cDNA Clone NOD1

• Plasmid: pCMV5-HA NOD1
• Parent Plasmid: pCMV5
• Expressed: HA-NOD1
• Genbank: AAH40339
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGAAGA
GCAGGGCCACAGTGAGATGGAAATAATCCCATCAGAGTCTCACCCCCACA
TTCAATTACTGAAAAGCAATCGGGAACTTCTGGTCACTCACATCCGCAAT
ACTCAGTGTCTGGTGGACAACTTGCTGAAGAATGACTACTTCTCGGCCGA
AGATGCGGAGATTGTGTGTGCCTGCCCCACCCAGCCTGACAAGGTCCGCA
AAATTCTGGACCTGGTACAGAGCAAGGGCGAGGAGGTGTCCGAGTTCTTC
CTCTACTTGCTCCAGCAACTCGCAGATGCCTACGTGGACCTCAGGCCTTG
GCTGCTGGAGATCGGCTTCTCCCCTTCCCTGCTCACTCAGAGCAAAGTCG
TGGTCAACACTGACCCAGTGAGCAGGTATACCCAGCAGCTGCGACACCAT
CTGGGCCGTGACTCCAAGTTCGTGCTGTGCTATGCCCAGAAGGAGGAGCT
GCTGCTGGAGGAGATCTACATGGACACCATCATGGAGCTGGTTGGCTTCA
GCAATGAGAGCCTGGGCAGCCTGAACAGCCTGGCCTGCCTCCTGGACCAC
ACCACCGGCATCCTCAATGAGCAGGGTGAGACCATCTTCATCCTGGGTGA
TGCTGGGGTGGGCAAGTCCATGCTGCTACAGCGGCTGCAGAGCCTCTGGG
CCACGGGCCGGCTAGACGCAGGGGTCAAATTCTTCTTCCACTTTCGCTGC
CGCATGTTCAGCTGCTTCAAGGAAAGTGACAGGCTGTGTCTGCAGGACCT
GCTCTTCAAGCACTACTGCTACCCAGAGCGGGACCCCGAGGAGGTGTTTG
CCTTCCTGCTGCGCTTCCCCCACGTGGCCCTCTTCACCTTCGATGGCCTG
GACGAGCTGCACTCGGACTTGGACCTGAGCCGCGTGCCTGACAGCTCCTG
CCCCTGGGAGCCTGCCCACCCCCTGGTCTTGCTGGCCAACCTGCTCAGTG
GGAAGCTGCTCAAGGGGGCTAGCAAGCTGCTCACAGCCCGCACAGGCATC
GAGGTCCCGCGCCAGTTCCTGCGGAAGAAGGTGCTTCTCCGGGGCTTCTC
CCCCAGCCACCTGCGCGCCTATGCCAGGAGGATGTTCCCCGAGCGGGCCC
TGCAGGACCGCCTGCTGAGCCAGCTGGAGGCCAACCCCAACCTCTGCAGC
CTGTGCTCTGTGCCCCTCTTCTGCTGGATCATCTTCCGGTGCTTCCAGCA
CTTCCGTGCTGCCTTTGAAGGCTCACCACAGCTGCCCGACTGCACGATGA
CCCTGACAGATGTCTTCCTCCTGGTCACTGAGGTCCATCTGAACAGGATG
CAGCCCAGCAGCCTGGTGCAGCGGAACACACGCAGCCCAGTGGAGACCCT
CCACGCCGGCCGGGACACTCTGTGCTCGCTAGGGCAGGTGGCCCACCGGG
GCATGGAGAAGAGCCTCTTTGTCTTCACCCAGGAGGAGGTGCAGGCCTCC
GGGCTGCAGGAGAGAGACATGCAGCTGGGCTTCCTGCGGGCTTTGCCGGA
GCTGGGCCCCGGGGGTGACCAGCAGTCCTATGAGTTTTTCCACCTCACCC
TCCAGGCCTTCTTTACAGCCTTCTTCCTCGTGCTGGACGACAGGGTGGGT
ACTCAGGAGCTGCTCAGGTTCTTCCAGGAGTGGATGCCCCCTGCGGGGGC
AGCGACCACGTCCTGCTATCCTCCCTTCCTCCCGTTCCAGTGCCTGCAGG
GCAGTGGTCCGGCGCGGGAAGACCTCTTCAAGAACAAGGATCACTTCCAG
TTCACCAACCTCTTCCTGTGCGGGCTGTTGTCCAAAGCCAAACAGAAACT
CCTGCGGCATCTGGTGCCCGCGGCAGCCCTGAGGAGAAAGCGCAAGGCCC
TGTGGGCACACCTGTTTTCCAGCCTGCGGGGCTACCTGAAGAGCCTGCCC
CGCGTTCAGGTCGAAAGCTTCAACCAGGTGCAGGCCATGCCCACGTTCAT
CTGGATGCTGCGCTGCATCTACGAGACACAGAGCCAGAAGGTGGGGCAGC
TGGCGGCCAGGGGCATCTGCGCCAACTACCTCAAGCTGACCTACTGCAAC
GCCTGCTCGGCCGACTGCAGCGCCCTCTCCTTCGTCCTGCATCACTTCCC
CAAGCGGCTGGCCCTAGACCTAGACAACAACAATCTCAACGACTACGGCG
TGCGGGAGCTGCAGCCCTGCTTCAGCCGCCTCACTGTTCTCAGACTCAGC
GTAAACCAGATCACTGACGGTGGGGTAAAGGTGCTAAGCGAAGAGCTGAC
CAAATACAAAATTGTGACCTATTTGGGTTTATACAACAACCAGATCACCG
ATGTCGGAGCCAGGTACGTCACCAAAATCCTGGATGAATGCAAAGGCCTC
ACGCATCTTAAACTGGGAAAAAACAAAATAACAAGTGAAGGAGGGAAGTA
TCTCGCCCTGGCTGTGAAGAACAGCAAATCAATCTCTGAGGTTGGGATGT
GGGGCAATCAAGTTGGGGATGAAGGAGCAAAAGCCTTCGCAGAGGCTCTG
CGGAACCACCCCAGCTTGACCACCCTGAGTCTTGCGTCCAACGGCATCTC
CACAGAAGGAGGAAAGAGCCTTGCGAGGGCCCTGCAGCAGAACACGTCTC
TAGAAATACTGTGGCTGACCCAAAATGAACTCAACGATGAAGTGGCAGAG
AGTTTGGCAGAAATGTTGAAAGTCAACCAGACGTTAAAGCATTTATGGCT
TATCCAGAATCAGATCACAGCTAAGGGGACTGCCCAGCTGGCAGATGCGT
TACAGAGCAACACTGGCATAACAGAGATTTGCCTAAATGGAAACCTGATA
AAACCAGAGGAGGCCAAAGTCTATGAAGATGAGAAGCGGATTATCTGTTT
CTGAGGATCC

