cDNA Clone RAB8B

• Plasmid: pCMV5D HA RAB8B Mito (MAO tag)
• Parent Plasmid: pCMV5D HA mcs Mito
• Expressed: HA RAB8B Mito (MAO tag)
• Genbank: NM_016530.3
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCGAAGACGTACGATTATCTCTTCAAGCTCCTGCTGATCGGCGACTCGGGGGTAGGCAAGACCTGCCTCCTGTTCCGCTTCTCAGAGGACGCCTTCAACACCACCTTCATCTCCACCATCGGAATTGATTTTAAAATTAGAACGATAGAACTAGATGGAAAGAAAATTAAGCTTCAGATATGGGACACAGCGGGTCAGGAAAGATTCCGAACAATCACGACAGCGTACTACAGAGGAGCCATGGGCATTATGCTGGTCTATGACATCACAAATGAAAAATCCTTTGACAATATTAAAAATTGGATCAGAAACATTGAAGAGCATGCCTCTTCCGATGTCGAAAGAATGATCCTGGGTAACAAATGTGATATGAATGACAAAAGACAAGTGTCAAAAGAAAGAGGGGAGAAGCTAGCAATTGACTATGGGATTAAATTCTTGGAGACAAGCGCAAAATCCAGTGCAAATGTAGAAGAGGCATTTTTTACACTTGCACGAGATATAATGACAAAACTCAACAGAAAAATGAATGACAGCAATTCAGCAGGAGCAGGTGGACCAGTGAAAATAACAGAAAACCGATCAAAGAAGACCAGTTTCTTTCGTGGTGCTGGCGCCGGTGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMAKTYDYLFKLLLIGDSGVGKTCLLFRFSEDAFNTTFISTIGIDFKIRTIELDGKKIKLQIWDTAGQERFRTITTAYYRGAMGIMLVY
DITNEKSFDNIKNWIRNIEEHASSDVERMILGNKCDMNDKRQVSKERGEKLAIDYGIKFLETSAKSSANVEEAFFTLARDIMTKLNRKMNDSNSAGAGGP
VKITENRSKKTSFFRGAGAGAAAFWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5D HA RAB8B Mito (MAO tag)775.19 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61285
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: HA Rab8B 1-203 (CSLL residues removed at the C-terminus) fused to 3 alanines and a C terminal MAO fragment 489-end (FWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS).
• Low Temp: No