cDNA Clone GCK

• Synonyms: MAP4K2
• Plasmid: pGEX6P-3-GCK corrected
• Parent Plasmid: pGEX6P3
• Expressed: GST-GCK
• Genbank: BC047865
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACGCGCTGCTGCGGGATGTGTCGCTGCAGGACCCGCGGGACCGCTT
CGAGCTGCTGCAGCGCGTGGGGGCCGGGACCTATGGCGACGTCTACAAGG
CCCGCGACACGGTCACGTCCGAACTGGCCGCCGTGAAGATAGTCAAGCTA
GACCCAGGGGACGACATCAGCTCCCTCCAGCAGGAAATCACCATCCTGCG
TGAGTGCCGCCACCCCAATGTGGTGGCCTACATTGGCAGCTACCTCAGGA
ATGACCGCTTGTGGATCTGCATGGAGTTCTGCGGAGGGGGCTCCCTGCAG
GAGATTTACCATGCCACTGGGCCCCTGGAGGAGCGGCAGATTGCCTACGT
CTGCCGAGAGGCACTGAAGGGGCTCCACCACCTGCATTCTCAGGGGAAGA
TCCACAGAGACATCAAGGGAGCCAACCTTCTCCTCACTCTCCAGGGAGAT
GTCAAACTGGCTGACTTTGGGGTGTCAGGCGAGCTGACAGCGTCTGTGGC
CAAGAGGAGGTCTTTCATTGGGACTCCCTACTGGATGGCTCCCGAGGTGG
CTGCTGTGGAGCGCAAAGGTGGCTACAATGAGCTATGTGACGTCTGGGCC
CTGGGCATCACTGCCATTGAGCTGGGCGAGCTGCAGCCCCCTCTGTTCCA
CCTGCACCCCATGAGGGCCCTGATGCTCATGTCGAAGAGCAGCTTCCAGC
CGCCCAAACTGAGAGATAAGACTCGCTGGACCCAGAATTTCCACCACTTT
CTCAAACTGGCCCTGACCAAGAATCCTAAGAAGAGGCCGACAGCAGAGAA
GCTCCTGCAGCACCCGTTCACGACTCAGCAGCTCCCTCGGGCCCTCCTCA
CACAGCTGCTGGACAAAGCCAGTGACCCTCATCTGGGGACCCCCTCCCCT
GAGGACTGTGAGCTGGAGACCTATGACATGTTTCCAGACACCATTCACTC
CCGGGGGCAGCACGGCCCAGCCGAGAGGACCCCCTCGGAGATCCAGTTTC
ACCAGGTGAAATTTGGCGCCCCACGCAGGAAGGAAACTGACCCACTGAAT
GAGCCGTGGGAGGAAGAGTGGACACTACTGGGAAAGGAAGAGTTGAGTGG
GAGCCTGCTGCAGTCGGTCCAGGAGGCCCTGGAGGAAAGGAGTCTGACTA
TTCGGTCAGCCTCAGAATTCCAGGAGCTGGACTCCCCAGACGATACCATG
GGAACCATCAAGCGGGCCCCGTTCCTAGGGCCACTCCCCACTGACCCTCC
AGCAGAGGAGCCTCTGTCCAGTCCCCCAGGCCCCAACAGCTCCCCACTGC
TGCCCACGGCCTGGGCCACCATGAAGCAGCGGGAGGATCCTGAGAGGTCA
TCCTGCCACGGGCTCCCCCCAACTCCCAAGGTGCATATGGGCGCCTGCTT
CTCCAAGGTCTTCAATGGCTGCCCCCTGCGGATCCACGCTGCTGTCACCT
GGATTCACCCTGTTACTCGGGACCAGTTCCTGGTGGTAGGGGCCGAGGAA
GGCATCTACACACTCAACCTGCATGAACTGCATGAGGATACGCTGGAGAA
GCTGATTTCACATCGCTGCTCCTGGCTCTACTGCGTGAACAACGTGCTGC