Amino Acid Sequence

MYPYDVPDYAMEEQGHSEMEIIPSESHPHIQLLKSNRELLVTHIRNTQCL
VDNLLKNDYFSAEDAEIVCACPTQPDKVRKILDLVQSKGEEVSEFFLYLL
QQLADAYVDLRPWLLEIGFSPSLLTQSKVVVNTDPVSRYTQQLRHHLGRD
SKFVLCYAQKEELLLEEIYMDTIMELVGFSNESLGSLNSLACLLDHTTGI
LNEQGETIFILGDAGVGKSMLLQRLQSLWATGRLDAGVKFFFHFRCRMFS
CFKESDRLCLQDLLFKHYCYPERDPEEVFAFLLRFPHVALFTFDGLDELH
SDLDLSRVPDSSCPWEPAHPLVLLANLLSGKLLKGASKLLTARTGIEVPR
QFLRKKVLLRGFSPSHLRAYARRMFPERALQDRLLSQLEANPNLCSLCSV
PLFCWIIFRCFQHFRAAFEGSPQLPDCTMTLTDVFLLVTEVHLNRMQPSS
LVQRNTRSPVETLHAGRDTLCSLGQVAHRGMEKSLFVFTQEEVQASGLQE
RDMQLGFLRALPELGPGGDQQSYEFFHLTLQAFFTAFFLVLDDRVGTQEL
LRFFQEWMPPAGAATTSCYPPFLPFQCLQGSGPAREDLFKNKDHFQFTNL
FLCGLLSKAKQKLLRHLVPAAALRRKRKALWAHLFSSLRGYLKSLPRVQV
ESFNQVQAMPTFIWMLRCIYETQSQKVGQLAARGICANYLKLTYCNACSA
DCSALSFVLHHFPKRLALDLDNNNLNDYGVRELQPCFSRLTVLRLSVNQI
TDGGVKVLSEELTKYKIVTYLGLYNNQITDVGARYVTKILDECKGLTHLK
LGKNKITSEGGKYLALAVKNSKSISEVGMWGNQVGDEGAKAFAEALRNHP
SLTTLSLASNGISTEGGKSLARALQQNTSLEILWLTQNELNDEVAESLAE
MLKVNQTLKHLWLIQNQITAKGTAQLADALQSNTGITEICLNGNLIKPEE
AKVYEDEKRIICF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3906
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression. Differs to genbank at H447R. H447R is a standard SNP found in all Europeans ; see ; http://www.ensembl.org/Homo_sapiens/snpview?snp=rs2975634&source=dbSNP; Calc Mol WEight : 108836 Daltons