cDNA Clone mouse ALPK1

• Synonyms: 8430410J10Rik; LAK
• Plasmid: pcDNA5D FRT TO FLAG mouse ALPK1 T237M
• Parent Plasmid: pcDNA5D FRT TO FLAG
• Expressed: FLAG mouse ALPK1 T237M
• Genbank: Q9CXB8.2, with a single change E16Q*
• Tag: FLAG
• Species: Mouse

Sequence of Insert

ctcgagatgaataatcaagatgccgtggcttcgatactgcatgagtgcaaggaagtgctggatcgtctcttgttggaaacgccagatgtgtctacagaggacaagagtgaggaccagcggtgcagagcttcactccccagcgagttaaggaccctgatccaggaggcagaggaaatgaagtggcccttcgtgcctgaaaagtggcaatacaaacaagccatgagcccagaggacaaaacaaacctgcaagatgtgattggcgccgggctgcagcagttactggcggccttgcgagcctccatccttgtccaggactgcgctgccgcctccgctattgtcttcttgatggaccggttcctgtatggacttgacgtctccggaaaactcctgcaggtcgccaaaggcttgcacaagctgaagcctgcaacgcccatcgccccgcaggtggtcatccgacaagcccgagtctccgtcaactcagggaaactgttgaaggcagaatacatcctgagcagcctgataagcaacaacggagcaaccggtacctggctgtataggaatgagagtgacaaagtcctggtacaatcggtctgcatacagatcagagggcagattctgcagaagctaggcatgtggtatgaagcggcagaattgatatgggcctccgtcataggatatctgacactccctcagccagataagaagggcatttccatgtcactgggcatactggcagacatctttgtttccatgagcaaaaccgattatgaaaagtttaagaagagcccaaaggttaacttggccctgctcaaggagtttgaccaccatttgttgtcagccgcggaagcctgcaagctggcagctgccttcagtgcctacacacccctctttgtgctcagggctgtgaatatccgtggcacatgtttgttgtcatatagctgttctgctgactgccctccgggaatgaaaagtgtgcatctgtgtgaagccaaagaagcttttgagattggcctcctgaccaagaaagatggcgagctggttagtggcaagcaggagctgcacagcttcatcaaagctgctttcggcctcaccacggtgcactccagactccatggggagacagatgcagttcgcgcagcaaggcagctatgcagtgaagctgtgggcaagctctacaccttcagcacttcccccacaagccaggacagagaaggcctgtctcaggaaatcatgtctctcatcagccaggtgaagggacatctacgagttcaaagcttcccaaatttagatgtttgctcttatgtcccagagagtttcaagtgtggtttagatcgactcatcttgcatggacacgtggatttccaacaaattcttgaaacctattcccagcaccatacttcagtgtgtgaggtatttgaaagcacttgtgggaacagcaaaagcaaccagagagacacaaaatcggaggtctgtattaccactctgaagacagaaacaaacactgcagatactatggttgctactctagagagggtgagttcccaagacagcaggagtacagcttcttccaaaatgtctaagaaagatcaagggaagctccaaagagaaagggggagaagctggacccgttccaaggcattccgagtctccctggatctagatatggagactgagactgagccaccaaatcacagcaatggagggacagatgttttcaacaagtctctgagagacaactctagctcctgttcttggggcagactgtcaggtctcagctcttccacaagctgggaggaagtgaactgcgctgttcaggatgtagtaaggaaagggtctggccaagaaaagcatcctgtggaggcgcaaagctctgaggcagtgtctgaggagcccaagaggaacagaagcagcagagctgtgtttttgtcttcaaagctccgtggtgtctctcttcagacaactggagatgacaacttagagtcttcaccaagtcagttacacaatcacacatccatccttcctttcaatgcaaaagatacttgtttggcctcgggtgcaggactggtagaaaccgctgaaggatctaacaatacttctcttcagtccagtcattcgtgtggttctgattcctggtccttgtctagttcagacaggttcacagatgtgaccacgaatccttcagttcaggaagaagaaccctctggaataatgggtgatgtcccagaatccaagtatgactttaaagactggcacggagaaaagaatgggggaacactcactgaaatatgcacagggcctgaattaacatttgccccctcttcggtcgacccagaaggagaaacagcagaaagcacagacgatggactatcaccctctcaggtagccctaggatgtttagaaggcagccattcaatgtctacgcgtaggactttctttccggatgggtcggttcaaaacgctgactcagcaaagactggctgctcagtcagagaccagactgtcgaccctgatgcctcaacggtggatgaggagggccaaatgcttgacagcacagaggtctgctccattggccaggatggcgctcacagaccacgtgctttgaggtccggtcagagtgcggaggggccaaagtcctttgtaaatggctccagaccttcccctatcttcgatgaggacttcagcaccacagaggaaggagaggagcttgggagcatgctgaagagcagccaaaactccagttcatactcaccatggtggttaaaatcaccagccttttcccgcagttcgtcagatggggaaagctcctggtctctgctgaactcaagcagaagttccttcgcctcgctggcaggacagaccagtcaagagatccttgaggctcgaaccctacagcctgatgaccttgaaaaacttctggcgggcgtgaggcatgattggctgcttcagagactggagaatactggcgttttgaagtccaatcaactccaacaagcccacagtgcccttctgctaaaatattccaaaaagtctgagttgtggacagcgcaggaaactgtggtgtatctgggagactacctgaaagtgaagaagaagggcaagcagagaaatgcattttgggtccactatctccatcaagaggaaaccctggggagatacgttgggaaagaatacaaagagaggaaggggctccggcaccacttcactgatgtggagcggcagatgacagcacagcactacgtgacagagtttaacaagagactctacgagcaaaagattccgactcagatattctacgtcccctctacaatattactgattttggaagacaggactataaagggatgcatcagtgtggagccttacatcctgggggaattcgtcaagttatccaataacaccaaagtagtcaaaaatgagtataaagctacagaatatggcttggcctatggccatttttcttatgagttttctaaccatagagatgtggtggttgatttacaaggttgggtgactggtaatggaaaaggcctgatctatctcacggatcctcagattcactctgtagaccagaaagatgtcactacaaactttggaaagcggggaatattttacttctttaataaccaacatgcaagctgtaatgaaatatgccatcgtctttctctgactagaccttcactagagcaaacaagtaaggtataggcggccgc

