cDNA Clone CNOT8

• Synonyms: CAF1; POP2; CALIF; Caf1b; hCAF1
• Plasmid: pCMV5D HA CNOT8 D40A E42A C67E L71E
• Parent Plasmid: pCMV5D HA
• Expressed: HA CNOT8 D40A E42A C67E L71E
• Genbank: NM_004779.6
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCCTGCAGCACTTGTGGAGAATAGCCAGGTTATCTGTGAAGTGTGGGCCAGTAATCTAGAAGAAGAGATGAGGAAGATCCGAGAAATCGTGCTCAGTTACAGTTATATTGCCATGGCCACAGCATTTCCAGGTGTTGTGGTGCGACCAATTGGTGAATTTCGTAGTTCCATAGATTACCAATATCAGCTTCTGCGGGAGAATGTTGACGAGTTAAAAATTATCCAGCTGGGCCTTACATTCACAAATGAGAAGGGAGAGTATCCTTCTGGAATCAATACTTGGCAGTTCAATTTCAAATTTAACCTTACAGAGGACATGTACTCCCAGGATTCCATAGATCTCCTTGCTAACTCAGGACTACAGTTTCAGAAGCATGAAGAGGAAGGGATTGACACACTGCACTTTGCAGAGCTGCTTATGACATCAGGAGTGGTTCTCTGTGACAATGTCAAATGGCTTTCATTTCATAGTGGCTATGATTTTGGCTATATGGTAAAGTTGCTTACAGATTCTCGTTTGCCAGAAGAGGAACATGAATTCTTTCATATTCTGAACCTTTTCTTCCCATCCATTTATGATGTGAAATACCTGATGAAGAGCTGCAAAAATCTTAAGGGAGGTCTTCAGGAAGTTGCTGATCAGTTGGATTTGCAGAGGATTGGAAGGCAGCACCAGGCAGGCTCAGACTCACTGCTGACAGGAATGGCTTTCTTTAGGATGAAAGAGTTGTTTTTTGAGGACAGCATTGATGATGCCAAGTACTGTGGGCGGCTCTATGGCTTAGGCACAGGAGTGGCCCAGAAGCAGAATGAGGATGTGGACTCTGCCCAGGAGAAGATGAGCATCCTGGCGATTATCAACAACATGCAGCAGTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMPAALVENSQVICEVWASNLEEEMRKIREIVLSYSYIAMATAFPGVVVRPIGEFRSSIDYQYQLLRENVDELKIIQLGLTFTNEKGEY
PSGINTWQFNFKFNLTEDMYSQDSIDLLANSGLQFQKHEEEGIDTLHFAELLMTSGVVLCDNVKWLSFHSGYDFGYMVKLLTDSRLPEEEHEFFHILNLF
FPSIYDVKYLMKSCKNLKGGLQEVADQLDLQRIGRQHQAGSDSLLTGMAFFRMKELFFEDSIDDAKYCGRLYGLGTGVAQKQNEDVDSAQEKMSILAIIN
NMQQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5D HA CNOT8 D40A E42A C67E L71E116.38 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61327
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: D40A/E42A (catalytic core) and C67E/L71E (CNOT6 interaction surface)
• Low Temp: No