cDNA Clone NLK

• Plasmid: pFBHTb NLK
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-NLK
• Genbank: NP_057315
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGGCTTACAATGGCGGTACATCTGCAGCAGCAACAGGTCA
CCACCACCACCATCACCACCACCTTCCACACCTCCCTCCTCCTCACCTGC
ACCACCACCACCACCCTCAACACCATCTTCATCCGGGGTCGGCTGCCGCT
GTACACCCTGTACAGCAGCACACCTCTTCGGCAGCTGCGGCAGCCGCAGC
AGCGGCTGCAGCTGCAGCCATGTTAAACCCTGGGCAACAACAGCCATATT
TCCCATCACCGGCACCGGGGCAGGCTCCTGGACCAGCTGCAGCAGCCCCA
GCTCAGGTACAGGCTGCCGCAGCTGCTACAGTTAAGGCGCACCATCATCA
GCACTCGCATCATCCACAGCAGCAGCTGGATATTGAGCCGGATAGACCTA
TTGGATATGGAGCCTTTGGTGTTGTCTGGTCAGTAACAGATCCAAGAGAT
GGAAAGAGAGTAGCGCTCAAAAAGATGCCCAACGTCTTCCAGAATCTGGT
CTCTTGCAAAAGGGTCTTCCGGGAATTGAAGATGTTGTGTTTTTTTAAGC
ATGATAATGTACTCTCTGCCCTTGACATACTCCAACCTCCACACATTGAC
TATTTTGAAGAAATATATGTTGTCACAGAATTGATGCAGAGTGACCTACA
TAAAATTATCGTCTCTCCTCAACCACTCAGCTCAGATCATGTCAAAGTTT
TTCTTTATCAGATTTTGCGAGGTTTGAAATATCTCCATTCAGCTGGCATT
TTACATCGAGACATTAAGCCAGGGAATCTCCTTGTGAACAGCAACTGTGT
TCTAAAGATTTGTGATTTTGGATTGGCCAGAGTGGAAGAATTAGATGAAT
CCCGTCATATGACTCAGGAAGTTGTTACTCAGTATTATCGGGCTCCAGAA
ATCCTGATGGGCAGCCGTCATTACAGCAATGCTATTGACATCTGGTCTGT
GGGATGTATCTTTGCAGAACTACTAGGACGAAGAATATTGTTTCAGGCAC
AGAGTCCCATTCAGCAGTTGGATTTGATCACGGATCTGTTGGGCACACCA
TCACTGGAAGCAATGAGGACAGCTTGTGAAGGCGCTAAGGCACATATACT
CAGGGGTCCTCATAAACAGCCATCTCTTCCTGTACTCTATACCCTGTCTA
GCCAGGCTACACATGAAGCTGTTCATCTCCTTTGCAGGATGTTGGTCTTT
GATCCATCCAAAAGAATATCCGCTAAGGATGCCTTAGCCCACCCCTACCT
AGATGAAGGGCGACTACGATATCACACATGTATGTGTAAATGTTGCTTTT
CCACCTCCACTGGAAGAGTTTATACCAGTGACTTTGAGCCTGTCACCAAT
CCCAAATTTGATGACACTTTCGAGAAGAACCTCAGTTCTGTCCGACAGGT
TAAAGAAATTATTCATCAGTTCATTTTGGAACAGCAGAAAGGAAACAGAG
TGCCTCTCTGCATCAACCCTCAGTCTGCTGCTTTTAAGAGCTTTATTAGT
TCCACTGTTGCTCAGCCATCTGAGATGCCCCCATCTCCTCTGGTGTGGGA
GTGAGGATCC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMAAYNGGTSAAATGHHHHHHHH
LPHLPPPHLHHHHHPQHHLHPGSAAAVHPVQQHTSSAAAAAAAAAAAAAM
LNPGQQQPYFPSPAPGQAPGPAAAAPAQVQAAAAATVKAHHHQHSHHPQQ
QLDIEPDRPIGYGAFGVVWSVTDPRDGKRVALKKMPNVFQNLVSCKRVFR
ELKMLCFFKHDNVLSALDILQPPHIDYFEEIYVVTELMQSDLHKIIVSPQ
PLSSDHVKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSNCVLKICDFG
LARVEELDESRHMTQEVVTQYYRAPEILMGSRHYSNAIDIWSVGCIFAEL
LGRRILFQAQSPIQQLDLITDLLGTPSLEAMRTACEGAKAHILRGPHKQP
SLPVLYTLSSQATHEAVHLLCRMLVFDPSKRISAKDALAHPYLDEGRLRY
HTCMCKCCFSTSTGRVYTSDFEPVTNPKFDDTFEKNLSSVRQVKEIIHQF
ILEQQKGNRVPLCINPQSAAFKSFISSTVAQPSEMPPSPLVWE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3849
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression. ; Calc Mol Weight : 60411 Daltons