TGTCACTCTCAGGGAAATCCACGCACATCTGGGCCCATGACCTCCCAGGC
CTGTTTGAGCAGCGGAGGCTACAGCAACAGGTTCCCCTCTCCATCCCCAC
CAACCGCCTCACCCAGCGCATCATCCCCAGGCGCTTTGCTCTGTCCACCA
AGATTCCTGACACCAAAGGCTGCTTGCAGTGTCGTGTGGTGCGGAACCCC
TACACGGGTGCCACCTTCCTGCTGGCCGCCCTGCCCACCAGCCTGCTCCT
GCTGCAGTGGTATGAGCCGCTGCAGAAGTTTCTGCTGCTGAAGAACTTCT
CCAGCCCTCTGCCCAGCCCAGCTGGGATGCTGGAGCCGCTGGTGCTGGAT
GGGAAGGAGCTGCCGCAGGTGTGTGTTGGGGCCGAGGGGCCTGAGGGGCC
CGGCTGCCGCGTCCTGTTCCATGTCCTGCCCCTGGAGGCTGGCCTGACGC
CCGACATCCTCATCCCACCTGAGGGGATCCCAGGCTCGGCCCAGCAGGTG
ATCCAGGTGGACAGGGACACAATCCTAGTCAGCTTTGAACGCTGTGTGAG
GATTGTCAACATGCAGGGCGAGCCCACGGCCACACTGGCACCTGAGCTGA
CCTTTGATTTCCCCATCGAGACTGTGGTGTGCCTGCAGGACAGTGTGCTG
GCCTTCTGGAGCCATGGGATGCAAGGCCGAAGCCTGGATACCAATGAGGT
GACCCAGGAGATCACAGATGAAACAAGGATCTTCCGAGTGCTTGGGGCCC
ACAGAGACATCATCCTGGAGAGCATTCCCACTGACAACCCAGAGGCGCAC
AGCAACCTCTACATCCTCACGGGCCACCAGAGCACCTACTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDALLRDVSLQDPRD
RFELLQRVGAGTYGDVYKARDTVTSELAAVKIVKLDPGDDISSLQQEITI
LRECRHPNVVAYIGSYLRNDRLWICMEFCGGGSLQEIYHATGPLEERQIA
YVCREALKGLHHLHSQGKIHRDIKGANLLLTLQGDVKLADFGVSGELTAS
VAKRRSFIGTPYWMAPEVAAVERKGGYNELCDVWALGITAIELGELQPPL
FHLHPMRALMLMSKSSFQPPKLRDKTRWTQNFHHFLKLALTKNPKKRPTA
EKLLQHPFTTQQLPRALLTQLLDKASDPHLGTPSPEDCELETYDMFPDTI
HSRGQHGPAERTPSEIQFHQVKFGAPRRKETDPLNEPWEEEWTLLGKEEL
SGSLLQSVQEALEERSLTIRSASEFQELDSPDDTMGTIKRAPFLGPLPTD
PPAEEPLSSPPGPNSSPLLPTAWATMKQREDPERSSCHGLPPTPKVHMGA
CFSKVFNGCPLRIHAAVTWIHPVTRDQFLVVGAEEGIYTLNLHELHEDTL
EKLISHRCSWLYCVNNVLLSLSGKSTHIWAHDLPGLFEQRRLQQQVPLSI
PTNRLTQRIIPRRFALSTKIPDTKGCLQCRVVRNPYTGATFLLAALPTSL
LLLQWYEPLQKFLLLKNFSSPLPSPAGMLEPLVLDGKELPQVCVGAEGPE
GPGCRVLFHVLPLEAGLTPDILIPPEGIPGSAQQVIQVDRDTILVSFERC
VRIVNMQGEPTATLAPELTFDFPIETVVCLQDSVLAFWSHGMQGRSLDTN
EVTQEITDETRIFRVLGAHRDIILESIPTDNPEAHSNLYILTGHQSTY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3643
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.