cDNA Clone DAC

• Plasmid: pSc-B-MCS-TEV-DAC
• Parent Plasmid: pSC-B
• Genbank: xxxxx
• Tag: DAC

Sequence of Insert

catatgGGATCCGAATTCGAGCTCGTCGACAAGCTTGCGGCCGCACTCGA
GGAAAACCTGTATTTTCAGGGCATGTCTGCAATCCCGGGTGTACCGCAGA
TCGATGCGGAGTCCTACATCCTGATTGACTATAACTCCGGCAAAGTGCTC
GCCGAACAGAACGCAGATGTCCGCCGCGATCCTGCCAGCCTGACCAAAAT
GATGACCAGTTACGTTATCGGCCAGGCAATGAAAGCCGGTAAATTTAAAG
AAACTGATTTAGTCACTATCGGCAACGACGCATGGGCCACCGGTAACCCG
GTGTTTAAAGGTTCTTCGCTGATGTTCCTCAAACCGGGCATGCAGGTTCC
GGTTTCTCAGCTGATCCGCGGTATTAACCTGCAATCGGGTAACGATGCTT
GTGTCGCCATGGCCGATTTTGCCGCTGGTAGCCAGGACGCTTTTGTTGGC
TTGATGAACAGCTACGTTAACGCACTGGGCCTGAAAAATACCCACTTCCA
GACGGTACATGGTCTGGATGCTGATGGTCAGTACAGCTCCGCGCGAGATA
TGGCGCTGATCGGCCAGGCATTGATCCGTGACGTACCGAATGAATACTCG
ATCTATAAAGAAAAAGAATTTACGTTTAACGGTATTCGCCAGCTGAACCG
TAACGGCCTGTTATGGGATAACAGCCTGAATGTCGACGGCATCAAAACCG
GACACACTGACAAAGCAGGTTACAACCTTGTTGCTTCTGCGACTGAAGGC
CAGATGCGCTTGATTTCTGCGGTAATGGGCGGACGTACTTTTAAAGGCCG
TGAAGCCGAAAGTAAAAAACTGCTAACCTGGGGCTTCCGTTTCTTTGAAA
CCGTTAACCCAtgactcgag

Amino Acid Sequence

MGSEFELVDKLAAALEENLYFQGMSAIPGVPQIDAESYILIDYNSGKVLA
EQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPV
FKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGL
MNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSI
YKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQ
MRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNP*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU65337
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: intermediate for creating C-ter DAC tagging vector with TEV cleavage. polylinker sites upstream of tag and inframe with 5' Nde1 flanking site encoded ATG are Bam-Eco-Hind-Not (AAA frame)
• Low Temp: No