cDNA Clone NLK

• Plasmid: pCMV-HA NLK D270A
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-NLK D270A
• Genbank: NP_057315
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCGGCTTACAATGGCGGTACATCTGCAGCAGCAACAGGTCA
CCACCACCACCATCACCACCACCTTCCACACCTCCCTCCTCCTCACCTGC
ACCACCACCACCACCCTCAACACCATCTTCATCCGGGGTCGGCTGCCGCT
GTACACCCTGTACAGCAGCACACCTCTTCGGCAGCTGCGGCAGCCGCAGC
AGCGGCTGCAGCTGCAGCCATGTTAAACCCTGGGCAACAACAGCCATATT
TCCCATCACCGGCACCGGGGCAGGCTCCTGGACCAGCTGCAGCAGCCCCA
GCTCAGGTACAGGCTGCCGCAGCTGCTACAGTTAAGGCGCACCATCATCA
GCACTCGCATCATCCACAGCAGCAGCTGGATATTGAGCCGGATAGACCTA
TTGGATATGGAGCCTTTGGTGTTGTCTGGTCAGTAACAGATCCAAGAGAT
GGAAAGAGAGTAGCGCTCAAAAAGATGCCCAACGTCTTCCAGAATCTGGT
CTCTTGCAAAAGGGTCTTCCGGGAATTGAAGATGTTGTGTTTTTTTAAGC
ATGATAATGTACTCTCTGCCCTTGACATACTCCAACCTCCACACATTGAC
TATTTTGAAGAAATATATGTTGTCACAGAATTGATGCAGAGTGACCTACA
TAAAATTATCGTCTCTCCTCAACCACTCAGCTCAGATCATGTCAAAGTTT
TTCTTTATCAGATTTTGCGAGGTTTGAAATATCTCCATTCAGCTGGCATT
TTACATCGAGACATTAAGCCAGGGAATCTCCTTGTGAACAGCAACTGTGT
TCTAAAGATTTGTGCTTTTGGATTGGCCAGAGTGGAAGAATTAGATGAAT
CCCGTCATATGACTCAGGAAGTTGTTACTCAGTATTATCGGGCTCCAGAA
ATCCTGATGGGCAGCCGTCATTACAGCAATGCTATTGACATCTGGTCTGT
GGGATGTATCTTTGCAGAACTACTAGGACGAAGAATATTGTTTCAGGCAC
AGAGTCCCATTCAGCAGTTGGATTTGATCACGGATCTGTTGGGCACACCA
TCACTGGAAGCAATGAGGACAGCTTGTGAAGGCGCTAAGGCACATATACT
CAGGGGTCCTCATAAACAGCCATCTCTTCCTGTACTCTATACCCTGTCTA
GCCAGGCTACACATGAAGCTGTTCATCTCCTTTGCAGGATGTTGGTCTTT
GATCCATCCAAAAGAATATCCGCTAAGGATGCCTTAGCCCACCCCTACCT
AGATGAAGGGCGACTACGATATCACACATGTATGTGTAAATGTTGCTTTT
CCACCTCCACTGGAAGAGTTTATACCAGTGACTTTGAGCCTGTCACCAAT
CCCAAATTTGATGACACTTTCGAGAAGAACCTCAGTTCTGTCCGACAGGT
TAAAGAAATTATTCATCAGTTCATTTTGGAACAGCAGAAAGGAAACAGAG
TGCCTCTCTGCATCAACCCTCAGTCTGCTGCTTTTAAGAGCTTTATTAGT
TCCACTGTTGCTCAGCCATCTGAGATGCCCCCATCTCCTCTGGTGTGGGA
GTGAGGATCC

Amino Acid Sequence

MYPYDVPDYAGSMAAYNGGTSAAATGHHHHHHHHLPHLPPPHLHHHHHPQ
HHLHPGSAAAVHPVQQHTSSAAAAAAAAAAAAAMLNPGQQQPYFPSPAPG
QAPGPAAAAPAQVQAAAAATVKAHHHQHSHHPQQQLDIEPDRPIGYGAFG
VVWSVTDPRDGKRVALKKMPNVFQNLVSCKRVFRELKMLCFFKHDNVLSA
LDILQPPHIDYFEEIYVVTELMQSDLHKIIVSPQPLSSDHVKVFLYQILR
GLKYLHSAGILHRDIKPGNLLVNSNCVLKICAFGLARVEELDESRHMTQE
VVTQYYRAPEILMGSRHYSNAIDIWSVGCIFAELLGRRILFQAQSPIQQL
DLITDLLGTPSLEAMRTACEGAKAHILRGPHKQPSLPVLYTLSSQATHEA
VHLLCRMLVFDPSKRISAKDALAHPYLDEGRLRYHTCMCKCCFSTSTGRV
YTSDFEPVTNPKFDDTFEKNLSSVRQVKEIIHQFILEQQKGNRVPLCINP
QSAAFKSFISSTVAQPSEMPPSPLVWE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3900
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression. Kinase dead mutation.; Calc Mol Weight : 58356 Daltons