cDNA Clone DAZ

• Plasmid: pEBG6P-DAZ
• Parent Plasmid: pEBG6P
• Expressed: GST-DAZ
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTCTGCTGCAAATCCTGAGACTCCAAACTCAACCATCTCCAG
AGAGGCCAGCACCCAGTCTTCATCAGCTGCAGCTAGCCAAGGCTGGGTGT
TACCAGAAGGCAAAATCGTGCCAAACACTGTTTTTGTTGGTGGAATTGAT
GCTAGGATGGATGAAACTGAGATTGGAAGCTGCTTTGGTAGATACGGTTC
AGTGAAAGAAGTGAAGATAATCACGAATCGAACTGGTGTGTCCAAAGGCT
ATGGATTTGTTTCGTTTGTTAATGACGTGGATGTCCAGAAGATAGTAGGA
TCACAGATACATTTCCATGGTAAAAAGCTGAAGCTGGGCCCTGCAATCAG
GAAACAAAAGTTATGTGCTCGTCATGTGCAGCCACGTCCTTTGGTAGTTA
ATCCTCCTCCTCCACCACAGTTTCAGAACGTCTGGCGGAATCCAAACACT
GAAACCTACCTGCAGCCCCAAATCACGCCGAATCCTGTAACTCAGCACGT
TCAGGCTTACTCTGCTTATCCACATTCACCAGGTCAGGTCATCACTGGAT
GTCAGTTGCTTGTATATAATTATCAGGAATATCCTACTTATCCCGATTCA
GCATTTCAGGTCACCACTGGATATCAGTTGCCTGTATATAATTATCAGCC
ATTTCCTGCTTATCCAAGATCACCATTTCAGGTCACTGCTGGATATCAGT
TGCCTGTATATAATTATCAGGCATTTCCTGCTTATCCAAATTCACCATTT
CAAGTCGCCACTGGATATCAGTTCCCTGTATACAATTATCAGCCATTTCC
TGCTTATCCAAGTTCACCATTTCAGGTCACTGCTGGATATCAGTTGCCTG
TATATAATTATCAGGCATTTCCTGCTTATCCAAATTCACCATTTCAAGTC
GCCACTGGATATCAGTTCCCTGTATACAATTATCAGGCATTTCCTGCTTA
TCCAAATTCACCAGTTCAGGTCACCACTGGATATCAGTTGCCTGTATACA
ATTATCAGGCATTTCCTGCTTATCCAAATTCAGCAGTTCAGGTCACCACT
GGATATCAGTTCCATGTATACAATTACCAGATGCCACCGCAGTGCCCTGT
TGGGGAGCAAAGGAGAAATCTGTGGACCGAAGCATACAAATGGTGGTATC
TTGTCTGTTTAATCCAGAGAAGAGACTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSAANPETPNSTI
SREASTQSSSAAASQGWVLPEGKIVPNTVFVGGIDARMDETEIGSCFGRY
GSVKEVKIITNRTGVSKGYGFVSFVNDVDVQKIVGSQIHFHGKKLKLGPA
IRKQKLCARHVQPRPLVVNPPPPPQFQNVWRNPNTETYLQPQITPNPVTQ
HVQAYSAYPHSPGQVITGCQLLVYNYQEYPTYPDSAFQVTTGYQLPVYNY
QPFPAYPRSPFQVTAGYQLPVYNYQAFPAYPNSPFQVATGYQFPVYNYQP
FPAYPSSPFQVTAGYQLPVYNYQAFPAYPNSPFQVATGYQFPVYNYQAFP
AYPNSPVQVTTGYQLPVYNYQAFPAYPNSAVQVTTGYQFHVYNYQMPPQC
PVGEQRRNLWTEAYKWWYLVCLIQRRD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU3735
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.; Calc Mol Weight : 71465 Daltons