cDNA Clone CNOT7

• Synonyms: CAF1; CAF-1; Caf1a; hCAF-1
• Plasmid: pCMV5D HA CNOT7 D40A E42A C67E L71E
• Parent Plasmid: pCMV5D HA
• Expressed: HA CNOT7 D40A E42A C67E L71E
• Genbank: NM_013354.7
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCCAGCGGCAACTGTAGATCATAGCCAAAGAATTTGTGAAGTTTGGGCTTGCAACTTGGATGAAGAGATGAAGAAAATTCGTCAAGTTATCCGAAAATATAATTACGTTGCTATGGCCACCGCGTTTCCAGGTGTGGTTGCAAGACCCATTGGAGAATTCAGGAGCAATGCTGACTATCAATACCAACTATTGCGGGAGAATGTAGACGAGTTAAAGATAATTCAGCTAGGACTGACATTTATGAATGAGCAAGGAGAATACCCTCCAGGAACTTCAACTTGGCAGTTTAATTTTAAATTTAATTTGACGGAGGACATGTATGCCCAGGACTCTATAGAGCTACTAACAACATCTGGTATCCAGTTTAAAAAACATGAGGAGGAAGGAATTGAAACCCAGTACTTTGCAGAACTTCTTATGACTTCTGGAGTGGTCCTCTGTGAAGGGGTCAAATGGTTGTCATTTCATAGCGGTTACGACTTTGGCTACTTAATCAAAATCCTAACCAACTCTAACTTGCCTGAAGAAGAACTTGACTTCTTTGAGATCCTTCGATTGTTTTTTCCTGTCATTTATGATGTGAAGTACCTCATGAAGAGCTGCAAAAATCTCAAAGGTGGATTACAGGAGGTGGCAGAACAGTTAGAGCTGGAACGGATAGGACCACAACATCAGGCAGGATCTGATTCATTGCTCACAGGAATGGCCTTTTTCAAAATGAGAGAAATGTTCTTTGAAGATCATATTGATGATGCCAAATATTGTGGTCATTTGTATGGCCTTGGTTCTGGTTCATCCTATGTACAGAATGGCACAGGGAATGCATATGAAGAGGAAGCCAACAAGCAGTCATGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMPAATVDHSQRICEVWACNLDEEMKKIRQVIRKYNYVAMATAFPGVVARPIGEFRSNADYQYQLLRENVDELKIIQLGLTFMNEQGEY
PPGTSTWQFNFKFNLTEDMYAQDSIELLTTSGIQFKKHEEEGIETQYFAELLMTSGVVLCEGVKWLSFHSGYDFGYLIKILTNSNLPEEELDFFEILRLF
FPVIYDVKYLMKSCKNLKGGLQEVAEQLELERIGPQHQAGSDSLLTGMAFFKMREMFFEDHIDDAKYCGHLYGLGSGSSYVQNGTGNAYEEEANKQS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5D HA CNOT7 D40A E42A C67E L71E97.12 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61329
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: D40A/E42A (catalytic core) and C67E/L71E (CNOT6 interaction surface)
• Low Temp: No