cDNA Clone MYCBP2

• Plasmid: pOPc-Lac-GST-MYCBP2 Lys2458-Asp2553; 6His-SUMO-Fbx045
• Parent Plasmid: pOPc-GST with Lac Operon restored
• Expressed: GST-MYCBP2 Lys2458-Asp2553
• Genbank: NM_015057.4
• Tag: GST
• Species: Human

Sequence of Insert

catatgAAACCAAAGTCTGAACCTCAGCCTAATAAGGTTCGAAAATTTGTGGCCAAGGACAGTGCGGGGCTTCGCATCCGTAGCCACCCTTCCCTTCAGAGTGAGCAGATAGGCATAGTGAAAGTCAATGGAACTATCACTTTTATTGATGAGATCCATAATGATGATGGTGTGTGGCTGAGGCTGAATGATGAGACAATAAAGAAGTATGTCCCTAACATGAATGGTTACACTGAAGCCTGGTGCCTCTCTTTTAATCAACATCTTGGCAAGAGTCTTCTGGTCCCTGTTGACTAAggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPHMKPKSEPQPNKVRKFVAKDSAGLRIRSHPSLQSEQIGIVKVNGTITFIDEIHNDDGVWLRLNDETIKKYVPNMNGYTEAWCLSFNQHLGKSLLVPVD*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pOPc-Lac-GST-MYCBP2 Lys2458-Asp2553; 6His-SUMO-Fbx0451.04 MB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU71133
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: The lac operon was restored to construct 71064 pOPc-GST (into which had already been sucloned a 300bp Nde-Bam flanked MYCBP2 fragment (Lys2458-Asp2553- amplified from clone 29783 with NW7748/49) ; ; The lac operon was restored in a 2 step process:; 1. mutagenic insertion of Lac op downstream of T7 promoter: 18575/18576 into pOPc-GST-MYCBP2 Lys2458-Asp2553 71064; 2. LacI promoter and ORF amplified from pETDuet with RT18577/18578 were then incorporated via NEB Builder into product of step 1 re-amplified with RT18579/18580
• Low Temp: No