cDNA Clone NLK

• Plasmid: pGEX6P-1-NLK D270A
• Parent Plasmid: pGEX6P1
• Expressed: GST-NLK D270A
• Genbank: NP_057315
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGCTTACAATGGCGGTACATCTGCAGCAGCAACAGGTCA
CCACCACCACCATCACCACCACCTTCCACACCTCCCTCCTCCTCACCTGC
ACCACCACCACCACCCTCAACACCATCTTCATCCGGGGTCGGCTGCCGCT
GTACACCCTGTACAGCAGCACACCTCTTCGGCAGCTGCGGCAGCCGCAGC
AGCGGCTGCAGCTGCAGCCATGTTAAACCCTGGGCAACAACAGCCATATT
TCCCATCACCGGCACCGGGGCAGGCTCCTGGACCAGCTGCAGCAGCCCCA
GCTCAGGTACAGGCTGCCGCAGCTGCTACAGTTAAGGCGCACCATCATCA
GCACTCGCATCATCCACAGCAGCAGCTGGATATTGAGCCGGATAGACCTA
TTGGATATGGAGCCTTTGGTGTTGTCTGGTCAGTAACAGATCCAAGAGAT
GGAAAGAGAGTAGCGCTCAAAAAGATGCCCAACGTCTTCCAGAATCTGGT
CTCTTGCAAAAGGGTCTTCCGGGAATTGAAGATGTTGTGTTTTTTTAAGC
ATGATAATGTACTCTCTGCCCTTGACATACTCCAACCTCCACACATTGAC
TATTTTGAAGAAATATATGTTGTCACAGAATTGATGCAGAGTGACCTACA
TAAAATTATCGTCTCTCCTCAACCACTCAGCTCAGATCATGTCAAAGTTT
TTCTTTATCAGATTTTGCGAGGTTTGAAATATCTCCATTCAGCTGGCATT
TTACATCGAGACATTAAGCCAGGGAATCTCCTTGTGAACAGCAACTGTGT
TCTAAAGATTTGTGCTTTTGGATTGGCCAGAGTGGAAGAATTAGATGAAT
CCCGTCATATGACTCAGGAAGTTGTTACTCAGTATTATCGGGCTCCAGAA
ATCCTGATGGGCAGCCGTCATTACAGCAATGCTATTGACATCTGGTCTGT
GGGATGTATCTTTGCAGAACTACTAGGACGAAGAATATTGTTTCAGGCAC
AGAGTCCCATTCAGCAGTTGGATTTGATCACGGATCTGTTGGGCACACCA
TCACTGGAAGCAATGAGGACAGCTTGTGAAGGCGCTAAGGCACATATACT
CAGGGGTCCTCATAAACAGCCATCTCTTCCTGTACTCTATACCCTGTCTA
GCCAGGCTACACATGAAGCTGTTCATCTCCTTTGCAGGATGTTGGTCTTT
GATCCATCCAAAAGAATATCCGCTAAGGATGCCTTAGCCCACCCCTACCT
AGATGAAGGGCGACTACGATATCACACATGTATGTGTAAATGTTGCTTTT
CCACCTCCACTGGAAGAGTTTATACCAGTGACTTTGAGCCTGTCACCAAT
CCCAAATTTGATGACACTTTCGAGAAGAACCTCAGTTCTGTCCGACAGGT
TAAAGAAATTATTCATCAGTTCATTTTGGAACAGCAGAAAGGAAACAGAG
TGCCTCTCTGCATCAACCCTCAGTCTGCTGCTTTTAAGAGCTTTATTAGT
TCCACTGTTGCTCAGCCATCTGAGATGCCCCCATCTCCTCTGGTGTGGGA
GTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAAYNGGTSAAATGHHHHH
HHHLPHLPPPHLHHHHHPQHHLHPGSAAAVHPVQQHTSSAAAAAAAAAAA
AAMLNPGQQQPYFPSPAPGQAPGPAAAAPAQVQAAAAATVKAHHHQHSHH
PQQQLDIEPDRPIGYGAFGVVWSVTDPRDGKRVALKKMPNVFQNLVSCKR
VFRELKMLCFFKHDNVLSALDILQPPHIDYFEEIYVVTELMQSDLHKIIV
SPQPLSSDHVKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSNCVLKIC
AFGLARVEELDESRHMTQEVVTQYYRAPEILMGSRHYSNAIDIWSVGCIF
AELLGRRILFQAQSPIQQLDLITDLLGTPSLEAMRTACEGAKAHILRGPH
KQPSLPVLYTLSSQATHEAVHLLCRMLVFDPSKRISAKDALAHPYLDEGR
LRYHTCMCKCCFSTSTGRVYTSDFEPVTNPKFDDTFEKNLSSVRQVKEII
HQFILEQQKGNRVPLCINPQSAAFKSFISSTVAQPSEMPPSPLVWE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3866
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression. Kinase dead mutation.; Calc Mol Weight : 83808 Daltons