cDNA Clone NOD1

• Plasmid: pFBHTb HA NOD1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-HA NOD1
• Genbank: AAH40339
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGAAGA
GCAGGGCCACAGTGAGATGGAAATAATCCCATCAGAGTCTCACCCCCACA
TTCAATTACTGAAAAGCAATCGGGAACTTCTGGTCACTCACATCCGCAAT
ACTCAGTGTCTGGTGGACAACTTGCTGAAGAATGACTACTTCTCGGCCGA
AGATGCGGAGATTGTGTGTGCCTGCCCCACCCAGCCTGACAAGGTCCGCA
AAATTCTGGACCTGGTACAGAGCAAGGGCGAGGAGGTGTCCGAGTTCTTC
CTCTACTTGCTCCAGCAACTCGCAGATGCCTACGTGGACCTCAGGCCTTG
GCTGCTGGAGATCGGCTTCTCCCCTTCCCTGCTCACTCAGAGCAAAGTCG
TGGTCAACACTGACCCAGTGAGCAGGTATACCCAGCAGCTGCGACACCAT
CTGGGCCGTGACTCCAAGTTCGTGCTGTGCTATGCCCAGAAGGAGGAGCT
GCTGCTGGAGGAGATCTACATGGACACCATCATGGAGCTGGTTGGCTTCA
GCAATGAGAGCCTGGGCAGCCTGAACAGCCTGGCCTGCCTCCTGGACCAC
ACCACCGGCATCCTCAATGAGCAGGGTGAGACCATCTTCATCCTGGGTGA
TGCTGGGGTGGGCAAGTCCATGCTGCTACAGCGGCTGCAGAGCCTCTGGG
CCACGGGCCGGCTAGACGCAGGGGTCAAATTCTTCTTCCACTTTCGCTGC
CGCATGTTCAGCTGCTTCAAGGAAAGTGACAGGCTGTGTCTGCAGGACCT
GCTCTTCAAGCACTACTGCTACCCAGAGCGGGACCCCGAGGAGGTGTTTG
CCTTCCTGCTGCGCTTCCCCCACGTGGCCCTCTTCACCTTCGATGGCCTG
GACGAGCTGCACTCGGACTTGGACCTGAGCCGCGTGCCTGACAGCTCCTG
CCCCTGGGAGCCTGCCCACCCCCTGGTCTTGCTGGCCAACCTGCTCAGTG
GGAAGCTGCTCAAGGGGGCTAGCAAGCTGCTCACAGCCCGCACAGGCATC
GAGGTCCCGCGCCAGTTCCTGCGGAAGAAGGTGCTTCTCCGGGGCTTCTC
CCCCAGCCACCTGCGCGCCTATGCCAGGAGGATGTTCCCCGAGCGGGCCC
TGCAGGACCGCCTGCTGAGCCAGCTGGAGGCCAACCCCAACCTCTGCAGC
CTGTGCTCTGTGCCCCTCTTCTGCTGGATCATCTTCCGGTGCTTCCAGCA
CTTCCGTGCTGCCTTTGAAGGCTCACCACAGCTGCCCGACTGCACGATGA
CCCTGACAGATGTCTTCCTCCTGGTCACTGAGGTCCATCTGAACAGGATG
CAGCCCAGCAGCCTGGTGCAGCGGAACACACGCAGCCCAGTGGAGACCCT
CCACGCCGGCCGGGACACTCTGTGCTCGCTAGGGCAGGTGGCCCACCGGG
GCATGGAGAAGAGCCTCTTTGTCTTCACCCAGGAGGAGGTGCAGGCCTCC
GGGCTGCAGGAGAGAGACATGCAGCTGGGCTTCCTGCGGGCTTTGCCGGA
GCTGGGCCCCGGGGGTGACCAGCAGTCCTATGAGTTTTTCCACCTCACCC
TCCAGGCCTTCTTTACAGCCTTCTTCCTCGTGCTGGACGACAGGGTGGGT
ACTCAGGAGCTGCTCAGGTTCTTCCAGGAGTGGATGCCCCCTGCGGGGGC
AGCGACCACGTCCTGCTATCCTCCCTTCCTCCCGTTCCAGTGCCTGCAGG
GCAGTGGTCCGGCGCGGGAAGACCTCTTCAAGAACAAGGATCACTTCCAG
TTCACCAACCTCTTCCTGTGCGGGCTGTTGTCCAAAGCCAAACAGAAACT
CCTGCGGCATCTGGTGCCCGCGGCAGCCCTGAGGAGAAAGCGCAAGGCCC
TGTGGGCACACCTGTTTTCCAGCCTGCGGGGCTACCTGAAGAGCCTGCCC
CGCGTTCAGGTCGAAAGCTTCAACCAGGTGCAGGCCATGCCCACGTTCAT
CTGGATGCTGCGCTGCATCTACGAGACACAGAGCCAGAAGGTGGGGCAGC
