMFNIAPHGPDISKMGSINKNKVLSMLKEPPLHEKCEDGKT
ETTFEMSMHNTMKSKSPLPLTLQHLVAFWEDISLATIKAASQNMIFPSPG
SCAVLKKKECEKENKKSKKEKKKKEKAEVRPRGNLFGEMAQLAVGGPEKD
TICELCGESHPYPVTYHMRQAHPGCGRYAGGQGYNSIGHFCGGWAGNCGD
GGIGGSTWYLVCDRCREKYLREKQAAAREKVKQSRRKPMQVKTPRALPTM
EAHQVIKANALFLLSLSSAAEPSILCYHPAKPFQSQLPSVKEGISEDLPV
KMPCLYLQTLARHHHENFVGYQDDNLFQDEMRYLRSTSVPAPYISVTPDA
SPNVFEEPESNMKSMPPSLETSPITDTDLAKRTVFQRSYSVVASEYDKQH
SILPARVKAIPRRRVNSGDTEVGSSLLRHPSPELSRLISAHSSLSKGERN
FQWPVLAFVIQHHDLEGLEIAMKQALRKSACRVFAMEAFNWLLCNVIQTT
SLHDILWHFVASLTPAPVEPEEEEDEENKTSKENSEQEKDTRVCEHPLSD
IVIAGEAAHPLPHTFHRLLQTISDLMMSLPSGSSLQQMALRCWSLKFKQS
DHQFLHQSNVFHHINNILSKSDDGDSEESFSISIQSGFEAMSQELCIVMC
LKDLTSIVDIKTSSRPAMIGSLTDGSTETFWESGDEDKNKTKNITINCVK
GINARYVSVHVDNSRDLGNKVTSMTFLTGKAVEDLCRIKQVDLDSRHIGW
VTSELPGGDNHIIKIELKGPENTLRVRQVKVLGWKDGESTKIAGQISASV
AQQRNCEAETLRVFRLITSQVFGKLISGDAEPTPEQEEKALLSSPEGEEK
VYNATSDADLKEHMVGIIFSRSKLTNLQKQVCAHIVQAIRMEATRVREEW
EHAISSKENANSQPNDEDASSDAYCFELLSMVLALSGSNVGRQYLAQQLT
LLQDLFSLLHTASPRVQRQVTSLLRRVLPEVTPSRLASIIGVKSLPPADI
SDIIHSTEKGDWNKLGILDMFLGCIAKALTVQLKAKGTTITGTAGTTVGK
GVTTVTLPMIFNSSYLRRGESHWWMKGSTPTQISEIIIKLIKDMAAGHLS
EAWSRVTKNAIAETIIALTKMEEEFRSPVRCIATTRLWLALASLCVLDQD
HVDRLSSGRWMGKDGQQKQMPMCDNHDDGETAAIILCNVCGNLCTDCDRF
LHLHRRTKTHQRQVFKEEEEAIKVDLHEGCGRTKLFWLMALADSKTMKAM
VEFREHTGKPTTSSSEACRFCGSRSGTELSAVGSVCSDADCQEYAKIACS
KTHPCGHPCGGVKNEEHCLPCLHGCDKSATSLKQDADDMCMICFTEALSA
APAIQLDCSHIFHLQCCRRVLENRWLGPRITFGFISCPICKNKINHIVLK
DLLDPIKELYEDVRRKALMRLEYEGLHKSEAITTPGVRFYNDPAGYAMNR
YAYYVCYKCRKAYFGGEARCDAEAGRGDDYDPRELICGACSDVSRAQMCP
KHGTDFLEYKCRYCCSVAVFFAFGTTHFCNACHDDFQRMTSIPKEELPHC
PAGPKGKQLEGTECPLHVVHPPTGEEFALGCGVCRNAHTF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65103
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GROW AT 30 DEGREES; untagged MYCBP2 full-length with the following changes relative to Virdee group ref sequence (Virdee MYCBP2 ref attached below):; Contains listed SNP A1667V - this SNP equates to A1705V in NM_015057.4. ; Silent mutations: K125 (AAA > AAG), Y1096 (TAT > TAC), L1739 (TTG > TTA), C2006 (TGT > TGC). ; ; 3' Not only, 5' site to be introduced
• Low Temp: No