cDNA Clone NOD1

• Plasmid: pGEX6P-1-HA NOD1
• Parent Plasmid: pGEX6P1
• Expressed: GST-HA NOD1
• Genbank: AAH40339
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGAAGA
GCAGGGCCACAGTGAGATGGAAATAATCCCATCAGAGTCTCACCCCCACA
TTCAATTACTGAAAAGCAATCGGGAACTTCTGGTCACTCACATCCGCAAT
ACTCAGTGTCTGGTGGACAACTTGCTGAAGAATGACTACTTCTCGGCCGA
AGATGCGGAGATTGTGTGTGCCTGCCCCACCCAGCCTGACAAGGTCCGCA
AAATTCTGGACCTGGTACAGAGCAAGGGCGAGGAGGTGTCCGAGTTCTTC
CTCTACTTGCTCCAGCAACTCGCAGATGCCTACGTGGACCTCAGGCCTTG
GCTGCTGGAGATCGGCTTCTCCCCTTCCCTGCTCACTCAGAGCAAAGTCG
TGGTCAACACTGACCCAGTGAGCAGGTATACCCAGCAGCTGCGACACCAT
CTGGGCCGTGACTCCAAGTTCGTGCTGTGCTATGCCCAGAAGGAGGAGCT
GCTGCTGGAGGAGATCTACATGGACACCATCATGGAGCTGGTTGGCTTCA
GCAATGAGAGCCTGGGCAGCCTGAACAGCCTGGCCTGCCTCCTGGACCAC
ACCACCGGCATCCTCAATGAGCAGGGTGAGACCATCTTCATCCTGGGTGA
TGCTGGGGTGGGCAAGTCCATGCTGCTACAGCGGCTGCAGAGCCTCTGGG
CCACGGGCCGGCTAGACGCAGGGGTCAAATTCTTCTTCCACTTTCGCTGC
CGCATGTTCAGCTGCTTCAAGGAAAGTGACAGGCTGTGTCTGCAGGACCT
GCTCTTCAAGCACTACTGCTACCCAGAGCGGGACCCCGAGGAGGTGTTTG
CCTTCCTGCTGCGCTTCCCCCACGTGGCCCTCTTCACCTTCGATGGCCTG
GACGAGCTGCACTCGGACTTGGACCTGAGCCGCGTGCCTGACAGCTCCTG
CCCCTGGGAGCCTGCCCACCCCCTGGTCTTGCTGGCCAACCTGCTCAGTG
GGAAGCTGCTCAAGGGGGCTAGCAAGCTGCTCACAGCCCGCACAGGCATC
GAGGTCCCGCGCCAGTTCCTGCGGAAGAAGGTGCTTCTCCGGGGCTTCTC
CCCCAGCCACCTGCGCGCCTATGCCAGGAGGATGTTCCCCGAGCGGGCCC
TGCAGGACCGCCTGCTGAGCCAGCTGGAGGCCAACCCCAACCTCTGCAGC
CTGTGCTCTGTGCCCCTCTTCTGCTGGATCATCTTCCGGTGCTTCCAGCA
CTTCCGTGCTGCCTTTGAAGGCTCACCACAGCTGCCCGACTGCACGATGA
CCCTGACAGATGTCTTCCTCCTGGTCACTGAGGTCCATCTGAACAGGATG
CAGCCCAGCAGCCTGGTGCAGCGGAACACACGCAGCCCAGTGGAGACCCT
CCACGCCGGCCGGGACACTCTGTGCTCGCTAGGGCAGGTGGCCCACCGGG
GCATGGAGAAGAGCCTCTTTGTCTTCACCCAGGAGGAGGTGCAGGCCTCC
GGGCTGCAGGAGAGAGACATGCAGCTGGGCTTCCTGCGGGCTTTGCCGGA
GCTGGGCCCCGGGGGTGACCAGCAGTCCTATGAGTTTTTCCACCTCACCC
TCCAGGCCTTCTTTACAGCCTTCTTCCTCGTGCTGGACGACAGGGTGGGT
ACTCAGGAGCTGCTCAGGTTCTTCCAGGAGTGGATGCCCCCTGCGGGGGC
AGCGACCACGTCCTGCTATCCTCCCTTCCTCCCGTTCCAGTGCCTGCAGG
GCAGTGGTCCGGCGCGGGAAGACCTCTTCAAGAACAAGGATCACTTCCAG
TTCACCAACCTCTTCCTGTGCGGGCTGTTGTCCAAAGCCAAACAGAAACT
CCTGCGGCATCTGGTGCCCGCGGCAGCCCTGAGGAGAAAGCGCAAGGCCC
TGTGGGCACACCTGTTTTCCAGCCTGCGGGGCTACCTGAAGAGCCTGCCC
CGCGTTCAGGTCGAAAGCTTCAACCAGGTGCAGGCCATGCCCACGTTCAT
CTGGATGCTGCGCTGCATCTACGAGACACAGAGCCAGAAGGTGGGGCAGC
