cDNA Clone RAB32

• Plasmid: pCMV5D HA RAB32 Mito (MAO tag)
• Parent Plasmid: pCMV5D HA mcs Mito
• Expressed: HA RAB32 Mito (MAO tag)
• Genbank: NM_006834.3
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCGGGCGGAGGAGCCGGGGACCCCGGCCTGGGGGCGGCTGCCGCCCCAGCGCCCGAGACCCGCGAGCACCTCTTCAAGGTGCTGGTGATCGGCGAGCTTGGCGTGGGCAAGACCAGCATCATCAAGCGCTACGTCCACCAGCTCTTCTCCCAGCACTACCGGGCCACCATCGGGGTGGACTTCGCCCTCAAGGTCCTCAACTGGGACAGCAGGACTCTGGTGCGCCTGCAGCTGTGGGACATCGCGGGGCAGGAGCGATTTGGCAACATGACCCGAGTATACTACAAGGAAGCTGTTGGTGCTTTTGTAGTCTTTGATATATCAAGAAGTTCCACATTTGAGGCAGTCTTAAAATGGAAAAGTGATCTGGATAGTAAAGTTCATCTTCCAAATGGCAGCCCTATCCCTGCTGTCCTCTTGGCTAACAAATGTGACCAGAACAAGGACAGTAGCCAGAGTCCTTCCCAGGTGGACCAATTCTGCAAAGAACATGGCTTTGCCGGATGGTTTGAAACCTCTGCAAAGGATAACATAAACATAGAGGAAGCTGCCCGGTTCCTAGTGGAGAAGATTCTTGTAAACCACCAAAGCTTTCCTAATGAAGAAAACGATGTGGACAAAATTAAGCTAGATCAAGAGACCTTGAGAGCAGAGAACAAATCCCAGGGTGCTGGCGCCGGTGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMAGGGAGDPGLGAAAAPAPETREHLFKVLVIGELGVGKTSIIKRYVHQLFSQHYRATIGVDFALKVLNWDSRTLVRLQLWDIAGQERF
GNMTRVYYKEAVGAFVVFDISRSSTFEAVLKWKSDLDSKVHLPNGSPIPAVLLANKCDQNKDSSQSPSQVDQFCKEHGFAGWFETSAKDNINIEEAARFL
VEKILVNHQSFPNEENDVDKIKLDQETLRAENKSQGAGAGAAAFWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5D HA RAB32 Mito (MAO tag)727.99 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61306
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: HA Rab32 1-223 (CC residues removed in the C-terminus) fused to 3 alanines and a C terminal MAO fragment 489-end (FWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS). Internal Not1 site was killed in insert source.
• Low Temp: No