cDNA Clone Myosin IIB

• Synonyms: MYH10, NMMHCB, NMMHC-IIB
• Plasmid: pSc-B-MHY10 isoform 1
• Parent Plasmid: pSC-B
• Genbank: Gene ID: 4628 NM_001256012.3 → NP_001242941.1
• Species: Human

Amino Acid Sequence

MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASI
KEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEAS
VLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEM
PPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVAS
SHKGRKDHNIPQESPKPVKHQGELERQLLQANPILESFGNAKTVKNDNSS
RFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERTFHIFYQLLSG
AGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSH
EEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVM
EFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRI
NKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHT
MFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECW
FPKATDKTFVEKLVQEQGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADE
WLMKNMDPLNDNVATLLHQSSDRFVAELWKDEIQNIQRASFYDSVSGLHE
PPVDRIVGLDQVTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLR
NTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRI
VFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIF
FRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVL
QRNCAAYLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQT
KVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQE
LEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKL
QLEKVTAEAKIKKMEEEILLLEDQNSKFIKEKKLMEDRIAECSSQLAEEE
EKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAKRKLDGETTDLQD
QIAELQAQIDELKLQLAKKEEELQGALARGDDETLHKNNALKVVRELQAQ
IAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELR
TKREQEVAELKKALEEETKNHEAQIQDMRQRHATALEELSEQLEQAKRFK
ANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVS
EGDRLRVELAEKASKLQNELDNVSTLLEEAEKKGIKFAKDAASLESQLQD
TQELLQEETRQKLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLAL
QSQLADTKKKVDDDLGTIESLEEAKKKLLKDAEALSQRLEEKALAYDKLE
KTKNRLQQELDDLTVDLDHQRQVASNLEKKQKKFDQLLAEEKSISARYAE
ERDRAEAEAREKETKALSLARALEEALEAKEEFERQNKQLRADMEDLMSS
KDDVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNM
QAMKAQFERDLQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASK
KKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRD
EIFAQSKESEKKLKSLEAEILQLQEELASSERARRHAEQERDELADEITN
SASGKSALLDEKRRLEARIAQLEEELEEEQSNMELLNDRFRKTTLQVDTL
NAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISAL
EAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYK
EQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSR
EVSTLKNRLRRGGPISFSSSRSGRRQLHLEGASLELSDDDTESKTSDVNE
TQPPQSE*

• Vector Type: Other
• Antibiotic: Amp; Kan
• Full Snapgene Map: pSc-B-MHY10 isoform 1 1.15 MB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65951
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform 1 constructed from isoform 2 65919 by:; NW7682/83 Q5 insertion of Q212-Q221 and NW7686/87 Q5 insertion of E632-P652.; Clone contains the following silent changes relative to NCBI reference:; N733 (AAC>AAT); Thr900 (ACG>ACA)
• Low Temp: No