Amino Acid Sequence

MDYKDDDDKGSPGEFASLEMNNQDAVASILHECKEVLDRLLLETPDVSTEDKSEDQRCRASLPSELRTLIQEAEEMKWPFVPEKWQYKQAMSPEDKTNLQ
DVIGAGLQQLLAALRASILVQDCAAASAIVFLMDRFLYGLDVSGKLLQVAKGLHKLKPATPIAPQVVIRQARVSVNSGKLLKAEYILSSLISNNGATGTW
LYRNESDKVLVQSVCIQIRGQILQKLGMWYEAAELIWASVIGYLTLPQPDKKGISMSLGILADIFVSMSKTDYEKFKKSPKVNLALLKEFDHHLLSAAEA
CKLAAAFSAYTPLFVLRAVNIRGTCLLSYSCSADCPPGMKSVHLCEAKEAFEIGLLTKKDGELVSGKQELHSFIKAAFGLTTVHSRLHGETDAVRAARQL
CSEAVGKLYTFSTSPTSQDREGLSQEIMSLISQVKGHLRVQSFPNLDVCSYVPESFKCGLDRLILHGHVDFQQILETYSQHHTSVCEVFESTCGNSKSNQ
RDTKSEVCITTLKTETNTADTMVATLERVSSQDSRSTASSKMSKKDQGKLQRERGRSWTRSKAFRVSLDLDMETETEPPNHSNGGTDVFNKSLRDNSSSC
SWGRLSGLSSSTSWEEVNCAVQDVVRKGSGQEKHPVEAQSSEAVSEEPKRNRSSRAVFLSSKLRGVSLQTTGDDNLESSPSQLHNHTSILPFNAKDTCLA
SGAGLVETAEGSNNTSLQSSHSCGSDSWSLSSSDRFTDVTTNPSVQEEEPSGIMGDVPESKYDFKDWHGEKNGGTLTEICTGPELTFAPSSVDPEGETAE
STDDGLSPSQVALGCLEGSHSMSTRRTFFPDGSVQNADSAKTGCSVRDQTVDPDASTVDEEGQMLDSTEVCSIGQDGAHRPRALRSGQSAEGPKSFVNGS
RPSPIFDEDFSTTEEGEELGSMLKSSQNSSSYSPWWLKSPAFSRSSSDGESSWSLLNSSRSSFASLAGQTSQEILEARTLQPDDLEKLLAGVRHDWLLQR
LENTGVLKSNQLQQAHSALLLKYSKKSELWTAQETVVYLGDYLKVKKKGKQRNAFWVHYLHQEETLGRYVGKEYKERKGLRHHFTDVERQMTAQHYVTEF
NKRLYEQKIPTQIFYVPSTILLILEDRTIKGCISVEPYILGEFVKLSNNTKVVKNEYKATEYGLAYGHFSYEFSNHRDVVVDLQGWVTGNGKGLIYLTDP
QIHSVDQKDVTTNFGKRGIFYFFNNQHASCNEICHRLSLTRPSLEQTSKV*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5D FRT TO FLAG mouse ALPK1 T237M1.16 MB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU70206
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *seen in other sequences eg AFE61895.1
• Low Temp: No