TGGCGGCCAGGGGCATCTGCGCCAACTACCTCAAGCTGACCTACTGCAAC
GCCTGCTCGGCCGACTGCAGCGCCCTCTCCTTCGTCCTGCATCACTTCCC
CAAGCGGCTGGCCCTAGACCTAGACAACAACAATCTCAACGACTACGGCG
TGCGGGAGCTGCAGCCCTGCTTCAGCCGCCTCACTGTTCTCAGACTCAGC
GTAAACCAGATCACTGACGGTGGGGTAAAGGTGCTAAGCGAAGAGCTGAC
CAAATACAAAATTGTGACCTATTTGGGTTTATACAACAACCAGATCACCG
ATGTCGGAGCCAGGTACGTCACCAAAATCCTGGATGAATGCAAAGGCCTC
ACGCATCTTAAACTGGGAAAAAACAAAATAACAAGTGAAGGAGGGAAGTA
TCTCGCCCTGGCTGTGAAGAACAGCAAATCAATCTCTGAGGTTGGGATGT
GGGGCAATCAAGTTGGGGATGAAGGAGCAAAAGCCTTCGCAGAGGCTCTG
CGGAACCACCCCAGCTTGACCACCCTGAGTCTTGCGTCCAACGGCATCTC
CACAGAAGGAGGAAAGAGCCTTGCGAGGGCCCTGCAGCAGAACACGTCTC
TAGAAATACTGTGGCTGACCCAAAATGAACTCAACGATGAAGTGGCAGAG
AGTTTGGCAGAAATGTTGAAAGTCAACCAGACGTTAAAGCATTTATGGCT
TATCCAGAATCAGATCACAGCTAAGGGGACTGCCCAGCTGGCAGATGCGT
TACAGAGCAACACTGGCATAACAGAGATTTGCCTAAATGGAAACCTGATA
AAACCAGAGGAGGCCAAAGTCTATGAAGATGAGAAGCGGATTATCTGTTT
CTGAGGATCC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSATMYPYDVPDYAMEEQGHSEME
IIPSESHPHIQLLKSNRELLVTHIRNTQCLVDNLLKNDYFSAEDAEIVCA
CPTQPDKVRKILDLVQSKGEEVSEFFLYLLQQLADAYVDLRPWLLEIGFS
PSLLTQSKVVVNTDPVSRYTQQLRHHLGRDSKFVLCYAQKEELLLEEIYM
DTIMELVGFSNESLGSLNSLACLLDHTTGILNEQGETIFILGDAGVGKSM
LLQRLQSLWATGRLDAGVKFFFHFRCRMFSCFKESDRLCLQDLLFKHYCY
PERDPEEVFAFLLRFPHVALFTFDGLDELHSDLDLSRVPDSSCPWEPAHP
LVLLANLLSGKLLKGASKLLTARTGIEVPRQFLRKKVLLRGFSPSHLRAY
ARRMFPERALQDRLLSQLEANPNLCSLCSVPLFCWIIFRCFQHFRAAFEG
SPQLPDCTMTLTDVFLLVTEVHLNRMQPSSLVQRNTRSPVETLHAGRDTL
CSLGQVAHRGMEKSLFVFTQEEVQASGLQERDMQLGFLRALPELGPGGDQ
QSYEFFHLTLQAFFTAFFLVLDDRVGTQELLRFFQEWMPPAGAATTSCYP
PFLPFQCLQGSGPAREDLFKNKDHFQFTNLFLCGLLSKAKQKLLRHLVPA
AALRRKRKALWAHLFSSLRGYLKSLPRVQVESFNQVQAMPTFIWMLRCIY
ETQSQKVGQLAARGICANYLKLTYCNACSADCSALSFVLHHFPKRLALDL
DNNNLNDYGVRELQPCFSRLTVLRLSVNQITDGGVKVLSEELTKYKIVTY
LGLYNNQITDVGARYVTKILDECKGLTHLKLGKNKITSEGGKYLALAVKN
SKSISEVGMWGNQVGDEGAKAFAEALRNHPSLTTLSLASNGISTEGGKSL
ARALQQNTSLEILWLTQNELNDEVAESLAEMLKVNQTLKHLWLIQNQITA
KGTAQLADALQSNTGITEICLNGNLIKPEEAKVYEDEKRIICF*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3907
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His/HA for baculovirus expression. Differs to genbank at H447R. H447R is a standard SNP found in all Europeans ; see ; http://www.ensembl.org/Homo_sapiens/snpview?snp=rs2975634&source=dbSNP; Calc Mol WEight : 112378 Daltons