cDNA Clone MOK

• Plasmid: pCMV-HA MOK D145A
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-MOK D145A
• Genbank: BAA81688
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGAAGAACTATAAAGCAATTGGCAAAATAGGAGAGGGAACGTT
TTCTGAAGTTATGAAGATGCAAAGCCTGAGAGATGGAAACTACTATGCAT
GTAAACAAATGAAGCAGCGCTTTGAAAGTATTGAGCAAGTCAACAACCTA
CGAGAGATCCAAGCACTGAGGCGCCTGAATCCGCACCCAAACATTCTTAT
GTTGCATGAAGTGGTTTTTGACAGAAAATCTGGTTCTCTTGCACTAATAT
GTGAACTTATGGACATGAATATTTATGAGCTAATACGAGGGAGAAGATAC
CCATTATCAGAAAAAAAAATTATGCACTATATGTACCAGTTATGTAAGTC
CCTGGATCATATTCACAGAAATGGAATATTTCACAGAGATGTAAAACCAG
AAAATATACTAATAAAGCAGGATGTCCTGAAATTAGGGGCCTTTGGCTCC
TGCCGGAGTGTCTATTCCAAGCAGCCGTACACGGAATACATCTCCACCCG
CTGGTACCGGGCCCCGGAGTGTCTCCTCACTGATGGGTTCTACACGTACA
AGATGGACCTGTGGAGCGCCGGCTGTGTGTTCTACGAGATCGCCAGTCTG
CAGCCCCTCTTTCCTGGAGTAAATGAACTGGACCAAATCTCAAAAATCCA
CGATGTCATCGGCACACCCGCTCAGAAGATCCTCACCAAGTTCAAACAGT
CGAGAGCTATGAATTTTGATTTTCCTTTTAAAAAGGGATCAGGAATACCT
CTACTAACAACCAATTTGTCCCCACAATGCCTCTCCCTCCTGCACGCAAT
GGTGGCCTATGATCCCGATGAGAGAATCGCCGCCCACCAGGCCCTGCAGC
ACCCCTACTTCCAAGAACAGAGGAAAACAGAGAAGCGGGCTCTGGGCAGC
CACAGAAAAGCTGGCTTTCCGGAGCACCCTGTGGCACCGGAACCACTCAG
TAACAGCTGCCAGATTTCCAAGGAGGGCAGAAAGCAGAAACAGTCCCTAA
AGCAAGAGGAGGACCGTCCCAAGAGACGAGGACCGGCCTATGTCATGGAA
CTGCCCAAACTAAAGCTTTCGGGAGTGGTCAGACTGTCGTCTTACTCCAG
CCCCACGCTGCAGTCCGTGCTTGGATCTGGAACAAATGGAAGAGTGCCGG
TGCTGAGACCCTTGAAGTGCATCCCTGCGAGCAAGAAGACAGATCCGCAG
AAGGACCTTAAGCCTGCCCCGCAGCAGTGTCGCCTGCCCACCATAGTGCG
GAAAGGCGGAAGATAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMKNYKAIGKIGEGTFSEVMKMQSLRDGNYYACKQMKQR
FESIEQVNNLREIQALRRLNPHPNILMLHEVVFDRKSGSLALICELMDMN
IYELIRGRRYPLSEKKIMHYMYQLCKSLDHIHRNGIFHRDVKPENILIKQ
DVLKLGAFGSCRSVYSKQPYTEYISTRWYRAPECLLTDGFYTYKMDLWSA
GCVFYEIASLQPLFPGVNELDQISKIHDVIGTPAQKILTKFKQSRAMNFD
FPFKKGSGIPLLTTNLSPQCLSLLHAMVAYDPDERIAAHQALQHPYFQEQ
RKTEKRALGSHRKAGFPEHPVAPEPLSNSCQISKEGRKQKQSLKQEEDRP
KRRGPAYVMELPKLKLSGVVRLSSYSSPTLQSVLGSGTNGRVPVLRPLKC
IPASKKTDPQKDLKPAPQQCRLPTIVRKGGR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3924
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression. Kinase dead mutation.; Calc Mol Weight : 49297 Daltons