cDNA Clone PLC delta (mouse)

• Plasmid: pGEX-PLC delta
• Parent Plasmid: pGEX4T1
• Expressed: GST-PLC delta
• Genbank: BC025798
• Tag: GST

Sequence of Insert

GGATCCCCAGGAATTCGCCCTTCGTCCCCTCCTCCGCGCGCCCCGACCGG
CATGGACTCCGGTCGGGACTTCCTGACCTTGCACGGGCTGCAGGATGACC
CGGACCTTCAGGCCCTCCTGAAGGGCAGCCAGCTTCTGAAGGTGAAATCC
AGCTCGTGGCGTAGAGAACGCTTCTACAAGCTACAGGAGGACTGCAAGAC
CATCTGGCAGGAATCTCGCAAGGTCATGAGGTCCCCGGAGTCGCAGCTGT
TCTCCATCGAGGACATTCAGGAGGTGCGGATGGGACACCGCACAGAAGGC
CTGGAGAAATTTGCCAGAGACATACCCGAGGACCGATGCTTCTCCATCGT
CTTCAAGGACCAGCGCAATACCCTAGACCTCATCGCCCCGTCTCCAGCTG
ACGTCCAGCATTGGGTGCAGGGCCTGCGCAAGATCATCGACCGCTCGGGC
TCCATGGACCAGCGGCAGAAGCTGCAGCACTGGATTCACTCTTGCTTGCG
AAAGGCTGATAAAAACAAGGATAACAAGATGAACTTCAAGGAGGTGAAGG
ACTTCCTGAAGGAGCTCAACGTACAGGTGGATGACAGCAAGGGCGAGATT
CCCGGGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPGIRPSSPPPRAPTGMDSGRDFLT
LHGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVM
RSPESQLFSIEDIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQRNTLD
LIAPSPADVQHWVQGLRKIIDRSGSMDQRQKLQHWIHSCLRKADKNKDNK
MNFKEVKDFLKELNVQVDDSKGEIPGSTRAAAS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3871
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct does not have a stop so will express; KGEIPGSTRAAAS; from pGEX MCS.; Calc Mol WEight : 50101 Daltons