cDNA Clone TSC22D4 (R71A)

• Synonyms: THG1; THG-1; TILZ2
• Plasmid: pCMV5D HA TSC22D4 R71A
• Parent Plasmid: pCMV5D HA
• Expressed: HA TSC22D4 R71A
• Genbank: NM_030935.5
• Tag: HA
• Species: Human

Sequence of Insert

CTCGAGATGAGCGGGGGCAAGAAGAAGAGTAGTTTCCAAATCACCAGCGTCACCACGGACTATGAGGGCCCTGGGAGCCCAGGGGCTTCGGATCCCCCTACCCCACAGCCCCCAACCGGGCCCCCGCCCCGCCTGCCCAATGGGGAGCCCAGCCCCGATCCGGGGGGCAAGGGCACCCCCCGGAATGGCTCCCCACCACCTGGGGCCCCTTCCTCCGCTTTCCGGGTGGTGAAGCTGCCCCACGGCCTGGGAGAGCCTTATCGCCGCGGTCGCTGGACGTGTGTGGATGTTTATGAGCGAGACCTGGAGCCCCACAGCTTCGGCGGACTCCTGGAGGGAATTCGAGGGGCCTCAGGGGGCGCCGGGGGCAGATCTTTGGATTCCAGGTTGGAGCTGGCCAGCCTCGGCCTGGGCGCCCCCACCCCACCGTCAGGCCTGTCTCAGGGCCCCACCTCCTGGCTCCGTCCACCCCCCACCTCTCCTGGACCTCAGGCCCGCTCCTTCACTGGGGGACTGGGCCAGCTGGTGGTGCCCAGCAAAGCCAAGGCAGAGAAACCCCCACTGTCGGCCTCCTCACCCCAGCAGCGCCCCCCAGAGCCTGAGACCGGTGAGAGTGCGGGCACATCCCGGGCTGCCACGCCCCTGCCCTCTCTGAGGGTGGAAGCGGAGGCTGGGGGCTCAGGGGCCAGGACCCCTCCACTGTCCCGGAGGAAAGCTGTAGACATGCGGCTGCGGATGGAGTTGGGTGCTCCAGAAGAGATGGGGCAGGTGCCCCCACTTGACTCTCGCCCCAGCTCCCCGGCCCTCTACTTCACCCACGATGCCAGCCTGGTTCACAAATCTCCAGACCCCTTCGGAGCAGTAGCAGCTCAGAAGTTCAGCCTGGCCCACTCCATGTTGGCCATCAGTGGTCACCTAGACAGCGACGATGATAGTGGCTCCGGAAGCCTGGTTGGCATTGACAACAAAATCGAGCAAGCCATGGACTTGGTGAAGTCCCACCTCATGTTTGCGGTCCGGGAGGAGGTGGAGGTGCTGAAGGAGCAGATCCGGGAACTGGCGGAGCGGAACGCTGCGCTGGAGCAGGAGAATGGGCTGCTGCGCGCCCTGGCCAGCCCGGAGCAGCTGGCTCAGCTGCCCTCCTCGGGGGTCCCACGGCTTGGGCCCCCTGCGCCCAATGGGCCCTCCGTCTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSFEEFASLEMSGGKKKSSFQITSVTTDYEGPGSPGASDPPTPQPPTGPPPRLPNGEPSPDPGGKGTPRNGSPPPGAPSSAFRVVKLPHG
LGEPYRRGRWTCVDVYERDLEPHSFGGLLEGIRGASGGAGGRSLDSRLELASLGLGAPTPPSGLSQGPTSWLRPPPTSPGPQARSFTGGLGQLVVPSKAK
AEKPPLSASSPQQRPPEPETGESAGTSRAATPLPSLRVEAEAGGSGARTPPLSRRKAVDMRLRMELGAPEEMGQVPPLDSRPSSPALYFTHDASLVHKSP
DPFGAVAAQKFSLAHSMLAISGHLDSDDDSGSGSLVGIDNKIEQAMDLVKSHLMFAVREEVEVLKEQIRELAERNAALEQENGLLRALASPEQLAQLPSS
GVPRLGPPAPNGPSV*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5D HA TSC22D4 R71A60.95 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61342
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Compared with reference sequence there are two silent changes at P265 and L351.
• Low Temp: No