cDNA Clone NLK

• Plasmid: pEBG6P-NLK
• Parent Plasmid: pEBG6P
• Expressed: GST-NLK
• Genbank: NP_057315
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGCTTACAATGGCGGTACATCTGCAGCAGCAACAGGTCA
CCACCACCACCATCACCACCACCTTCCACACCTCCCTCCTCCTCACCTGC
ACCACCACCACCACCCTCAACACCATCTTCATCCGGGGTCGGCTGCCGCT
GTACACCCTGTACAGCAGCACACCTCTTCGGCAGCTGCGGCAGCCGCAGC
AGCGGCTGCAGCTGCAGCCATGTTAAACCCTGGGCAACAACAGCCATATT
TCCCATCACCGGCACCGGGGCAGGCTCCTGGACCAGCTGCAGCAGCCCCA
GCTCAGGTACAGGCTGCCGCAGCTGCTACAGTTAAGGCGCACCATCATCA
GCACTCGCATCATCCACAGCAGCAGCTGGATATTGAGCCGGATAGACCTA
TTGGATATGGAGCCTTTGGTGTTGTCTGGTCAGTAACAGATCCAAGAGAT
GGAAAGAGAGTAGCGCTCAAAAAGATGCCCAACGTCTTCCAGAATCTGGT
CTCTTGCAAAAGGGTCTTCCGGGAATTGAAGATGTTGTGTTTTTTTAAGC
ATGATAATGTACTCTCTGCCCTTGACATACTCCAACCTCCACACATTGAC
TATTTTGAAGAAATATATGTTGTCACAGAATTGATGCAGAGTGACCTACA
TAAAATTATCGTCTCTCCTCAACCACTCAGCTCAGATCATGTCAAAGTTT
TTCTTTATCAGATTTTGCGAGGTTTGAAATATCTCCATTCAGCTGGCATT
TTACATCGAGACATTAAGCCAGGGAATCTCCTTGTGAACAGCAACTGTGT
TCTAAAGATTTGTGATTTTGGATTGGCCAGAGTGGAAGAATTAGATGAAT
CCCGTCATATGACTCAGGAAGTTGTTACTCAGTATTATCGGGCTCCAGAA
ATCCTGATGGGCAGCCGTCATTACAGCAATGCTATTGACATCTGGTCTGT
GGGATGTATCTTTGCAGAACTACTAGGACGAAGAATATTGTTTCAGGCAC
AGAGTCCCATTCAGCAGTTGGATTTGATCACGGATCTGTTGGGCACACCA
TCACTGGAAGCAATGAGGACAGCTTGTGAAGGCGCTAAGGCACATATACT
CAGGGGTCCTCATAAACAGCCATCTCTTCCTGTACTCTATACCCTGTCTA
GCCAGGCTACACATGAAGCTGTTCATCTCCTTTGCAGGATGTTGGTCTTT
GATCCATCCAAAAGAATATCCGCTAAGGATGCCTTAGCCCACCCCTACCT
AGATGAAGGGCGACTACGATATCACACATGTATGTGTAAATGTTGCTTTT
CCACCTCCACTGGAAGAGTTTATACCAGTGACTTTGAGCCTGTCACCAAT
CCCAAATTTGATGACACTTTCGAGAAGAACCTCAGTTCTGTCCGACAGGT
TAAAGAAATTATTCATCAGTTCATTTTGGAACAGCAGAAAGGAAACAGAG
TGCCTCTCTGCATCAACCCTCAGTCTGCTGCTTTTAAGAGCTTTATTAGT
TCCACTGTTGCTCAGCCATCTGAGATGCCCCCATCTCCTCTGGTGTGGGA
GTGAGGATCC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAAYNGGTSAAAT
GHHHHHHHHLPHLPPPHLHHHHHPQHHLHPGSAAAVHPVQQHTSSAAAAA
AAAAAAAAMLNPGQQQPYFPSPAPGQAPGPAAAAPAQVQAAAAATVKAHH
HQHSHHPQQQLDIEPDRPIGYGAFGVVWSVTDPRDGKRVALKKMPNVFQN
LVSCKRVFRELKMLCFFKHDNVLSALDILQPPHIDYFEEIYVVTELMQSD
LHKIIVSPQPLSSDHVKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSN
CVLKICDFGLARVEELDESRHMTQEVVTQYYRAPEILMGSRHYSNAIDIW
SVGCIFAELLGRRILFQAQSPIQQLDLITDLLGTPSLEAMRTACEGAKAH
ILRGPHKQPSLPVLYTLSSQATHEAVHLLCRMLVFDPSKRISAKDALAHP
YLDEGRLRYHTCMCKCCFSTSTGRVYTSDFEPVTNPKFDDTFEKNLSSVR
QVKEIIHQFILEQQKGNRVPLCINPQSAAFKSFISSTVAQPSEMPPSPLV
WE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3846
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GSt for mammalian expression.; Calc Mol Weight : 84441 Daltons