cDNA Clone RAB7A

• Synonyms: RAB7; CMT2B; PRO2706
• Plasmid: pCMV5D HA RAB7A Mito (MAO tag)
• Parent Plasmid: pCMV5D HA mcs Mito
• Expressed: HA RAB7A Mito (MAO tag)
• Genbank: NM_004637.5
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGACCTCTAGGAAGAAAGTGTTGCTGAAGGTTATCATCCTGGGAGATTCTGGAGTCGGGAAGACATCACTCATGAACCAGTATGTGAATAAGAAATTCAGCAATCAGTACAAAGCCACAATAGGAGCTGACTTTCTGACCAAGGAGGTGATGGTGGATGACAGGCTAGTCACAATGCAGATATGGGACACAGCAGGACAGGAACGGTTCCAGTCTCTCGGTGTGGCCTTCTACAGAGGTGCAGACTGCTGCGTTCTGGTATTTGATGTGACTGCCCCCAACACATTCAAAACCCTAGATAGCTGGAGAGATGAGTTTCTCATCCAGGCCAGTCCCCGAGATCCTGAAAACTTCCCATTTGTTGTGTTGGGAAACAAGATTGACCTCGAAAACAGACAAGTGGCCACAAAGCGGGCACAGGCCTGGTGCTACAGCAAAAACAACATTCCCTACTTTGAGACCAGTGCCAAGGAGGCCATCAACGTGGAGCAGGCGTTCCAGACGATTGCACGGAATGCACTTAAGCAGGAAACGGAGGTGGAGCTGTACAACGAATTTCCTGAACCTATCAAACTGGACAAGAATGACCGGGCCAAGGCCTCGGCAGAAAGCGGTGCTGGCGCCGGTGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMTSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVF
DVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPE
PIKLDKNDRAKASAESGAGAGAAAFWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5D HA RAB7A Mito (MAO tag)870.47 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61287
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: HA-Rab7A 1-204 (CSC residues removed at the C-terminus) fused to GAGAGAAA and a C terminal MAO fragment 489-end (FWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS).
• Low Temp: No