TGGCGGCCAGGGGCATCTGCGCCAACTACCTCAAGCTGACCTACTGCAAC
GCCTGCTCGGCCGACTGCAGCGCCCTCTCCTTCGTCCTGCATCACTTCCC
CAAGCGGCTGGCCCTAGACCTAGACAACAACAATCTCAACGACTACGGCG
TGCGGGAGCTGCAGCCCTGCTTCAGCCGCCTCACTGTTCTCAGACTCAGC
GTAAACCAGATCACTGACGGTGGGGTAAAGGTGCTAAGCGAAGAGCTGAC
CAAATACAAAATTGTGACCTATTTGGGTTTATACAACAACCAGATCACCG
ATGTCGGAGCCAGGTACGTCACCAAAATCCTGGATGAATGCAAAGGCCTC
ACGCATCTTAAACTGGGAAAAAACAAAATAACAAGTGAAGGAGGGAAGTA
TCTCGCCCTGGCTGTGAAGAACAGCAAATCAATCTCTGAGGTTGGGATGT
GGGGCAATCAAGTTGGGGATGAAGGAGCAAAAGCCTTCGCAGAGGCTCTG
CGGAACCACCCCAGCTTGACCACCCTGAGTCTTGCGTCCAACGGCATCTC
CACAGAAGGAGGAAAGAGCCTTGCGAGGGCCCTGCAGCAGAACACGTCTC
TAGAAATACTGTGGCTGACCCAAAATGAACTCAACGATGAAGTGGCAGAG
AGTTTGGCAGAAATGTTGAAAGTCAACCAGACGTTAAAGCATTTATGGCT
TATCCAGAATCAGATCACAGCTAAGGGGACTGCCCAGCTGGCAGATGCGT
TACAGAGCAACACTGGCATAACAGAGATTTGCCTAAATGGAAACCTGATA
AAACCAGAGGAGGCCAAAGTCTATGAAGATGAGAAGCGGATTATCTGTTT
CTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMYPYDVPDYAMEEQGHS
EMEIIPSESHPHIQLLKSNRELLVTHIRNTQCLVDNLLKNDYFSAEDAEI
VCACPTQPDKVRKILDLVQSKGEEVSEFFLYLLQQLADAYVDLRPWLLEI
GFSPSLLTQSKVVVNTDPVSRYTQQLRHHLGRDSKFVLCYAQKEELLLEE
IYMDTIMELVGFSNESLGSLNSLACLLDHTTGILNEQGETIFILGDAGVG
KSMLLQRLQSLWATGRLDAGVKFFFHFRCRMFSCFKESDRLCLQDLLFKH
YCYPERDPEEVFAFLLRFPHVALFTFDGLDELHSDLDLSRVPDSSCPWEP
AHPLVLLANLLSGKLLKGASKLLTARTGIEVPRQFLRKKVLLRGFSPSHL
RAYARRMFPERALQDRLLSQLEANPNLCSLCSVPLFCWIIFRCFQHFRAA
FEGSPQLPDCTMTLTDVFLLVTEVHLNRMQPSSLVQRNTRSPVETLHAGR
DTLCSLGQVAHRGMEKSLFVFTQEEVQASGLQERDMQLGFLRALPELGPG
GDQQSYEFFHLTLQAFFTAFFLVLDDRVGTQELLRFFQEWMPPAGAATTS
CYPPFLPFQCLQGSGPAREDLFKNKDHFQFTNLFLCGLLSKAKQKLLRHL
VPAAALRRKRKALWAHLFSSLRGYLKSLPRVQVESFNQVQAMPTFIWMLR
CIYETQSQKVGQLAARGICANYLKLTYCNACSADCSALSFVLHHFPKRLA
LDLDNNNLNDYGVRELQPCFSRLTVLRLSVNQITDGGVKVLSEELTKYKI
VTYLGLYNNQITDVGARYVTKILDECKGLTHLKLGKNKITSEGGKYLALA
VKNSKSISEVGMWGNQVGDEGAKAFAEALRNHPSLTTLSLASNGISTEGG
KSLARALQQNTSLEILWLTQNELNDEVAESLAEMLKVNQTLKHLWLIQNQ
ITAKGTAQLADALQSNTGITEICLNGNLIKPEEAKVYEDEKRIICF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3891
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/HA for bacterial expression. Differs to genbank at H447R. H447R is a standard SNP found in all Europeans ; see ; http://www.ensembl.org/Homo_sapiens/snpview?snp=rs2975634&source=dbSNP; Calc Mol Weight : 135815 Daltons